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What is the light source on amicroscopeused for
Our systems are dedicated to enhancing data generation and interpretation for researchers and clinicians through generating bright, high-contrast images. Unlike standard LEDs, our unique active cooling technology and expert LED assembly enables irradiance levels up to five times greater than other LED microscope lighting options. Thermal management holds the LEDs at a constant temperature, allowing us to drive them more powerfully. This increases irradiance, stability, reliability and lifetime, while offering the lowest energy consumption on the market. CoolLED is also the only company to provide direct fit products as an alternative to light delivery with a liquid light guide. This is the most efficient means of light delivery to a microscope and is our most popular type of LED microscope lighting solution. It harnesses more power, while also reducing total cost of ownership thanks to fewer consumables.
Since 2006 when our team of four launched the first commercially available LED illumination system, we have led the way in transforming light source technology. We cover fluorescence microscopy and transmitted light applications, backed by our technical know-how and unique blend of optical engineering and life science expertise.
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LED Microscope Lighting Safety and the Environment: There is no denying that mercury is hazardous, unlike LEDs. LEDs are also more energy efficient, emit much less heat and are in many ways better for the environment.
LEDMicroscopelight source
5. Once the flatness measurement has been completed, lift (do not slide) the optical flat off the component and return it to its protective box.
LED Microscope Lighting Control: Unlike mercury and metal halide lamps which require mechanical shutters, electronic control of LEDs is simple and fast. The ability to switch them on and off with precise microsecond timing is perfect for capturing high-speed events, and electronic modulation of irradiance means no more neutral density filters.
LED Microscope Lighting Performance: Being solid-state, LEDs are much more stable over time, leading to a robust level of irradiance and highly repeatable experiments.
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Microscopering light
4. Place the optical flat over the area that is to be measured. Do not slide the optical flat as this will generate scratches. Apply light finger pressure to the optical flat to remove as much air as possible from between the component and the optical flat. This will usually make the light bands easier to read.
LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.
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If you’re not sure whether a CoolLED Illumination System is right for you, we can provide a free loan unit for testing, with no obligation to purchase. Please contact us at [email protected] for more information.
Microscope lightingled
Produces clear flatness readings when used with Kemet Optical Flats. The compact designed Light is easily transportable and uses a sodium long-life sodium light source. Supplied with a flatness reading interpretation chart.
At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.
This illustration shows how a human hair compares in size to a 1 micron particle. You will not see particles that are 1 micron in size, but if present, these can affect the flatness reading by as much as 3 light bands. This highlights the need for perfectly clean components and optical flats. When cleaning the optical flats, use a lens grade cleaning tissue and either Isopropyl Alcohol or Kemet CO42 cleaning fluid. Harsh tissues will cause scratches on the optical flat.
MicroscopeLight Bulb replacement
We have a record of >99% on-time delivery in full against acknowledged delivery dates and we pride ourselves on friendly and expert advice whenever it is needed. You will also be covered by our market-leading warranty, which includes three years as standard for all products and a 12 month in-for-out swap if we cannot fix an issue.
The chart above shows typical flatness readings. These black lines simulate the black line on the Monochromatic light lamp cover. This is used only when the light band patterns are curved. For other light band patterns refer to the chart above. The same chart is supplied with the Kemet Monochromatic Light.
LED Microscope Lighting Convenience: LEDs are long-lived and don’t require tricky alignment. Once you switch them on they are ready to be used.
1. It is critical that both the component and optical flat are perfectly clean before inspection. Any amount of contamination, regardless how small, will affect the measurement. 1 sodium light band is equal to 0.29 microns, so any particles or smears on the surface of either the component or the optical flat will affect the measurement.
LED lighting systems are available for fluorescence microscopy and transmitted light applications. Take a look at our product range to discover which system is best suited to your requirements.
The black line on the monochromatic light lamp cover should be reflected on the surface of the optical flat. The number of light bands that this black line passes through will give the flatness reading if the bands are straight or curved.
Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?
2. The Kemet Monochromatic Light should be turned on at the start of every shift. The sodium bulb will take up to 15 minutes before it is ready to be used for inspection. The light should be orange in colour when ready.
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Microscope lightingkit
A range of LED lighting products are available with a variety of LEDs and different levels of control. Single wavelength models are ideal for screening applications, and simple white light options are the perfect replacement for a mercury/metal halide lamp. For controllable, everyday fluorescence, the pE-300 Series offers three wavelengths covering all the common fluorophores. Our range extends to the high-end pE-4000 LED microscope lighting solution which offers 16 wavelengths, spanning 365 nm-770 nm. This can excite in the UV region and right through to fluorophores at the IR end of the spectrum (which is ideal for imaging thick samples while protecting against phototoxicity). This even opens up the development of new fluorophores which no longer depend on the emission spectrum of a mercury/metal halide lamp.
LEDs and LED microscope lighting have come a long way since they were first developed for microscopy in 2006. The wealth of benefits compared to traditional microscope lighting such as mercury and metal halide lamps are driving more and more scientists towards this efficient, highly controllable technology.
Whether using fluorescence or transmitted light, or a compound or stereomicroscope, high-quality science begins with high-quality microscope components – including the light source.
CoolLED is a fast-growing organisation based in the UK. We draw on the latest technology to design and manufacture a vast range of cutting-edge LED microscope lighting solutions for researchers and clinicians.
CoolLED LED lighting systems are renowned for being robust and reliable. In fact, we recently heard from a customer who purchased one of our first ever products on the market, and who told us: “Our precisExcite is working in a fantastic manner for over 10 years. It has already contributed to more than 10 publications from the lab and will continue to do so.” – Charles Harata, Associate Professor, Department of Molecular Physiology & Biophysics at the University of Iowa
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3. Position the part so that it is under the monochromatic light ensuring that the entire surface to be measured is within range of the light.
For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.
LED Microscope Lighting Cost: In addition to saving on components, the longer lifetime of LEDs is cheaper in the long-term. You also avoid specialist disposal charges due to the high-pressure lamp and mercury content. Take a look at this study by Green Light Laboratories.
We also understand that something out of the ordinary is sometimes required, and manufacturers wishing to gain a competitive advantage can now access our world-renowned LED technology in customised configurations at www.OEMIllumination.com.
But if things do occasionally go wrong, we are there to offer local support through our global network of resellers and Global Sales Team, which means there is always an expert available to help.
Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.