Lens centering error: 3~5'. Waveband: 400-1100nm. Surface finish: Grade 3-4.

You can find the formula there or on Wikipedia Gaussian function - Wikipedia And the calculation of the FWHM is rather simple to derive based on the one from the regular exponential,

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Wide-angle Lens—FX format approx. 14 – 35mm / DX format approx. 10 – 24mmWide angle lenses are the popular choice for landscapes, interiors, large group photos and when working in confined situations.

I’m curious if anyone has insights as to whether FWHM (full width half max) can be applied to linear objects for length measurement. I understand that FWHM is typically applied to spherical objects. However, I’m unclear if it can also be applied to linear objects.

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If you want to draw these manually and then assess their distance, @dnmason’s approach is on the right track, except that the FWHM can be defined for functions other than the plain gaussian. In the case of these line-like objects and distributions, a good candidate fit is the supergaussian. Our first dealing with the supergaussian with my colleague @romainGuiet stemmed from a project a couple of years ago that led us to this paper and led us to try it out on ImageJ’s Curve Fitter.

Super Telephoto Lens—FX format approx. 300 – 600mm / DX format approx. 200 – 600mmThese lenses provide a good range for sports and wildlife photography where the photographer is limited as to how close they can get to the subject.

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There are two types of lenses-prime and zoom. Prime lenses have a fixed focal length and zoom lenses have variable focal lengths. The advantage of the zoom lens is its versatility. They are ideal when you are photographing a variety of subjects such as landscapes and portraits, and you just want one lens for both situations. Using a zoom lens also reduces the number of times you need to change the lens which saves time and limits the possibility of getting dust in the camera's mirror box or on the sensor.

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Axicons are conical-shaped optical components used to create a ring-shaped beam.

I’m trying to use this macro to measure the FWHM of collagen fibers in second harmonic generation images, but I get an initialization failure when I launch the macro. I adapted Romain Guiet’s macro to work with 2D images and it works nicely, but I would like to try the above macro too. Can someone please with the macro code? I’ve attached an example image. Thank you for your help! Slide_13_skin_ctrl_1.5x - DenoisedCropCLAHE2.tif (6.4 MB)

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I’ve attached a macro that takes a line profile and tries to fit a supergaussian through the data and provides the FWHM. Hopefully this can be useful as a sarting point as well as the other approaches suggested.

The focal length tells us the angle of view—how much of the scene will be captured—and the magnification—how large individual elements will be. The longer the focal length, the narrower the angle of view and the higher the magnification. The shorter the focal length, the wider the angle of view and the lower the magnification.

Macro Lens—FX format 60mm, 105mm, and 200mm / DX format 85mmClose-up photography uses a specific range of lenses that allow up to 1:1 reproduction. These lenses allow the photographer to focus very close to the subject and reproduce them at a 1:1 life-size ratio on film or an imaging sensor. These lenses are popular for subjects such as flowers, insects and small products.

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Telephoto Lens—FX format approx. 70 – 200mm / DX format approx. 55 – 200mmTelephoto lenses between 70 – 200mm are very popular for portraiture and product photography as well as nature and wildlife imagery. They allow the photographer to produce close crops on the subject. In the case of portraiture a telephoto allows the photographer to take the photo at a distance that does not intrude upon the subject.

@WJ89 the FWHM can be applied as long as you consider that drawing a line trough the half maximum of a profile and measuring its length (or infering it from calculation) is meaningful. With smooth constant lines like the one @dnmason showed, this can be useful, but might redirect the problem to “How do we get to draw a line profile across the object in the first place?”

Note that the curve fit of the line is way off because the intensity of the line plateaus along the length of the line. Depending upon the length of the line you will either under or overestimate if you calculate a FWHM.

Prime lenses also tend to have a larger maximum aperture (f/1.4 to f/2.8). This is an advantage when shooting in low light conditions as it will increase the possibility of hand holding the camera and freezing the subject without shake or blur caused by the longer exposures. Photographing using prime lenses with large apertures also means you can get a shallow depth of field which is useful for portraiture where you might want a softer or blurred background (also known as bokeh).

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The main advantages of prime or fixed focal length lenses are their size and weight as well as their maximum aperture or f/stop. Prime lenses tend to be more compact and lightweight than zoom lenses.

I tried DiameterJ, but I keep getting macro errors, even when I use the macro in an old version of FIJI, so Local Thickness will definitely work here. Thank you ever so much!

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Extension distortion, also called wide-angle distortion, is when objects close to the camera appear unnaturally large (or far apart). This effect is especially ...

The simplified and short answer (in my opinion) is no. Here’s the thing: a FWHM analysis relies upon a profile which is roughly Gaussian. For example, take the following image:

Roughness profile. The profile derived from the primary profile by suppressing the long wave component using the high-pass filter with a cutoff value of λc. The ...

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You can draw horizontal line selections through the two objects, plot the profiles ( Analyze > Plot Profiles ) then curve fit them with an offset Gaussian ( Analyze > Tools > Curve Fitting ) and you get this:

Apr 27, 2022 — Designed for a simple screw mount and a short flange distance (17.5mm), C-Mount lenses are perfect candidates for adapting onto mirrorless ...

Focal length, usually represented in millimeters (mm), is the basic description of a photographic lens. It is not a measurement of the actual length of a lens, but a calculation of an optical distance from the point where light rays converge to form a sharp image of an object to the digital sensor or 35mm film at the focal plane in the camera.

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Standard Lens—FX format approx. 50 – 60mm / DX format approx. 35mmStandard lenses are popular as they are closest to the angle of view we humans see. These lenses have minimal distortion, which can be flattering to the subject. They tend to use large apertures and allow a lot of light to enter the lens which makes them fast in low light conditions. The large apertures (f/1.8 – f/1.4) also produce a pleasing out-of-focus effect to the background which concentrates the attention of the viewer on the subject. Standard lenses are the popular choice for a wide range of photography including portraiture, nature and low light situations where the photographer can not use a flash or is looking to capture the scene with available light.

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Indeed there seems to be something off. I managed to get it working again, and I tried a new formulation that give the FWHM directly.

The Local Thickness plugin is a terrific suggestion. I’m mad at myself for not thinking of it. First I thresholded with the Huang algorithm, made a mask, despeckled, and used Analyze Particles to get rid of small bits, then I used Local Thickness and got this result. image1920×1218 304 KB

It’s hard to say without seeing an example image, but you may want to try creating a binary mask then running Skeletonisation followed by running Analyze skeleton which will give you a line length as well as some branching parameters.