A scanning objective lens provides the lowest magnification power of all objective lenses. 4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of 40x. The name “scanning” objective lens comes from the fact that they provide observers with about enough magnification for a good overview of the slide, essentially a “scan” of the slide. Some objectives with even lower power are discussed in Specialty Objectives below.

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The low power objective lens has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples. The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.

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Polarization is important in wireless communications systems. The physical orientation of a wireless antenna corresponds to the polarization of the radio waves received or transmitted by that antenna. Thus, a vertical antenna receives and emits vertically polarized waves, and a horizontal antenna receives or emits horizontally polarized waves. The best short-range communications is obtained when the transmitting and receiving (source and destination) antennas have the same polarization. The least efficient short-range communications usually takes place when the two antennas are at right angles (for example, one horizontal and one vertical). Over long distances, the atmosphere can cause the polarization of a radio wave to fluctuate, so the distinction between horizontal and vertical becomes less significant.

Most compound microscopes come with interchangeable lenses known as objective lenses. Objective lenses come in various magnification powers, with the most common being 4x, 10x, 40x, and 100x, also known as scanning, low power, high power, and (typically) oil immersion objectives, respectively. Let’s take a closer look at each of the different magnifications of objective lenses and when you would use them.

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Some wireless antennas transmit and receive EM waves whose polarization rotates 360 degrees with each complete wave cycle. This type of polarization, called elliptical or circular polarization, can be either clockwise or counterclockwise. The best communications results are obtained when the transmitting and receiving antennas have the same sense of polarization (both clockwise or both counterclockwise). The worst communications usually takes place when the two antennas radiate and receive in the opposite sense (one clockwise and the other counterclockwise).

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If you are interested in buying various types of objective lenses for your microscope in the classroom, laboratory, research facility, or any other purpose, ACCU-SCOPE can provide the products you are looking for. Contact us today to learn more about our objective lenses and other microscope accessories.

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There are several other objective lens magnifications available with utility for particular applications. The 2x objective, widely used in pathology, has only ½ the magnification of a 4x scanning lens, thus providing a better overview of the sample on the slide. The 50x oil immersion objective, often used in place of the 40x objective, is used as a gold standard for observing blood smears. The 60x objective, often available in either dry or oil immersion, provides 50% greater magnification than a 40x lens. The 60x dry is sometimes chosen over a 100x oil immersion lens for higher magnification without the need to use immersion oil.  Finally the 100x dry objective doesn’t need immersion oil to deliver high magnification (still 1000x when combined with 10x eyepieces). However, the numerical aperture (an indication of resolving power of an objective) of a 100x dry objective is much lower than that of a 100x oil immersion objective and, as a result, the ability of the lens to resolve fine details in the specimen is much lower, too.

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The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample. The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.

Polarization, also called wave polarization, is an expression of the orientation of the lines of electric flux in an electromagnetic field ( EM field ). Polarization can be constant -- that is, existing in a particular orientation at all times, or it can rotate with each wave cycle.

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The oil immersion objective lens provides the most powerful magnification, with a whopping magnification total of 1000x when combined with a 10x eyepiece. But the refractive index of air and your glass slide are slightly different, so a special immersion oil must be used to help bridge the gap. Without adding a drop of immersion oil, the oil immersion objective lens will not function correctly, the specimen will appear blurry, and you will not achieve an ideal magnification or resolution. Oil immersion lenses are also available from some manufacturers in lower magnifications, and provide higher resolution than their "high dry" counterparts.

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Polarization affects the propagation of EM fields at infrared ( IR ), visible, ultraviolet ( UV ), and even X-ray wavelength s. In ordinary visible light, there are numerous wave components at random polarization angles. When such light is passed through a special filter, the filter blocks all light except that having a certain polarization. When two polarizing filters are placed so a ray of light passes through them both, the amount of light transmitted depends on the angle of the polarizing filters with respect to each other. The most light is transmitted when the two filters are oriented so they polarize light in the same direction. The least light is transmitted when the filters are oriented at right angles to each other.

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It is important to always use the correct immersion media (e.g. air, water, oil, etc.) that is specified by your objective lens.