For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

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For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

At a basic level, then, you should note that 395 nm is about 30 nm closer to visible light (violet) than 365 nm is. Or, in other words, 365 nm is "deeper" into the UV spectrum than 395 nm is.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

Nikon40xobjective

The main difference is that the 395nm LED emits much more visible light than the 365nm LED. The 395nm LED emits a pronounced violet-colored light, while the 365nm LED emits a dull, bluish-white light (the result of residual light energy that "tails" off into the visible spectrum). Both types emit in the UVA wavelength range and are generally capable of producing "blacklight" or curing effects.

Polarization incoherence examples · P polarized light will have higher transmission (low reflection) · S polarized light will have lower transmission (high ...

Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

Both 365nm and 395nm options are within the UVA wavelength range. Generally speaking, UVA lights are useful for creating and observing fluorescence effects, as well as for plastics and paint curing. UVA wavelengths are safer when compared to stronger UVB and UVC rays.

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

Of course, the 395nm LED also emits at wavelengths below 395nm as well, and the light energy at these wavelengths are very effective at creating fluorescence effects or initiating UVA reactions. But, as the chart shows, it is important to notice that a significant portion of the light energy is being emitted in the visible, violet region.

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For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

Nikon60Xobjective

To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

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Conversely, looking at the 365nm spectral output, you will notice that virtually all of the light energy is within the invisible UVA range only, with emission energy tailing off before reaching 400nm. This will maximize the amount of UVA energy compared to visible light energy and is preferred for most UVA applications.

For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

Nikon objectivethread

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

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In addition to 365nm UV lights having the advantage of not emitting violet light, many objects will fluoresce strongest at 365nm, as evidenced by absorption spectrum measurements.

Nikon100xObjective

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

Even though lights at wavelengths below 400nm are not visible to the human eye, the wavelength value is still nonetheless very important. For example, 365nm wavelength UV belongs to a type of ultraviolet known as "UVA," while 290nm wavelength UV is considered "UVB." The wavelength is what determines the type of UV, and this is an important distinction among UV categories in terms of application and even safety precautions that are necessary.

Sep 20, 2018 — The numerical aperture of a microscope objective is a measure of its ability to gather light and resolve fine specimen detail at a fixed object ...

Due to the way UV LED diodes are manufactured, 365nm wavelength chips tend to be less efficient and more costly. The exact difference in price will depend on the product type, but in general, 365nm UV LEDs do come with a price premium, and as such, 395nm UV LED products are better suited for those on a budget.

The result is that 365nm light may be better suited for applications where stronger fluorescence effects are desired. Coupled with the advantage of having a lower amount of visible, violet light emitted, 365nm light can be considered an optimal choice where performance is of paramount importance.

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

Nikon objectivefield number

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

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Normal Distribution X∼N(μ,σ) ; μ= σ= ; x=.

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

Nikon20xObjective

For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

If you're looking for some UVA lights, you may come across two specifications - 365nm or 395nm. What does that mean, and which one is best? Read on to find out more!

When illuminating a 365nm LED, however, you will likely notice a dull, bluish-white color emitted. This is caused by visible light "leakage" where minuscule but visible amounts of visible wavelength energy (i.e. white light) are also emitted. The proportion of this visible light energy is so small that it is shown as "0" in the spectral charts, but in some applications such as UV photography, the visible light is indeed detectable and may be a nuisance. In such cases, additional visible light filtering techniques may be necessary.

Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

OlympusobjectiveFinder

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

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These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

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As you may recall, different wavelengths of visible light appear as different colors to us. 450 nanometer light, for example, is a royal blue color, while 630nm is a punchy, red color. The light that has a wavelength between 400nm and 800nm is visible, while light that falls below 400nm is considered invisible, ultraviolet light.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

The 395nm LED emits strongest at 395nm (as defined by the term peak wavelength), but it also emits quite a bit of energy at 400nm, and even 410nm. These wavelengths are solidly in the visible, violet portion of the spectrum.

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

Nikon objective selectorapp

Why is there a difference in the amount of visible light? The chart above shows the spectral output diagram of both the 365nm (narrow dotted line) and 395nm (wide dotted line) LEDs. What you will notice is that both 365nm and 395nm LEDs emit over a range of wavelengths both above and below their respective wavelengths. In other words, 395nm LEDs don't emit at only 395nm.

Before we get into answering the question of whether 365nm or 395nm is better for your application, it is important to understand the basic principles of wavelength and light.