One other benefit of longer working distance is you have less out of focus areas in a thicker sample. This is known as Depth of Field.

It really depends on your sample. A mostly flat sample can afford a short working distance and good resolution. A sample with a lot of differing heights or peaks and valleys will benefit from a long working distance objective so as not to crash into neighbouring peaks.

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2019527 — The closest focusing distance of a lens (see A below) refers to the shortest distance that must be between your subject and the surface of the ...

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2023927 — In this article, we will explain the pros and cons of each type of lens, and give you some tips on how to use them effectively.

At some point we all have This is where the concept of Working Distance comes in. You will see Working Distance (WD) often listed as a spec in microscope or objective brochures. This spec is important as it tells you the distance between the microscope objective lens and your focused sample.

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