Diffraction Limited Photography: Pixel Size, Aperture and ... - diffraction limit of light
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In a fluorescence microscope, the excitation filter is part of an optical filter set that also includes a dichroic mirror and an emission filter. This setup is crucial for the efficient separation of excitation and emission light, allowing for clear and specific detection of fluorescence.
The excitation filter plays a crucial role in fluorescence microscopy and other fluorescence-based applications. Its primary function is to selectively allow light of a specific wavelength or range of wavelengths to pass through, while blocking other wavelengths. This selective transmission is essential for the excitation of fluorescent materials or dyes.
Overall, the excitation filter is vital for the specificity and efficiency of fluorescence-based techniques, enabling precise visualization and analysis of fluorescent samples.