Silicon lens ; Doping Element Concentration(1/cm3). ≦1×10E17 ; Coefficient of thermal expension. 2.5(T=300k) ; Linear Expension Coefficient. (2-9)×10-6 k-1.

Class 4laser

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Open your image in FIJI, find out the pixel size of the image from the table. Go to Image>Properties and enter the pixel width and pixel height.

Gently rub your lenses with a lens cloth, wiping away the solution as you clean. Using a lens cleaner is a great option to make sure your lenses are streak-free ...

@mani2263 If you have some sort of point sensor you may have different pixel width/height which could be adjusted with Image>Properties (shortcut “P”), see Your screenshot shows 2048 x 1536 pixel which are most probably taken by some CMOS/CCD sensor and it is very unlikely that horizontal and vertical resolution are not the same or at least very similar and a resolution 2412 dpi vs 3175 dpi is implausible. At 3175 dpi 1 pixel equals 8 µm which leads to a 1-pixel-structure of 8 x 8 µm or 64 µm2 which is far away from nanoscale.

Class 2laser eye damage

Owning a super-bright one (e.g. 30mW or higher) is something I would consider awfully risky. Treat a super-bright green laser pointer just like you would treat a circular saw: You store it well out of reach of children (and out of reach of irresponsible adults!), you leave it unplugged / remove the batteries when it's not in use, you don't use it if you're drunk, you don't have fun with it, etc. etc. etc.

You also should not assume the power rating is correct. It says 5mW probably because that's the most desired power level that consumers want: As bright as possible without being obviously illegal. But really it could be 2mW, or 10mW, or who knows.

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Is a Class 2laserdangerous

so this looks that it should not be Class IIIB, also because the power is less than 5mW while according to Wikipedia the "B" class should be for 5-200 mW.

optic-10759-Oslon_Pure_1010_Cyan-cross-section-image-vertical.jpg. Download. IES · Details. Osram OSLON Pure 1010 CSP GD VJLPE1.14 Deep Blue. Eff: 81.0%. FWHM:.

Every laser pointer is dangerous, is not a toy, and should not be pointed anywhere close to other people ...but it takes extreme and unusual bad luck to cause permanent eye damage if its labeling is correct and it is really under 5mW (Note: I am talking specifically about visible-light, continuous-wave green lasers here). Here is a literature review, and you'll see that some injuries were indeed caused by laser pointers with less than 5mW of power, but most were injured by much more powerful lasers, like 30mW and higher.

200181 — Edmund Salvage became Edmund Scientific in the late 1940s and continued to specialize in optics. Edmund made regular trips to Japan ...

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Finally, stepping back, the review cited above found 111 patients with eye injuries from green laser pointers. Given how many green laser pointers are around, I wouldn't consider owning a legally-compliant green laser pointer to be a notably risky activity, compared to other day-to-day risks.

Class 3laser eye damage

Olympus Fluoview-1000 Manual Online: size of pixels, Table. 5 Pixel Size (Μm) Versus Box Size And Magnification At Zoom = 1. The Magnification And Pixel Size Of An Image Is A Function Of Lens Magnification, Box Size And Zoom Factor. However, These...

The classification is dependent on wavelength, and the potential for damage. I would heed the classification, it is right on the edge of safe.

Class 1laser

Realistically it is unlikely to cause damage unless the beam is incident directly into the eye, but damaging the eyes with lasers is pretty much irreversible and will result in vision loss (usually just partial loss). Lasers in the visible range damage the retina, which is what makes them so dangerous.

The width would be 1392 pixel / 96 dpi (dots per inch = pixel per inch) = 14.5 inch. Be aware that “96 dpi” is a very common standard setting and may be completely wrong, e.g. in pictures from digital consumer cameras.

Class 3Blaser

I am new to Fiji, and I’m working with .oib images taken with an Olympus FV1000 confocal microscope. Most images were taken using 10x magnification, and some were taken using 4x. I need to add scale bars to the images, but I’m not sure how to identify what the scale is on the images. I’ve opened Image → Show Info, but I’m not sure how to use this information to set the scale and insert a scale bar. I know that I’m supposed to go to Analyze → Set Scale use the straight line selection tool to make a line selection that corresponds to a known distance, but how do I do this? I’m a bit confused.

You would use the procedure you describe when you need to set the scale from the known size of an object in the image and not from prior knowledge.

look at the table of pixel sizes by magnification and field size of your confocal microscope, like for example this one:

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@psobolewskiPhD Thanks for your time. I have one more query; what if x and y dimension of the image are not same? As with the image I am sharing info of. I calibrated on X-axis (pixels/um), but when i measured the feature, it was in nano scale which is beyond the resolution of optical microscope. Means there is something wrong, we have to adjust y-axis too. Any idea on that? ImageJ only takes combined x,y-axis calibration in pixels/um. It does’nt treat both axis seperately!

Laserpointer Distancechart

Classs IIIb lasers may be harmful to your eyes because their range is from 5mW-500mW. In addition, this laser can represent a fire hazard and may burn your skin lightly.

F-number is defined as the lens focal length (f) divided by the lens aperture diameter (d). It defines the size of the cone of light that is ...

Thanks for all the help. I have just one last question: How can I calculate the width and height of an immagine, when the pixel width and height are not shown in the table above? The datapoints of my immagine are these:

First and foremost, you should know that the legal restrictions on laser pointers are not enforced. Therefore you should not assume the class labelling is correct. One study found that 90% of green lasers in the US had an illegally high power level.

We just bought a green laser pointer on Ebay and had a discussion about the safety. The laser is low end chinese one (5 USD, free shipping :-) ) and the seller says this:

Class 4laserpointer

the most confusion comes from the "Class IIIB" which, according to Wikipedia should be not safe for common use because it can damage eye even with accidential look or reflection.

I do not intend to shine anybody in the eyes but my sister was afraid that there could be potential risk to damage someones eyes with accidential quick exposure (reflection from window in a class or directly from the chalkboard to the class). The green pointer seems to have very intensive light compared to the red ones but it could be a subjective impression caused by the different color.

Only you–or the microscopist–can know this. The image capture software should put everything it can account for in the metadata, but it’s always possible something else is affecting the scale, like an additional lens in the path to the camera or something. It’s always a good idea to sanity check with something that you know how big it should be. If you do have an extra factor you need to multiple (or divide) the number you enter into the Set scale... dialog so the values represent reality: how many pixels represent a known distance in microns (or whatever unit you’re using)

I have a problem, Could you help? I took photos at x10, and then the photos came with scale bar 500um How can I remove this and calculate the correct measurement?

I think there is rather an incorrect information on the selling page and that the pointer should be safe but I am rather asking to be sure.

35mm equivalent Focal Length Multiplier Calculator This calculator allows you to calculate the 35mm equivalent Focal Length for digital camera at specific ...

So in this case, my image size is 1024 x 1024, I believe, and the zoom is 10x. So then, based on the table, I would enter in the Set Scale window “Distance in pixels: 1” and “Known distance: 1.242” with unit being μm. This should give me the correct scale so I can insert the scale bar?

Thanks a lot peter, just need 1 more clarification; this info from metadata caters for magnification or we have to apply some scaling factor?. if it is so, then how to apply scaling factor? Thank you

That image info screenshot has everything that you need: You can either use: Resolution: 0.8052 pixels per um Which means in the Set Scale (see below) you enter 0.8052 to Distance in pixels and 1 in Known distance and um in Unit of length Or you can can use the information from: Width: 1271.808 um (1024) you enter 1024 to Distance in pixels and 1271.808 in Known distance and um in Unit of length

Vapour retarders also are commonly referred to simply as vapour barriers. The barrier terminology is less accurate because, in most cases, the products don't ...

Personally (and I work with lasers often) I would use the one that you purchased for Astronomical pointing (i.e., star gazing) only. If I wanted to do something else I would get a lower power laser, it isn't worth the risk of eye damage...