Nikon objectives used in optics and photonics applications are available at Edmund Optics.

Priming means putting a dose of medicine on the inside of the spacer, to reduce static. This is so the medicine won't stick to the spacer. When you get a new spacer read the instructions to see if it needs priming before you first use it and how you should prime your spacer.

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

Microscopeparts

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

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These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

Objectivelens microscopefunction

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

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If you’re using a dry powder Inhaler (DPI) or a breath-actuated inhaler (BAI), you won’t need to use a spacer. Speak to your GP, nurse, or pharmacist if you’re not sure which type of inhaler you’re using.

Both techniques work equally well. Multiple breathing may be prescribed to younger children as it’s easier to teach. Your GP can tell you if there’s a technique that will be better for you.

For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

Your GP, nurse or pharmacist should show you how to use your inhaler and spacer properly. You can also check your technique at your annual asthma review.

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Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

When you’re not using your spacer, storing it properly will keep it in good condition, helping you or your child to get the best from your medicine.

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Using a spacer with your metered dose inhaler (MDI) helps the right amount of medicine get to your lungs. Using a spacer will mean:

You should replace your spacer at least every year, especially if you use it daily, but some may need to be replaced sooner – ask your GP, nurse or pharmacist if you’re unsure.

Find out the benefits of using a spacer with your inhaler, how to look after your spacer, and how adults, children, and babies can use them properly.

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

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These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

When you're not using your spacer, storing it properly will keep it in good condition. Keep your spacer away from dust and liquids. Keep your spacer in a breathable bag such as a cotton or cloth bag, so it doesn't get scratched and so small objects don't get stuck inside it. Don't put your spacer in a plastic bag as this will cause it to build up static. Children could decorate a plastic-free medicine bag or a fabric pencil case so they're more likely to use it.

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

Spacers are empty tubes that are usually made from plastic. You slot your inhaler into one end of the spacer and breathe the medicine in through the other end.

To clean your spacer, first take your spacer apart and gently clean it with warm water and a detergent, such as washing up liquid. Although most spacers are cleaned in the same way, check the instructions for your spacer. Only a small number of brands of spacer are dishwasher safe. Use warm water instead of boiling water, as boiling water may damage the spacer. Be careful not to scrub the inside of your spacer, as this can affect the way it works.

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

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Some spacers need rinsing. With others, it's best to leave a soapy film, which prevents static. Static can make the medicine stick to the sides so it's hard to breathe in. If you're not sure, check the information leaflet that comes with your spacer.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

Microscopeparts and functions

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

Most spacers need replacing every year, depending on how often you use it. Some spacers may need to be replaced sooner. Ask your GP or pharmacist if you're not sure. Spacers are not widely recycled, so you may need to dispose of it in the normal household waste, but check the information leaflet that comes with your spacer to find out how to dispose of it safely.

Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

If you’re using a spacer with rubber valves, replace it if the valves become stiff or are starting to crumble (become brittle).

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These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

If you have any questions about looking after your spacer, speak to your doctor, nurse, or pharmacist. You can also call our helpline.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

Check the information leaflet that comes with your spacer to find out how to dispose of it. Spacers are not widely recycled so you may need to dispose your spacer in normal household waste.

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Speak to your GP, nurse or pharmacist about finding the right spacer for you. You can buy a spacer from your pharmacy without a prescription too.

A spacer is a plastic tube that fits onto the mouthpiece of your inhaler. It helps you breathe the medicines into your lungs more easily. Spacers are used with pressurised metered dose inhalers or pMDIs. Keeping your spacer clean and in good condition helps make sure it works properly and you get the best from your medicines. When you first get a new spacer, clean it before you use it. After that you should usually clean your spacer once a month.

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

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For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

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To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

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For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

What is objectivelensinmicroscope

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

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Hi I'm Leanne, a respiratory nurse specialist at Asthma and Lung UK. I'm going to tell you how to clean and store your spacer.

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

•    Watch our video to help you do the ‘tidal or multiple breathing’ technique. •    Watch our video to help you do the ‘single breath and hold’ technique.

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