This 50X microscope objective is specifically designed to correct the NUV (Near Ultraviolet) or 355nm band, catering to the needs of near-ultraviolet lasers. With an NA of 0.65, it manages to attain an extended working distance of 10mm, providing greater flexibility for applications in industrial processing. Achieving a long working distance typically involves utilizing the front set of lenses to widen the light angle, followed by light convergence through the back set of lenses. Micro objectives with high NA and long working distances often require the use of specialized glass, resulting in a more intricate structure compared to conventional microscope objectives

This 50X infinite conjugated long working distance microscope objective is a widely used lens applicable in various fields such as biomedical, precision testing, sample observation, and more. Its high magnification capability allows for clear visualization and high-resolution observation of extremely small-sized cells, their biological structures, and the internal structures of precision materials.

Field of viewmicroscope

To find the power of each meridian in any cylinder lens, simply compare the sphere power and the combined power of the sphere and cylinder together. At the axis, there is only sphere power; 90 degrees from the axis, lens power is the sum of the sphere and cylinder values. For example:

This 50X microscope objective is meticulously engineered to deliver superior performance with its elevated numerical aperture (NA) and expansive field of view, which is complemented by an extended working distance of 10mm—equivalent to 2.5 times the focal length. Designed to operate within the wavelength range of 355-532nm, it spans the near-ultraviolet band, making it exceptionally well-suited for precision laser processing at 355nm and 532nm. Remarkably, it also demonstrates impressive imaging capabilities at 365nm and 405nm. As a quintessential example of a high-end specialized objective, this lens stands out for its unique features, catering to specific and demanding applications.

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A noteworthy feature of this lens is its coverage of the 355nm wavelength in the NUV band. Calibrating in the NUV band poses challenges due to limited material options. Despite the complexity and cost involved, this design addresses the 355nm wavelength, achieving 50% transmittance and excellent achromatic correction.

Lens powers are separated into two principal meridians, the axis and 90° from the axis. Illustration A shows cylinder axis location when looking at the spectacle wearer.

AXIS AND EYEWEAR SELECTION The lens axis describes the major or principal meridians of the prescription, the meridian of greatest and least power; thickest edge of a minus lens and thinnest edge of a plus.

For a +3.00 -2.00D x 045 lens, the two lens powers are +3.00D and the combination of +3.00D and -2.00D or +1.00D; so the power in the two meridians is +3.00D@045 and +1.00@135. A +2.25 sphere lens has the same power at all axes on the lens.

Microscopyu

In this lens, the two powers are -0.50D and the combination of -0.50D and -2.50D or -3.00D. This lens would have a -0.50D@090 and -3.00D@180. While a new dispenser might think that there is little power in these lenses because of the -0.50D sphere power, due to the axis the lenses are really -3.00D@180. This will significantly impact visible edge thickness.

The precise evaluation of microscopic objective performance depends on the accurate measurement of the dispersion spot radius. In this model, the RMS radius of the dispersion spot is designed to be 0.205μm (on the axis), 0.251μm (at 0.7 field of view), and 0.333μm (at 1 field of view). The MTF on the axis meets 2000lp/mm, achieving the diffraction limit. Although a slight reduction in MTF occurs off the axis due to minimal astigmatism and chromatic aberration, it still meets 1500 lp/mm, approaching the diffraction limit. When used with a single-point light source, the entire field of view can meet the diffraction limit.

A LENS FORMULA The prescription, or lens formula, is the starting point for picking the best lens material, frames, and eye and bridge size. The right choices will turn that lens formula into comfortable, attractive and well-performing eyewear. Topnotch dispensers see the final eyewear design in every lens formula.

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Patients do not easily tolerate vertical prism differences, often called vertical imbalance. Individual tolerance varies, but a difference between the right and left lens of one prism diopter (1Δ) or more of vertical prism can cause asthenopia, (eye strain) adaptation problems, reading difficulties and even diplopia (double vision). Patients with single-vision lenses and vertical prism imbalance usually learn to turn their heads to read through the lenses at a point closer to the optical centers. Prism imbalance tolerances of 2/3Δ horizontally and 1/3Δ vertically are generally acceptable. For patients with greater vertical prism imbalance who must look down to read, slab-off design or reading lenses should be considered.

Designed to meet diverse needs, this objective lens provides precise and detailed observation capabilities across different domains. The infinite conjugate design enhances flexibility, enabling compatibility with various microscopy systems and accessories to meet different experimental and observational requirements. This powerful and versatile tool serves professionals in fields such as biology research, medical diagnostics, and materials science, and contributes to advancements in scientific research and technological development.

Parfocal length

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This lens is compatible with eyepieces that feature a 24mm field number and a 0.48mm object field of view. Additionally, the field curvature is within the depth of focus, ensuring consistent clarity across the entire field of view and meeting the flat field requirements for microscopic objectives.

PRISM Prism is required when the line of sight must change direction. A prescription or lens formula with prism, specifies the amount and the direction of the base (thickest part) of the prism. The amount of prism is usually, but not always, the same in both eyes.