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These lower magnification objectives provide a wide field of view which is useful for scanning slides and locating specimens. Resolution and fine detail are low, but the overview of the sample is clear.
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Due to Fresnel reflection, as light passes from air through an uncoated glass substrate approximately 4% of the light will be reflected at each interface. This results in a total transmission of only 92% of the incident light, which can be extremely detrimental in many applications (Figure 1). Excess reflected light reduces throughput and can lead to laser-induced damage in laser applications. Anti-reflection (AR) coatings are applied to optical surfaces to increase the throughput of a system and reduce hazards caused by reflections that travel backwards through the system and create ghost images. Back reflections also destabilize laser systems by allowing unwanted light to enter the laser cavity. AR coatings are especially important for systems containing multiple transmitting optical elements. Many low-light systems incorporate AR coated optics to allow for efficient use of light.
Anti-reflection V-coats are a type of AR coating designed to increase transmission over a very narrow waveband centered at a specified design wavelength (DWL). This coating type is called “V-coat” because the curve of the transmission versus wavelength forms a “V,” with a minimum at the DWL. V-coats are ideal for obtaining maximum transmission when using single-frequency, small linewidth lasers, or narrow full width-half max (FWHM) light sources.1 V-coats typically have a reflectivity of less than 0.25% at the DWL. However, the reflection curve for the coating locally has a nearly parabolic shape and the reflectivity is significantly higher at wavelengths besides the DWL (Figure 3).
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Lower magnification gives a wide field of view to find and identify organisms. Medium magnifications like 400x are ideal for studying cell structure. 1000x is used for finer details like organelles.
The objective lenses on most compound microscopes typically come in 4x, 10x, 40x, and 100x magnifications. Some microscopes may also have a 60x or 150x objective.
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Because reflectivity increases rapidly as the wavelength of the source moves further away from the DWL, optical components with V-coats are meant for use at exactly or very close to the intended DWL of the coating. An interesting characteristic of V-coats is that the shape of their transmission curves is semi-periodic such that the reflectivity reaches a local minimum at harmonics of the DWL (e.g. $ \tfrac{\lambda_0}{2} $ or $ \tfrac{\lambda_0}{4} $) that are not as optimized for reflectivity as at the DWL. V-coats are usually comprised of only two coating layers. Simple V-coats can consist of a single layer with a thickness of a $ \tfrac{\lambda}{4} $, but more layers may be required to adjust the bandwidth or if a coating material with an appropriate index of refraction is not available. Multilayer coatings may also compensate for different angles of incidence, but are more complicated and tend to have larger bandwidths. If the thickness of the V-coat layers is incorrect, the reflectivity of the coating increases and the DWL changes. V-coats from Edmund Optics typically achieve minimum reflectivities significantly less than 0.25%, but all standard V-coats have specified reflectivities of <0.25% at the DWL. This allows for small shifts in the DWL from coating tolerances.
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To achieve the full NA of 1.25, immersion oil is placed between the slide and the 100x objective. This prevents refraction and loss of resolution from the air-glass interface.
The high NA paired with oil immersion allows the 100x objective to see fine subcellular details like organelles and chromosomes. It is used to study cellular structure and mitosis.
NIR I and NIR II: Our near-infrared I and near-infrared II broadband AR coatings offer exceptional performance in near-infrared wavelengths of common fiber optics, laser diode modules, and LED lights.
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Table 1 shows the reflectivity and guaranteed laser-induced damage threshold (LIDT) for Edmund Optics’ standard laser V-coats.
Edmund Optics offers all TECHSPEC® transmissive optics with a variety of anti-reflection (AR) coating options that vastly improve the efficiency of the optic by increasing transmission, enhancing contrast, and eliminating ghost images. Most AR coatings are also very durable, with resistance to both physical and environmental damage. For these reasons, the vast majority of transmissive optics include some form of anti-reflection coating. When specifying an AR coating to suit your specific application, you must first be fully aware of the full spectral range of your system. While an AR coating can significantly improve the performance of an optical system, using the coating at wavelengths outside the design wavelength range could potentially decrease the performance of the system.
The 10x objective lens typically has a numerical aperture of 0.25 and a focal length of 4mm. The field of view at 10x is around 2mm across.
Start with the lowest power 4x or 10x objective to locate the specimen. Then increase magnification to observe specific structures. Use the 100x oil immersion lens for the finest details.
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The 4x scanning objective has a numerical aperture of 0.10 and a focal length of 16mm. It has a field of view of 5mm across.
Plan apochromat objectives are high-quality optimized lenses that reduce optical aberrations and provide flat, sharp images. Fluorite lens elements allow a wider spectrum of light throughput. These advanced objectives paired with oil immersion provide the clearest high-magnification views.
Broadband anti-reflection (BBAR) coatings are designed to improve transmission over a much wider waveband. They are commonly used with broad-spectrum light sources and lasers with multiple-harmonic generation. BBAR coatings typically do not achieve reflectivity values quite as low as V-coats but are more versatile because of their wider transmission band. In addition to being applied to transmissive optical components including lenses and windows, AR coatings are also used on laser crystals and nonlinear crystals to minimize reflections, as Fresnel reflections occur where air and the crystal meet.1
UV-AR and UV-VIS: Ultraviolet coatings are applied to our UV fused silica lenses and UV fused silica windows to increase their coating performance in the ultraviolet region.
AR coatings are designed so that the relative phase shift between the beam reflected at the upper and lower boundaries of a thin film is 180°. Destructive interference between the two reflected beams occurs, which cancels out both beams before they exit the surface (Figure 2). The optical thickness of the optical coating must be an odd integer multiple of $\tfrac{\lambda}{4}$, where $ \small{\lambda} $ is the design wavelength or wavelength being optimized for peak performance in order to achieve the desired path difference of $\tfrac{\lambda}{2}$ between the reflected beams. When achieved, this will lead to the cancellation of the beams. The index of refraction of a thin film $ \small{\left( n_f \right)} $ needed for complete cancelation of the reflected beams can be found by using the refractive indices of the incident medium $ \small{\left( n_0 \right)} $ and the substrate $ \small{\left( n_s \right)} $.
Edmund Optics offers all TECHSPEC® lenses with an optional single-layer, dielectric anti-reflection (AR) coating to reduce surface reflections. In addition, custom single-layer, multi-layer, V, and 2V coatings are available for both our off-the-shelf and large volume custom orders. View Custom Optical Lens Coatings for information.
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The field of view at 40x is 0.5mm across – much smaller than the lower power lenses but still enough to see most cell details. 40x is commonly used for studying cell structures.
$\tfrac{\lambda}{4}$ MgF2: The simplest AR coating used is $ \tfrac{\lambda}{4} $ MgF2 centered at 550nm (with an index of refraction of 1.38 at 550nm). MgF2 coating is ideal for broadband use though it gives varied results depending upon the glass type involved.
Telecom-NIR: Our telecom/near-infrared is a specialized broadband AR coating for popular telecommunications wavelengths from 1200 – 1600nm.
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VIS 0° and VIS 45°: VIS 0° (for 0° angle of incidence) and VIS 45° (for 45° angle of incidence) provide optimized transmission for 425 – 675nm, reducing average reflection to 0.4% and 0.75% respectively. VIS 0° AR coating is preferred over MgF2 for visible applications.
Some microscopes may also have specialized oil immersion objectives like 60x or 150x. Like the 100x lens, these objectives require immersion oil to reach their maximum NA and magnifying power. Immersion oils are specially formulated to have specific refractive indexes matched to the lens.
The CaF2 crystal windows provide superior transmission in broad wavelength range from vacuum UV to IR. AR coating and protective layer coating are also ...
It has a very high 1.25 numerical aperture and a short focal length of 0.2mm. At 100x the field of view is only 0.2mm wide.
VIS-NIR: Our visible/near-infrared broadband anti-reflection coating is specially optimized to yield maximum transmission (>99%) in the near-infrared.
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Wavelength influences the diffraction of sound waves just as it influences the diffraction of light waves. The ability of a sound wave to diffract decreases as ...
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