Samples observed under dark-field microscopy should be carefully prepared since dust and other particles also scatter the light and are easily detected. Glass slides need to be thoroughly cleaned of extraneous dust and dirt. It may be necessary to filter sample media (agar, water, saline) to exclude confusing contaminants. Sample materials need to be spread thinly; too much material on the slide creates many overlapping layers and edges, making it difficult to interpret structures.

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Nature photography requires an assortment of lenses depending on the subject. Typically normal to wide angle lenses are used for landscape photos and very long telephotos are used for wildlife. These eight photos were taken from the same place with different lenses. The subject is Longs Peak from Upper Beaver Meadows in Rocky Mountain National Park. 24mm 35mm 50mm 100mm 200mm 400mm 800mm 1200mm "I want to take a bluebird picture. What lens should I use?" It is a symptom of bird photography that your lenses never seem to be long enough. This is because birds are so small. When photographing large animals, a 400mm gives you decent image size from a reasonable distance. But remember, an elk is six or seven feet tall. A bird is barely six inches long, so when your subject is twelve times smaller, you have to be twelve times closer. As you can see, even a huge 800mm lens at the relatively close distance of 20 feet doesn't give the huge magnification you might expect. The field of view is about ten inches. It takes a big lens to photograph a small bird. Home © Copyright David Dahms

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This page titled 3.3A: Dark-Field Microscopy is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless via source content that was edited to the style and standards of the LibreTexts platform.

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You can see that when the camera sensor has lower resolution (meaning larger sensor pixels) there is not a significant difference in the images produced by the ...

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Dark-field microscopy has many applications in microbiology. It allows the visualization of live bacteria, and distinguishes some structure (rods, curved rods, spirals, or cocci) and movement.

24mm 35mm 50mm 100mm 200mm 400mm 800mm 1200mm "I want to take a bluebird picture. What lens should I use?" It is a symptom of bird photography that your lenses never seem to be long enough. This is because birds are so small. When photographing large animals, a 400mm gives you decent image size from a reasonable distance. But remember, an elk is six or seven feet tall. A bird is barely six inches long, so when your subject is twelve times smaller, you have to be twelve times closer. As you can see, even a huge 800mm lens at the relatively close distance of 20 feet doesn't give the huge magnification you might expect. The field of view is about ten inches. It takes a big lens to photograph a small bird. Home © Copyright David Dahms

Darkfield microscopy

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The entire field appears dark when there is no sample on the microscope stage; thus the name dark-field microscopy. When a sample is on the stage, the light at the apex of the cone strikes it. The rays scattered by the sample and captured in the objective lens thus make the image.

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Dark-field microscopy is ideally used to illuminate unstained samples causing them to appear brightly lit against a dark background. This type of microscope contains a special condenser that scatters light and causes it to reflect off the specimen at an angle. Rather than illuminating the sample with a filled cone of light, the condenser is designed to form a hollow cone of light. The light at the apex of the cone is focused at the plane of the specimen; as this light moves past the specimen plane it spreads again into a hollow cone. The objective lens sits in the dark hollow of this cone; although the light travels around and past the objective lens, no rays enter it.

Where do those numbers come from? Lenses are marked with a series of f-stops, each one lets in half as much light as the previous one. The light-gathering ability of a lens is determined by its area, and f-stops are determined by diameter. Area is related to diameter squared. The progression of f-stops, 1 - 1.4 - 2 - 2.8 - 4 - 5.6 - 8 - 11 - 16 - 22 - 32, are powers of the square root of 2. For a further explanation of f-stops, try this. Lens Focal Length Chart Nature photography requires an assortment of lenses depending on the subject. Typically normal to wide angle lenses are used for landscape photos and very long telephotos are used for wildlife. These eight photos were taken from the same place with different lenses. The subject is Longs Peak from Upper Beaver Meadows in Rocky Mountain National Park. 24mm 35mm 50mm 100mm 200mm 400mm 800mm 1200mm "I want to take a bluebird picture. What lens should I use?" It is a symptom of bird photography that your lenses never seem to be long enough. This is because birds are so small. When photographing large animals, a 400mm gives you decent image size from a reasonable distance. But remember, an elk is six or seven feet tall. A bird is barely six inches long, so when your subject is twelve times smaller, you have to be twelve times closer. As you can see, even a huge 800mm lens at the relatively close distance of 20 feet doesn't give the huge magnification you might expect. The field of view is about ten inches. It takes a big lens to photograph a small bird. Home © Copyright David Dahms

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The focal length of a mirror and a lens can be calculated using 1/do + 1/di = 1/f, where do is the object distance, di is the image distance, and f is the focal ...