The video shows the early development of a zebrafish embryo imaged by a light sheet microscope. The time in the left upper corner shows the hours and minutes post fertilization.

Light sheet microscopy

Rory M Power, and Jan Huisken. “A Guide to Light-Sheet Fluorescence Microscopy for Multiscale Imaging.” Nature Methods, vol. 14, no. 4, 2017, p. 360.

Light sheet fluorescence microscopyPDF

Emmanuel G Reynaud, et al. “Guide to Light-Sheet Microscopy for Adventurous Biologists.” Nature Methods, vol. 12, no. 1, 2014, p. 30.

Image

LSFM (Light Sheet Fluorescent Microscopy), also known as SPIM (Selective Plane Illumination Microscopy), is a method of imaging that uses a light sheet to illuminate a sample from the side in the focal plane of the detection objective (Fig. 1).