In normal bright-field microscopy, light is focused by an objective lens (characterized by its magnification and numerical aperture (NA), e.g. 50x/NA=0.75) onto the sample and the light either reflected (reflected light microscopy) or transmitted (transmitted light microscopy) by the sample is collected. The image contrast is primarily based on light absorption.

Unpolarized light of intensity I passes through an ideal polarizer A. Another identical polarizer B is placed behind A. The intensity of light beyond B is ...

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Dark fieldscattering spectroscopy

Chromatic aberration, also known as color fringing, is a color distortion that creates an outline of unwanted color along the edges of objects in a photograph.

Jun 30, 2015 — Humans perceive polarised light using Haidinger's brushes, a subtle visual effect which appears like a yellow bow-tie at right angles to the ...

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Dark fieldmicroscopy

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Lightfieldmicroscopy

by RMA Azzam · 2010 · Cited by 4 — Ts is maximum at θ=α/2 for all prism angles α. However, Tp is maximum at θ=α/2 for α≥60° but has a shallow minimum at θ=α/2 for α≤50°. Consequently, MFT for p- ...

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Köhlerillumination

In dark-field microscopy, the inner part of the illuminating light beam is blocked by a mask. Thus, only a sheet of light is focused onto the sample. In other words, the sample is illuminated under an angle that is defined by the NA of the objective lens. Only the fraction of light is detected, which has changed its angle upon interaction with the sample. The image contrast is primarily based on light scattering, enhancing the contrast of, e.g., small particles and sharp edges.

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