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Record the magnification of the ocular lens in the eyepiece. The magnification of the ocular lens is typically engraved on the side of the eyepiece.

Record the magnification of the objective lens. The magnification is frequently engraved along with the numerical aperture (NA) on the side of the objective lens. Many compound light microscopes allow different objective lenses to be rotated into use on the nosepiece. Each objective lens has a different magnification.

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Chandler, David. "How To Determine Magnification Of A Microscope" sciencing.com, https://www.sciencing.com/determine-magnification-microscope-6293409/. 9 March 2018.

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refractive index, measure of the bending of a ray of light when passing from one medium into another. If i is the angle of incidence of a ray in vacuum (angle between the incoming ray and the perpendicular to the surface of a medium, called the normal) and r is the angle of refraction (angle between the ray in the medium and the normal), the refractive index n is defined as the ratio of the sine of the angle of incidence to the sine of the angle of refraction; i.e., n = sin i / sin r. Refractive index is also equal to the velocity of light c of a given wavelength in empty space divided by its velocity v in a substance, or n = c/v.

Some typical refractive indices for yellow light (wavelength equal to 589 nanometres [10−9 metre]) are the following: air, 1.0003; water, 1.333; crown glass, 1.517; dense flint glass, 1.655; and diamond, 2.417. The variation of refractive index with wavelength is the source of chromatic aberration in lenses. The refractive index of X-rays is slightly less than 1.0, which means that an X-ray entering a piece of glass from air will be bent away from the normal, unlike a ray of light, which will be bent toward the normal. The equation n = c/v in this case indicates, correctly, that the velocity of X-rays in glass and in other materials is greater than its velocity in empty space.

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The magnification of a microscope describes the increase in apparent size of an object compared with its actual size. An object magnified 10 times (10X) appears 10 times larger than it really is. Total magnification is the product of the ocular lens magnification and the objective lens magnification. Magnification does not describe the quality of the image. Magnifying an object without good resolution is called empty magnification, as the image appears larger but no greater detail can be seen. Resolution typically limits the usefulness of light microscopes rather than magnification.

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Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of 400X (10 x 40 = 400). Changing the ocular lens or objective lens to lenses with a different magnification will change the total magnification of the microscope. Usually, it is the objective lens that is changed to increase or decrease magnification.

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Chandler, David. How To Determine Magnification Of A Microscope last modified March 24, 2022. https://www.sciencing.com/determine-magnification-microscope-6293409/

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Chandler, David. (2018, March 9). How To Determine Magnification Of A Microscope. sciencing.com. Retrieved from https://www.sciencing.com/determine-magnification-microscope-6293409/