High Power Laser Polarizing Cube Beamsplitters (HPB) - polarizing beamsplitters

 2024-07-16 03:27:13

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F10 fresnelvsf10

All these techniques put many different demands on the excitation sources being used, in terms of wavelengths, power levels, power modulation, beam quality and spectral characteristics. A common factor across most techniques is the typically need for many excitation wavelengths in order to address a continuously increasing number of fluorophores and to achieve multi-color imaging.

Publication A. Rohbach et al. 100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales. Nature Communications 13, Article number: 1758 (2022)

High power, single frequency, CW diode pumped lasers Wavelength: 320 nm – 1064 nm Power: 10 mW – 3000 mW Applications: Holography, Raman, microscopy, flow cytometry, research

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Most microscope set-ups with multi-color excitation capability typically use multiple individual lasers combined through optical elements and coupled into one or more output beams or optical fibers. Such a laser combiner offers the greatest flexibility in all respects, many wavelengths, many power levels, as well as fast and slow modulation. However, for systems and set-ups where flexibility is not the highest priority, a multi-line laser can offer a more permanently aligned, easier to use and maintenance free alternative.

Aputuref10 fresnelcase

The development of compact, reliable solid-state lasers was an initial enabling technology for commercialization and expansion of high-resolution fluorescence microscopy techniques to new markets and applications, accompanied by parallel improvements in data storage and advanced camera systems, to name a few. While some microscope applications are able to utilize the advancements in LED and super-continuum white-light sources; the high-resolution, high-speed techniques like confocal microscopy still rely on the high-brightness and wavelength precision of lasers.

In the HÜBNER Photonics division, the principles of high-quality workmanship, superior reliability and lifetime are applied to the building of advanced tools and solutions.

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Publication A. Tengholm et al. Fluorescent protein vectors for pancreatic islet cell identification in live cell imaging 2016

Publication M. Stefaniuk et al. Light sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene Nature 2015

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Dual-Optic DesignThe F10 Fresnel matches the unique design of the Fresnel 2X, utilizing a two-stage optical system to maximize light output. Utilizing a rear aspherical focusing element, the F10 Fresnel optimizes the output of an LS 600d Pro to fill the front Fresnel Element, resulting in up to 340% additional output in Flood mode.

Publication S. Barg et al. Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site Nature 2014

Through the well-known Swedish laser manufacturer Cobolt AB, a proven supplier of high performance lasers of more than 15 years, HÜBNER Photonics division offers one of the industry’s broadest ranges of compact single-frequency CW lasers, diode lasers, multi-line lasers and Q-switched lasers across the full UV-Visible-NIR spectrum.

Over the last decade, fluorescence based life science research has been revolutionized by new imaging methods and the transitioning from bulky gas-laser sources into solid-state lasers with a smaller footprint, longer lifetime, and lower maintenance requirements.

Modern fluorescence microscopy consists of an enormous variety of different techniques ranging from standard laser scanning confocal microscopy, TIRF and spinning-disc microscopy to light sheet microscopy and various approaches for super-resolution imaging using spatial manipulation of the fluorescence signal.

One of the most important tools for microbiology research is high-resolution live-cell imaging through fluorescence microscopy, in which certain molecules, so-called fluorochromes or fluorophores, return low-energy light after excitation with light of a defined wavelength, i.e. light with a higher wavelength than the excitation light. Scientists are taking advantage of this physical effect to investigate ever smaller structures visible in biological processes. Striving to increase the resolution has led to the need not only to use special microscopes but also suitable light sources such as lasers of different wavelengths. In particular, the exact selection of suitable lasers enables the temporal and local resolution to be increased. Lasers are an integral part of modern fluorescence microscopy!

Impeccable BuildDespite its impressive 250mm (9.84in.) Fresnel diameter, the F10 Fresnel maintains a compact heat-resistant construction. The shell of the F10 can withstand the heat of the LS 600d Pro, and due to its collapsible, and aspherical focusing lens, it can stow away to a depth of 6.8 inches (172.5mm).Drop-in Accessory SlotAnother of many "firsts", the F10 Fresnel is also the first Bowens Mount Fresnel to feature an industry-standard drop-in accessory slot. This heavy-duty accessory slot allows users to mount the F10 Barn Doors on the Fresnel for proper light shaping, and can even fit a 13-inch (330mm) scrim.Durable Carrying BagMade to follow you onto any film set, the F10 Fresnel comes packed inside a heavy-duty carrying bag for easy travel.

HÜBNER Photonics GmbH Wilhelmine-Reichard Strasse 6 34123 Kassel, Germany Phone: +49 561 994 060 – 0 Technical Support: +49 561 994 060 – 12 Fax: +49 561 994 060 – 13

The compact and flexible laser combiner Wavelength: 375 nm – 1064 nm Power: 50 mW – 1000 mW Applications: Microscopy, Raman, holography

Aputure 1200d

Welcome to our Technology Vision for. 2024. This year's Vision is grounded in two realities. First, technology is driving a wave.

An example of an image taken in a single-molecule localization microscopy (SMLM) setup including Cobolt Skyra™ from Department of Biotechnology & Biophysics at Julius-Maximilian-University of Würzburg.

Intensely BrightThe F10 Fresnel functions at its best when paired with the Aputure LS 600d Pro, a 600W Daylight COB LED. Together, this combination eclipses Studio 2K Tungsten lights, and approaches the intensity of 5K Tungsten fixtures at flood. With a combined flood output of 11,440 lux and spot output of 29,300 lux at 3 meters, The 600d Pro and F10 Fresnel even compete on par with higher-powered HMIs.

Ultrafast femtosecond fiber lasers Pulse duration: <50 fs Power: Up to 2 W Applications: Multiphoton Microscopy, Two-photon Polymerization, Optogenetics

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AputureF10 Fresnelcompatibility

The First EverThe Aputure F10 Fresnel is the first industry-standard 10-inch Fresnel made for modular Bowens Mount fixtures. With its wide 250mm (9.84in.) glass Fresnel, the F10 both immensely intensifies the output of a point-source LED, but also results in a quality of light lighting technicians will find similar to a Tungsten 5K or Studio 2K.

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Sep 4, 2012 — If you get a regular magnifier or a microscope then you want 30x, 40x, or 60x depending on the detail your after. However a jeweler's loupe ...

Single frequency, CW diode pumped lasers Wavelength: 457 nm – 1064 nm Power: 25 mW – 400 mW Applications: Raman, microscopy, LDV, DLS

Plug & play modulated CW lasers Wavelength: 375 nm – 1064 nm Power: 40 mW – 400 mW Applications: Microscopy, flow cytometry, optogenetics

Cobolt AB Visit: Vretenvägen 13 Delivery: Hemvärnsgatan 20 171 54 Solna, Sweden Phone: +46 8 545 912 30 Fax: +46 8 545 912 31

Minimized Light LeaksBuilding on our experience from the Fresnel 2X, the F10 Fresnel uses an offset ventilation design in its heat-resistant body that allows for airflow while minimizing light leaks.

F10 fresnelreview

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AputureFresnel2X

Recent laser-based imaging methods try to combine different techniques such as cLSM and Raman spectroscopy. The Cobolt lasers of the 04/05 and 08-01 Series are well suited for Raman spectroscopy due to the very narrow linewidth and excellent spectral purity. Our C-FLEX laser combiner is the perfect solution to combine both Cobolt 06-01 Series of plug & play diode lasers and the narrow linewidth lasers from the 04/05 and 08-01 Series, thus offering maximum flexibility for many Life science applications.

Introduction. Galvo Scanner (GS) is widely used for lateral laser scanning in various imaging modalities such as photoacoustic microscopy [1-3], confocal ...

The principal purpose of a polariser is to eliminate surface reflections, glare and hot spots from any light source entering the lens.

One way to increase the readout speed of a camera sensor is to use multiple taps on the sensor. This means that instead of all pixels being read out ...

AputureFresnel2X compatibility

F10 Fresnel1200d

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All Cobolt lasers can be integrated into very flexible and user-friendly laser combiner solutions on the C-FLEX platforms with up to 8 lasers in one combiner. The unique C-WAVE laser offers wide tunability across the almost the complete visible and near IR spectrum, enabling access to more exotic fluorophores. In addition, we can offer the Cobolt Skyra, which is an extremely compact, permanently aligned, multi-line laser that simplifies the integration of multi-color excitation in compact microscopy equipment by eliminating the need for in-field alignment and service, which reduces manufacturing cost and allows for more compact designs. The Cobolt Skyra can also be configured to make a perfect compact solid-state alternative to Ar-ion lasers.

At HÜBNER Photonics we have a very large selection of single and multi-line lasers by Cobolt, perfect for fluorescence microscopy applications. The fast modulation capabilities of our Cobolt 06-01 laser diodes in particular make them very suited to all confocal systems such as cLSM, Spinning Disc and TIRFM. In addition, the excellent on/off modulation, including true off, make them even more attractive for applications such as Photoactivation, Photoconversion, Optogenetics, Laser manipulation, FRET, FRAP, to name just a few. In addition, they are also suitable for super-resolution microscope techniques such as STORM, PALM and STED, since these lasers are available over a wide wavelength range even with high powers.

Publication I. Rocha-Mendoza et al. Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters 2015

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A revolutionary multi-line laser platform Wavelength: 405 nm – 685 nm Power: 50 mW, 100 mW Applications: Microscopy, flow cytometry

Ergonomic FocusingTo ergonomically and precisely adjust its variable 15°-45° beam angle, the F10 Fresnel uses Aputure’s helicoid focus design introduced in the Fresnel 2X. This seamless design requires no extra knobs or handles. Simply twist the lens ring, and adjust the spot/flood controls.

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Description. LZR-532Kit– 532nm Green Laser with battery, charger, adjustable rings and universal mounting bracket. This laser has been tested under extreme cold ...