Funeral services for Louise will be held at Smith North Little Rock Funeral Home, 1921 Main Street, North Little Rock on Thursday, September 12th. Visitation will be at 10:00 AM, service at 11:00 am, and committal service will follow at 12:30 PM at Rest Hills Memorial Park.

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Louise was an avid fisherman, gardener, and quilter. She never turned down an opportunity to go fishing, loved working in her garden and created an endless number of beautiful quilts. She made special and unique quilts for each of her grandchildren and great grandchildren.

If you have any questions about microscope contrasting techniques or which microscope provides the best contrast for your application, please contact Microscope World.

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When using a brightfield microscope, thin and transparent samples have a very low contrast and can be barely visible. Phase contrast microscopy is the method of choice for viewing thin, unstained samples such as culture cells. Phase contrast uses a special condenser and objective lenses to enhance the contrast seen in the microscope image. Phase contrast is not useful for thick specimens such as plant or animal tissue sections.

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In interference contrast, previously split partial beams are combined as in phase contrast, interfering with each other as a result. Due to the interference of the two twin images in the intermediate image plane, a light-dark contrast is generated at the edges of objects. By laterally shifting the objective-sided DIC prism, also known as the DIC slider, the contrast can be adjusted in such a way that the light and dark object edges appear on a gray background. As the illumination appears darker on one side and brighter on the other side, a pseudo-relief image is perceived by our brain. This can - but does not have to - match the actual topography of the sample.

Fluorescence is a low-energy form of radiation (emission) that results from a previous high energy illumination (excitation). As soon as the excitation stops, the fluorescence emission stops almost immediately. The particular advantages of fluorescence microscopy include a strong image contrast and its specificity; that is, its ability to specifically detect individual structures down to individual molecules. Fluorescence microscope illuminators often use gas discharge lamps such as HBO, XBO, HXP or long-life LED light sources. LEDs make it possible to change the excitation wavelengths extremely quickly. The downside to LED light sources is that they still exhibit rather low excitation intensity in certain spectral ranges.

When using darkfield microscopy, no light from the illuminator will pass into the imaging system, only light diffracted by the structure is captured by the objective. This is what makes the structures appear rich in contrast, and very bright against a dark background. Darkfield microscopy is especially useful to detect tiny and isolated structural details, such as bacteria. When working with reflected light, darkfield illumination is used to identify grain boundaries in polished and etched metal sections, as well as to detect contaminants and flaws in surfaces.

Hoffman modulation contrast is a form of oblique transmitted light illumination in combination with a grayscale modulator comprising neutral gray absorbing layers mounted in the objective's back focal plane. The modulator consists of three grayscale strips that run vertically through the pupil of the objective. This results in this method's middle gray image background and modulates a relief contrast. iHMC is used in the live-cell microscopy of sperm and egg cells when conducting artificial insemination in human and veterinary medicine.

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Louise Frances Furnell, 102, of North Little Rock, passed away on September 7th, 2024. She was born in Cleburne, Texas to Mike and Julia Dumboski. She was proceeded in death by her husband of 53 years Homer Furnell, son-in-law Jerry Brown, and brothers Chester and Felix Dumboski.

Louise graduated from North Little Rock High School in 1941 and was very active throughout her life. She was an involved member of several groups including Levy United Methodist Church, the Red Hat Society, the Pretty Ladies and the Hays Senior Center where she volunteered over 2,000 hours.

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Louise is survived by her daughter Alice Brown of North Little Rock; sister Mary (Charlie) Simon; her grandchildren Alicia (Cary) Yarbrough, Bruce (Gena) Brown and 5 great grandchildren Devon, Adrianne, Josh Yarbrough and Lyndi and Will Brown.

David Furnell, John Mark Furnell, Danny Dumboski, Larry Dumboski, Jeff Dumboski and Seth Tolliver will serve as pallbearers.

When using a microscope with brightfield, some samples will have a natural contrast that is easily viewed, such as bright plants and flowers, metals, and pigments. Some samples can be stained to increase the microscope contrast. However, low contrast samples such as unstained bacteria, thin tissue slices, live cells, or reflective metal parts require the use of additional microscope contrast techniques in order to view the sample. Here we will explore each of these different microscopy contrasting techniques.

Oblique illumination is recommended to contrast objects that are too thick for alternative contrasting techniques. Oblique illumination directs light beams onto the specimen at different angles. As a result of highly directed one-sided illumination, the sample will have one side that appears bright and the other darker, which results in a shadow-casting relief image showing fine structural details. Oblique illumination microscopy is utilized to view thick tissue samples or solid samples that do not allow light to pass through them, such as automotive parts.

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Polarizing microscopes must be equipped with two crossed polarizers. In most cases at least one polarizer and one analyzer are used. Many materials, like most crystals and some biological structures such as muscle cells, are birefringent, meaning they have two refractive indices. This phenomenon fulfills an important diagnostic function in mineralogy, pharmacology, forensic microscopy, polymer research, or the quality control of textile fibers. Reflected light is used to visualize the contrasts in the origin of structure of opaque metals such as aluminum, zirconium, and others.