I find that the resolving power of the film is most limiting, and that just about any lens - if properly focused - will generate an image in the film plane beyond the capabilities of the film to record.

Cy5color

This plan only provides a guide, please modify it to meet your specific needs.1. Solution preparation(1) Working solution: Dissolve CY5 solid in DMSO to a final concentration of 10 mM and filter sterilize.Note: The working solution must be prepared and used immediately. Before use, 500μg/mL condensation solution (Cat. No.: GA11126) must be used for activation before subsequent labeling experiments can be performed. 2. Experimental steps for labeling proteins with CY5(1) Protein preparation: Dissolve the protein in a pH 8.5 buffer without primary amines (such as Tris or glycine) and ammonium ions, and prepare the concentration to 2-10 mg/mL to obtain the best labeling efficiency. If the pH is lower than 8.0, adjust with 1 M sodium bicarbonate.(2) Calculation of the amount of CY5 working solution: The amount of CY5 (MW=656.81) required for the labeling reaction depends on the amount of protein to be labeled. The optimal molar ratio of CY dye to protein is about 10:1.(3) Labeling reaction: Take the calculated volume of CY5 working solution and slowly add it to the protein sample solution, gently shake to mix, and then briefly centrifuge to collect the sample at the bottom of the reaction tube. Avoid violent mixing to prevent the protein sample from denaturing and inactivating.(4) Incubation reaction: Place the reaction tube in a dark place and gently shake and incubate for 60 minutes at room temperature. Every 10-15 minutes, gently invert the reaction tube several times to fully mix the two reactants and improve the labeling efficiency. Note: (1)This product is an unactivated fluorescent dye. To use this product to label biological molecules such as peptides and proteins, it must first be activated with carboxylic acid. If an activated form is required, sulfo-Cyanine5 NHS Ester (Cat. No.: GC59174) is recommended.(2)This protocol provides guidance for using CY5 for protein labeling experiments. It can be adjusted according to other literature and specific experimental requirements. The operation should be carried out in a sterile environment to prevent contaminants from interfering with the reaction. Avoid direct contact with the reaction reagents.

In light of this discovery, I've foregone using film, and now just view aerial images of my subjects, using a small microscope focused on the film plane.

Cy5Structure

It was a line/mm test, such as the one you refer to. I4ve tried both Soligors(which I believe is not made anymore) and Leicas and I4ve noticed a big difference in performance between the two. If you4re satisfied with how Pentax lenses perform, use them!

Construction and characterization of the FEMMAN chip. e, Fluorescence images of Cy5 on pGOLD substrate at (1) 200 pM, (2) 20 pM, (3) 2 pM, and (4) 200 fM compared to a quartz substrate at (5) 200 pM and (6) 20 pM. The Cy5 fluorophore density at 20 pM on both the (7) plasmonic substrate and (8) the quartz substrate was comparable. Cy5 (Glpbio) labelled primer. The reagents and DNA chip were stored in room temperature for 0 day, 7 days, 14 days, 21 days, and 30 days. Nat Biomed Eng 7.12 (2023): 1636-1648. PMID: 37735541 IF: 28.1

Well, my 200 mm tests at 60 lp/mm at f22. That is from a chart with T-max 100 film. The rest(centers only) f5.6-72, f8-68, f11-72, f16-72 and f22-60. I don't understand the 68 at f8, as I got it on two separate negatives. I must have been reading the negatives late at night!! F11 is the "sweet spot"

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Texas Redexcitation emission

References:[1] Ferri G L, Isola J, Berger P, et al. Direct eye visualization of Cy5 fluorescence for immunocytochemistry and in situ hybridization[J]. Journal of Histochemistry & Cytochemistry, 2000, 48(3): 437-444.[2] Yuan L, Lin W, Zheng K, et al. Far-red to near infrared analyte-responsive fluorescent probes based on organic fluorophore platforms for fluorescence imaging[J]. Chemical Society Reviews, 2013, 42(2): 622-661.[3] Gerowska M, Hall L, Richardson J, et al. Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis[J]. Tetrahedron, 2012, 68(3): 857-864.[4] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes[J]. Nucleic Acids Research, 1994, 22(15): 3226-3232.[5] Ntziachristos V, Schellenberger E A, Ripoll J, et al. Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V–Cy5. 5 conjugate[J]. Proceedings of the National Academy of Sciences, 2004, 101(33): 12294-12299.

-The resolution of negative films both color and B/W varies greatly with exposure. I have yet to see a photodo.com or any other test group take this into account.

Cy3excitation emission

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Cy5spectrum

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Cy5vs Alexa 647

Note: 1. Please make sure the liquid is clear before adding the next solvent. 2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving. 3. All of the above co-solvents are available for purchase on the GlpBio website.

One more thought on testing lenses. The three p67 lense that I own, the 55, 105 and 200 are are all excellent EXCEPT for the 105 at f16 and f22. My 105 goes really soft at 16 and 22. Others tell me that they have not seen this. Point, MTF test published are for that particular sample and MF lenses do seem to vary from sample to sample. Thus doing testing on your own lenses, reveals the individual quirks.

Cy5absorbance

-Most square film shooters crop their final prints to 645. As a custom printer that's produced a LOT of custom enlargements over the years I'd easily put virtually any 6x7 format image ahead of any 6x6 format one for this reason. Yes, color rendition levels out at about 16x20, but raw enlargement factors are still relevant. My older Mamiya type C lenses are easily inferior to Zeiss, but no question that my 16x20 and larger prints are superior to the Hassie ones. Raw surface area defeats lens resolution every time.

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

As long as analog film is being used as a reference source MTF tests are relative at best. And yes, I agree that MTF values are better than LP/MM tests. Some things to ponder;

Cy7excitation emission

But I have seen tests that placed Leicas Summicron 35mm in second place after a low budget lens, a Soligor! Who can believe in that?

CY5 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 650/670nm, respectively[1]. The basic structure of CY5 is a cyclic compound with a conjugated double bond system. It has excellent fluorescence properties in the far-red region and good photostability[2]. CY5 usually exists in the form of its active ester or azide, which enables it to be labeled with biological molecules such as proteins and nucleic acids through covalent bonds[3]. The optimal molar ratio of CY5 to protein is about 10:1[4]. CY5 has a wide range of applications. It is not only suitable for biomarkers and cell imaging, but also for nucleic acid detection, protein analysis, and drug screening[5]. The molecular weight of this product is 656.81. It is for research purposes only and is not suitable for food, drugs, medical devices, or cosmetics.

Bench tests usung extreme conditions (high contrast trans-illuminated targets etc.) are fine if you are trying to show your lenses are

factor. For example an APO-Rodagon 50/2.8 at it's specified optimum aperture (f5.6) and enlargement ratio (10x)has an MTF of about 40% at 80 lp/mm. This means that the contrast of detail at 80 lp/mm in the print will only be 40% of that in the negative! This means that if you can just resolve 80 lp/mm

I think that lens tests might do the work in some cases, but I believe more in what I see with my own eyes, in practical tests. Everyone who has tried a Leica knows that their lenses are of a very high standard, perhaps the best 35 mm lenses on the market.

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CY5 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 650/670nm, respectively.