If you’re not working with strict tolerances, an accuracy of 0.5% may suffice. However, if you need more accuracy than that, go for a digital microscope measurement software with significantly higher accuracy. As an example, the TAGARNO Ruler app has an accuracy of 0.5%, while the TAGARNO Measurement app has an accuracy of 3µm.

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In conclusion, infinite conjugate long working distance microscope objectives play a pivotal role in various scientific and industrial applications by providing high-resolution imaging capabilities. Their evolution highlights the constant need for advanced design and development to meet diverse research and production demands. Long working distances offer operational flexibility, collision avoidance, and suitability for various sample thicknesses, making them indispensable in critical scenarios like optical fiber alignment, atom trapping, and crystal growth observation.

Below you’ll find two digital microscope measurement software options that are developed by and compatible with selected digital microscopes from TAGARNO. Both options can be used without a separate computer and run on the extremely high quality microscopes from TAGARNO with FHD 1080p camera resolution, 60 frames per second and 30x optical zoom.

You will be able to reap additional benefits depending on how advanced the digital microscope measurement software that you choose is and what your starting point is. For that reason, let’s have a look at what you need to consider when choosing your next digital microscope measurement software and what benefits you can expect depending on what you choose.

Resolution is expressed as a function of wavelength (λ) and numerical aperture (NA), with NA calculated as the product of the refractive index (n) and the sine of the half-angle (θ) of light from the object. Achieving high magnification often involves a trade-off, as increasing NA for better resolution concurrently poses challenges in correcting on-axis and paraxial aberrations and achieving long working distances. For an aberration-corrected objective that reaches the diffraction limit, the resolution is usually expressed as follows:

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For instance, the introduction of a filter between the objective and tube lens permits the observation of specific wavelengths of light or the blocking of unwanted wavelengths that might disrupt the setup. Fluorescence microscopy commonly employs this design. Another advantage of the infinite conjugate configuration is its capability to adjust magnification according to specific application requirements. The objective magnification is determined by the ratio of the tube lens focal length (fTube Lens) to the objective focal length (fObjective). By altering the tube lens focal length, typically a 200mm achromatic lens, the objective magnification can be customized. If an objective follows an infinite conjugate design, the objective body will bear an infinity symbol.

Despite the challenges posed by achieving long working distances, the ongoing advancements in optical design and lens processing capabilities continue to push the boundaries of microscope objective performance, ensuring clear imaging and enhanced resolution for a wide range of applications.

However, achieving long working distances often involves employing a reverse telephoto design structure, which increases lens diameter and complexity. This imposes higher requirements on optical design and lens processing capabilities.

Lastly, a thing worth considering when choosing your digital microscope measurement software is what kind of additional equipment is required to perform your measurements. While some digital microscope measurement software can run directly on the microscope itself, others require that you connect the microscope to a computer and perform the measurement on here.

The evolution of microscope objectives, with a historical backdrop, underscores the ongoing importance of design and development. The demand for specialized non-standard lenses in scientific research and increasing requirements in various fields drive the development of objectives with better flat-field characteristics, chromatic aberration correction, and, notably, long working distances.

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Contrastingly, in an infinite conjugate or infinity-corrected optical system, light originating from an infinite distant source is focused on a small spot. Within an objective, this spot serves as the object under examination, while infinity points toward the eyepiece or camera sensor (refer to Figure 12). This sophisticated design incorporates an additional tube lens between the object and eyepiece, enabling the production of an image. Despite its complexity compared to finite conjugate designs, the infinite conjugate system allows the integration of optical components like filters, polarizers, and beamsplitters into the optical path. This feature facilitates advanced image analysis and extrapolation in complex systems.

Working distance, denoting the distance between the object and the lens’s front end, emerges as a crucial parameter in microscope objective selection. Longer working distances afford greater flexibility in applications. For instance, in optical fiber fusion, a high-magnification objective with a long working distance improves alignment precision by providing ample space for the optical fiber.

Another important factor to consider when choosing digital microscope measurement software is the type of measurements you want to perform. Make sure that the software offers those specific measurement tools. Further, consider any additional features that would be nice to have, either now or at a later point. Such features could include:

Where λ is the band and NA is the numerical aperture of the lens, which is the most commonly used aperture representation method for microscopic objective lenses:

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Unlike other similar measurement software, this one doesn’t require a separate compute. All it needs is a mouse and a keyboard connected to use all the built-in features.

Parfocal length

With the Ruler app, you can activate an on-screen Ruler in a variety of colors and directions and use it as a heads-up display to quickly measure your samples. The Ruler app is factory calibrated and has an accuracy of 0.5%. This combined with the fact that you can save your most used ruler settings as a preset makes the Ruler app a great tool if efficiency, reduced sample preparation time and consistency across inspections are key KPIs for you.

However, we’ve created a list of things to consider if you’re currently looking for a digital microscope measurement software. If you keep these things in mind, we’re confident you’ll be able to find a digital microscope measurement software that meets your needs and requirements.

Even if you have a digital microscope already that offers digital microscope measurement software, make sure to check that the microscope has enough magnification capacity and camera resolution to display your samples in a quality that you can measure on. If the image is pixelated or your sample isn’t magnified enough, it’s difficult to make accurate measurements. This of course also applies if you don’t have a digital microscope already.

Focal length is the distance from the center of the lens to the image sensor (focal plane), and each lens has a different focal length.

Field of view microscope

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Now that we know what digital microscope measurement software is, its benefits and what to consider when choosing the right one for you, let’s have a look at some options.

With digital microscope measurement software, however, the measurement is done on a live or still image from the microscope and a photo of the completed measurement can easily be captured.

If you have a digital microscope already, a great place to start is by considering if there’s any digital microscope measurement software options available for your current microscope. If not, you need to start doing your research by looking for a new digital microscope that offers digital microscope measurement software. This of course makes your research more extensive, but it means you’re in no way limited by your current equipment.

In the optical configuration of a finite conjugate system, light emanating from a light source, not positioned at infinity, converges to a specific spot (see Figure 11). In the context of a microscope, the image of the examined object undergoes magnification and is projected onto the eyepiece or camera sensor. The system’s particular distance is defined by either the DIN or JIS standard, with all finite conjugate microscopes adhering to one of these two standards. This design is prevalent in basic microscopes and finds application in scenarios where cost-effectiveness and simplified design are primary considerations.

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Because of the wide range of digital microscope measurement software available to choose from, knowing which one to choose can be challenging.

The digital microscope measurement software will typically offer a variety of measurement tools, such as point-to-point, radius, angle, and area. With some digital microscope measurement software, you can even highlight interesting areas of an inspected sample and add annotations before saving a photo of it for documentation purposes. This, however, will depend greatly on which digital microscope measurement software you choose.

Microscope objectives are essential components in optical microscopes, serving to magnify and capture images of observed objects. They find applications in diverse fields such as biomedical research, precision detection, and semiconductor processing. Additionally, microscope objectives are employed independently in scientific research and industrial production, for activities such as atom capture and laser processing.

As mentioned previously, digital microscope measurement software needs to be calibrated to perform high quality measurements. This can either be done by the manufacturer or on-site by a microscope operator or supervisor. Which option you choose will typically affect the accuracy of the measurements you’ll be able to perform.

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Digital microscope measurement software is a piece of software for digital microscopes that allows you to perform a variety of measurements on your magnified samples. To perform accurate measurements, the digital microscope measurement software needs to be calibrated. This can either be done by the manufacturer at the time of purchase or on-site by a microscope operator or supervisor.

High magnification means a short focal length, and short focal length can achieve large NA. Increasing NA is a common method to improve the resolution of objective lenses. As a small aberration optical system, a high NA means that more light is collected, but the light must be concentrated in a smaller area. With high NA as the premise, it is not easy to correct the on-axis and paraxial aberrations, and it is even more difficult to achieve long working distances at the same time.

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This not only means that you need to find room for an extra laptop or computer at your workstation. You also need to consider the USB ports available on your microscope and how it will affect the image quality. With USB 2.0 outputs, what is displayed on the computer is a compressed version of what the microscope initially recorded, resulting in a significant loss of detail for your inspections. A USB 3.0, in comparison, can display a non-compressed display format that will not reduce the amount of detail before displaying it on a computer.

The most common reason for choosing a digital microscope measurement software is to eliminate manual measurements, e.g. with calipers. When measuring manually, an operator typically needs to manually note the measurement on a piece of paper or type it into a Data management system. This can be a lengthy process and the accuracy of the measurements will greatly depend on the individual operator and their thoroughness.

A key benefit of using a digital microscope is having access to software that go beyond just magnification and basic digital camera settings. Although there’s many software options to choose from, one of the most used and sought after pieces of software, regardless of industry and sample type, is a digital microscope measurement software that allows companies to measure samples more efficiently and accurately. This can either be done on the microscope itself or on a connected computer depending on which microscope and software you choose.

Working distancemicroscope

Microscope objectives employ two primary imaging modes: finite conjugate imaging and infinite conjugate imaging. Infinite conjugate objectives project images to infinity and necessitate the use of a tube lens for imaging assistance. The parallel light path between the infinite conjugate lens and the tube lens facilitates the incorporation of optical components, such as splitters and polarizers, without compromising imaging quality. This flexibility has made infinite conjugate lenses a mainstream choice in the market.

The biggest difference between the two pieces of digital microscope measurement software is their accuracy and number of features.

Where n is the refractive index of the medium between the lens and the object, and θ is the half-angle of the light from the object. The medium between a conventional lens and an object is air, called a dry lens, and the theoretical limit of NA is 1. If you want to break the theoretical limit, you need to change the medium to water or oil. Long working distance microscope objectives are usually dry lenses.

What is digital microscope measurement software and which one should you choose? Become an expert on the topic by reading this guide.

Apart from operational flexibility, long working distances offer advantages such as collision avoidance between the sample and the objective, suitability for samples of varying thickness, and reduced risks of debris contamination and lens damage during processing. In specific scenarios like atom trapping and crystal growth observation, long working distance objectives become indispensable.

With an accuracy of 0.003%, the Measurement app is a great option if you want to perform more advanced and accurate measurements. The Measurement app offers more than 10 measurement and annotation tools and you can save your measurements as heads-up displays and use them to quickly verify if future samples fall within your tolerances or not.

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For infinite conjugate microscope objectives, magnification is determined by the focal length of the objective and the tube lens. High-magnification objectives, typically exceeding 50X, are associated with enhanced resolution. Resolution, indicating the minimum resolvable distance between two points, is a critical factor in applications requiring detailed imaging, such as optical fiber alignment. The magnification is calculated as follows: