Objectivelens microscope function

The stereo- or dissecting microscope is an optical microscope variant designed for observation with low magnification (2 - 100x) using incident light illumination (light reflected off the surface of the sample is observed by the user), although it can also be combined with transmitted light in some instruments. It uses two separate optical paths with two objectives and two eyepieces to provide slightly different viewing angles to the left and right eyes. In this way it allows a three-dimensional visualization of the sample.

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Ocularlens magnification

Ocular objectivevs magnification

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The stereomicroscope should not be confused with a binocular compound microscope, which has double eyepieces. The image in such a binocular compound microscope is no different from that obtained with a single monocular eyepiece.

Objectivelens magnification

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Ocular objectiveexample

A compound microscope is a microscope that uses multiple lenses to enlarge the image of a sample. Typically, a compound microscope is used for viewing samples at high magnification (40 - 1000x), which is achieved by the combined effect of two sets of lenses: the ocular lens (in the eyepiece) and the objective lenses (close to the sample).

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Ocular objectivemicroscope

Compound microscopes usually include exchangeable objective lenses with different magnifications (e.g 4x, 10x, 40x and 60x), mounted on a turret, to adjust the magnification. These microscopes also include a condenser lens and iris diaphragm, which are important for regulating how light hits the sample.

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Light is passed through the sample (called transmitted light illumination). Larger objects need to be sliced to allow this to happen efficiently.

Great working distance and depth of field are important qualities for this type of microscope, allowing large specimens such as small animals, plants and organs to be viewed with most parts in focus at the same time. In addition to the ocular and objective lens, stereomicroscopes typically contain:

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