Objectives are categorized into performance classes on the basis of their transmission, chromatic correction, planarity, and others. From the point of view of the International Organization of Standardization (ISO), there are three groups of objective classes differing in quality of chromatic correction.

To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the working distance of objectives and the refractive index of the medium through which the image is collected by the front lens of an objective.

Lumenmicroscope

Manual adjustment of the correction collar requires time and experience, and is challenging when access to the objective is obstructed by additional equipment. The motorized correction of the Leica motCORRTM objectives simplifies adjustment of the correction collar and reduces training effort. Remote control of the Leica motCORRTM quickly adjusts the optics without disturbing the specimen.

What is the purpose of the objective lens in a lightmicroscope

Within the class of apochromats, Leica offers PL APO lenses especially designed to match the highest specifications for confocal scanning (CS). The latest PL APO CS2 series was further improved over the previous CS series. The chromatic correction of the new Leica CS2 objectives is perfect over the whole field of view for precise colocalization of different fluorophores. In addition, numerical aperture and free working distance are pushed to new limits. The design of the Leica CS2 objectives goes hand in hand with the innovative UV optics of the Leica SP8, and the STELLARIS platform to give the most stable UV color correction. For aberration-free imaging in aqueous samples such as living cells, Leica Microsystems has developed a series of superior high-resolution water immersion objectives. For the best imaging results, these objectives require a correction collar to adapt the optics to varying coverglass thickness, changing temperature, and specimen inhomogeneity.

Leica achromats are powerful objectives for standard applications in the visual spectral range, offering field flatness (OFN) up to 25 mm. The absolute value of the focus differences between red wavelength and blue wavelength (2 colors) is ≤ 2x depth of field of the objective.

Leica semi-apochromats are objectives for applications in the visual spectral range with higher specifications, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 2.5x depth of field of the objective.

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Function of the Excitation Filter in a Fluorescence MicroscopeThe excitation filter plays a crucial role in the operation of a fluorescence microscope.

Leica’s Fluotar portfolio features various application-optimized correction collars (CORR) to compensate for external influences such as temperature, coverslip thickness and immersion media.

Pincushion distortion is a type of optical aberration where images appear pinched or bowed inward at the corners, making straight lines look curved. This ...

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Brightness: The microscope objective determines the brightness of illumination, and rises with higher Numerical Aperture (NA) and falls with increasing ...

Apr 24, 2024 — Although these are a bit thicker than Fresnel lenses, they can be positioned much closer to the display with a distance of less than 1 mm. Thus, ...

What does the objective lens do on amicroscope

Leica semi-apochromats are objectives for applications in the visual spectral range with higher specifications, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 2.5x depth of field of the objective.

For research involving highly specialized techniques or challenging samples, specially optimized objectives are often needed. Leica Microsystems offers a wide choice of special objectives for such applications.

Whereas PL APO objectives are corrected in the visible wavelength range, with the PL IRAPO objectives a new set of specialized objectives is now available for improved multiphoton imaging (MP). The IR Apochromats are color corrected from at least 700 nm up to 1300 nm and highly transmissive in the visible and infrared wavelength ranges with > 85 % transmission from 470 – 1200 nm. Thus they are ideal for non-linear imaging like multicolor multiphoton imaging including excitation with OPO (optical parametric oscillator), and CARS (Coherent Anti-Stokes Raman Scattering).

Opticalmicroscope

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For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the eyepieces. Of course, this is assuming that the microscope is already corrected for Koehler Illumination during which the condenser and diaphragms are adjusted.

Confocal microscopy

Leica apochromats are objectives for applications with highest specifications in the visual range and beyond, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 1.0 x depth of field of the objective.

Leica objective

FL PLAN objectives are modern universal objectives with outstanding chromatic correction and field flatness (OFN) up to 25 mm.  State-of-the-art coating techniques give FL PLAN objectives high transmission for fluorescence excitation. All contrasting methods are possible. FL PLAN objectives are optimized for fluorescence, PH and DIC.

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Field planarity (PL) is computed for a 25 mm field of view. They are made of special glass to enable maximum transmission. This makes them powerful photon collectors in fluorescence microscopy.

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The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

PL APO objectives – the class for professionals. They deliver imaging quality that cannot be achieved by conventional objectives. Plan Apochromats provide perfect axial and lateral color matching for applications requiring fast color change and the colocalization of structures. PL APO objectives are characterized by flawless image flatness up to a field number of 25. Top numerical apertures define a resolving power at the limits of what is physically possible.

The optical microscope has been a standard tool in life science as well as material science for more than one and a half centuries now. To use this tool economically and effectively, it helps a lot to understand the basics of optics, especially of those essential components which are part of every microscope.

Zeiss objective

HI PLAN objectives feature good chromatic correction at two wavelengths and flatness over the whole field of view. Even the edges of the image are sharp and require no refocusing. HI PLAN objectives are designed for visual fields up to 25 mm. Versions for phase contrast are available. Leica HI PLAN objectives offer good correction properties while being extremely reasonably priced.

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The N PLAN objective class comprises further improved achromats with good field flatness up to 25 mm. N PLAN objectives are suitable for transmitted light and DIC, N PLAN PH for phase contrast. For incident light applications including differential interference contrast (DIC), N PLAN EPI objectives are available. The N PLAN EPI BD can also be used as a darkfield objective. The N PLAN EPI incident light objectives feature excellent image contrast and safe working distances.

Feb 5, 2023 — The mathematical description of aspheric lenses is based on a surface profile equation that defines the shape of the lens surface. This equation ...

Leica Microsystems has aligned all three performance classes of its range of objectives to the needs of its customers and the specific requirements of a wide variety of applications – while offering a convincing price-performance ratio.

Motiv-Tracking-AF · Drücken J , um die Fokusverfolgung zu aktivieren; Der Fokuspunkt ändert sich in ein Fadenkreuz. · Positionieren Sie das Fadenkreuz über dem ...

Objective lensmicroscope

PL Fluotars are powerful universal objectives with outstanding chromatic correction for at least three wavelengths which makes them suitable for fluorescence imaging.

Leica apochromats are objectives for applications with highest specifications in the visual range and beyond, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 1.0 x depth of field of the objective.

Leica achromats are powerful objectives for standard applications in the visual spectral range, offering field flatness (OFN) up to 25 mm. The absolute value of the focus differences between red wavelength and blue wavelength (2 colors) is ≤ 2x depth of field of the objective.

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