Eyepiece on a microscopediagram

With the eyepiece reticle installed and a stage micrometer under the microscope, focus so that both scales are cleary in focus and lined up at the "0" point. You will want to calibrate for each objective that you plan to use to make measurements.

Field of View - the diameter of the circle of view that you see when looking through the microscope. When calibrating an eyepiece reticle it is best to use a stage micrometer where most if not all of the ruler on the stage micrometer fits into the field of view. The higher your magnification, the smaller your field of view will be.

Eyepiece on a microscopemeaning

Even though you may be using two identical microscopes - let's say each with 10x eyepieces and a 40x objective, they can stil have slightly different magnification factors. Therefore, it is important to calibrate your eyepiece reticle with a stage micrometer before making measurements with your eyepiece reticle. This ensures that you will be making accurate measurements with your microscope.

Objective lensmicroscope

Eyepiece Reticle (or reticule) -a small piece of glass with a ruler etched into it that fits into a microscope eyepiece. This ruler is used to make measurements of objects viewed through the microscope. The image from the eyepiece reticle is imposed upon the image when looking through the microscope. There are many variations of eyepiece reticles including lines, cross-lines, grids, counting reticles, etc. Before purchasing an eyepiece reticle you would want to make sure your microscope eyepiece accepts a reticle, and you would want to determine the diameter of the reticle that fits in your eyepiece.

Please note that the information we provide is not intended to replace consultation with a qualified healthcare professional. We encourage you to inform your physician of changes in your lifestyle and discuss these changes with your physician. If you have questions or concerns about any health condition you may have, please contact your physician. The statements on this website have not been evaluated by the Food and Drug Administration. The products are not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, please consult your physician before using our products. The content of the website and the products sold are based on the opinion of the author and are provided on an "AS IS" and "AS AVAILABLE" basis only. You should conduct your research and confirm information with other sources when seeking information regarding health issues and always review information carefully with your professional healthcare provider before using any protocols presented on this website and in the products sold here.

Even if you’re happy with your purchase but later change your mind, you can still return it as long as you’re within 180 days. Once FitSpreso process your return, they’ll issue a refund to you.

Stage Micrometer -a microscope slide (generally 1" x 3") that has a ruler etched on it. It is either made of glass (for transmitted light) or metal (for reflected light). The stage micrometer is used to calibrate an eyepiece reticle when making measurements with a microscope.

Microscope eyepiecefunction

When we measure everyday items most of us will grab a ruler in order to make a measurement. When using a microscope, generally magnification is higher and a ruler will not work unless you are using a very low power stereo microscope. When using a compound high power microscope an eyepiece reticle is used to make measurements. Most measurements that are made with a compound microscope are between 0.2um to 25mm. It is hard to make measurements above 25mm becasue most eyepieces on a microscope do not have a field of view greater than 25mm.

Detent - when turning the zoom knob on a stereo zoom microscope, detent is a click stop at each of the objective values. Detent is helpful if you need to make measurements. The detent allows the zoom knob to click into set objective values so you know that you are on a precise objective value. Trying to make measurements with a stereo zoom microscope that does not have detent will result in inaccurate measurements.

Eyepiece Graticule (same as reticle) - a specialized piece of glass inserted into the eyepiece of a microscope, featuring a calibrated grid or scale etched onto its surface. This grid allows for precise measurements of objects observed under the microscope. The image provided by the eyepiece graticule is overlaid onto the microscope view, aiding in accurate measurement and analysis. Available in various configurations such as lines, cross-lines, grids, and counting reticles, it is essential to ensure compatibility with the microscope eyepiece and select the appropriate size when considering the purchase of an eyepiece graticule.

The stage micrometer is 1mm long with 100 divisions, which means that each longer division on the stage micrometer = 0.01mm or 10um. If you count the lines on the stage micrometer where the eyepiece micrometer matched up with it at 60, it equals 400um. So we take 400um / 60 eyepiece reticle reading and we can determine that with that specific objective each division on the eyepiece reticle = 6.67um.

Eyepiece on a microscopepicture

When you shop with FitSpresso, you’re protected by our 180-day money-back guarantee. If you’re not satisfied with your purchase for any reason within 180 days of receiving it, you can return the product and request a refund.

When you look through the eyepiece of a microscope that has a reticle installed in it the ruler (or cross-line, grid, whatever is on the reticle) is super-imposed upon your image. This makes it easy to make quick and accurate measurements, as long as you have calibrated your microscope.

Notice on the scale that the stage micrometer and the eyepiece micrometer (reticle) line up almost exactly at 30 on the eyepiece micrometer, but seem to line up more closely at 60. The larger the number on the eyepiece micrometer, the more accurate your calibration will be.