I am so thankful to you for the information you provide in this article, but I am a little bit confused with learning Light Microscopes classification as we get informed by Pelczar that Light microscopes are four types. Your content is so helpful for me to give an overview of Electron Microscope but I feel this article has lacks in providing information about Light Microscopes.

some other differences are as follows @ optical microscopes are the simplest and oldest microscope compared to electron microscope @ optical models are cheaper and easier to maintain than electron microscope @ optical microscopes use simple lens whereas EM use an electostatic and electomagnetic lens

Just to let you know, an advantage of a Transmission Electron Microscope over a Light Microscope is that you actually can observe living things, as otherwise detailed in your article. But the rest is great!

The biggest Advantage: they are fun to use, and not complicated. Sample preparation is usually fast and they are suitable for hobby purposes.

One big advantage of light microscopes is the ability to observe living cells. It is possible to observe a wide range of biological activity, such as the uptake of food, cell division and movement. Additionally, it is possible to use in-vivo staining techniques to observe the uptake of colored pigments by the cells. These processes can not be observed in real time using electron microscopes, as the specimen has to be fixed, and completely dehydrated (and is therefore dead). The low cost of optical microscopes makes them useful in a wide range of different areas, such as education, the medical sector or for hobbyists. Generally, optical and electron microscopes have different areas of application and they complement each other.

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Light microscopevs electronmicroscope

Compound microscopes magnify up to about 1000x. The specimen has to be sufficiently thin and bright for the microscope light to pass through. The specimen is mounted on a glass slide. Compound microscopes are not capable of producing a 3D (stereoscopic) view, even if they possess two eye pieces. This is because each one of the eyes receives the same image from the objective. The light beam is simply split in two.

Confocal microscopy

okeyyyy, so, this helped tonsssss with my homework. i was completely clueless ! and then…… i read this and was like “ohhhhhhhh junk ! this shananginz is pretty nippy.” and then my homework was donee ! THANKSS 😀

helped me for my homework but the information was hard to find. Would have better if there was a box showing differences.

The two most common types of microscopes are compound microscopes and stereo microscopes (dissecting microscopes). Stereo microscopes are frequently used to observe larger, opaque specimens. They generally do not magnify as much as compound microscopes (around 40x-70x maximum) but give a truly stereoscopic view. This is because the image delivered to each eye is slightly different. Stereo microscopes do not necessarily require elaborate sample preparation.

Electron microscopes have a range of disadvantages as well: -They are extremely expensive. (false) -Sample preparation is often much more elaborate. It is often necessary to coat the specimen with a very thin layer of metal (such as gold). The metal is able to reflect the electrons. (false) -The sample must be completely dry. This makes it impossible to observe living specimens. (false) -They require more training and experience in identifying artifacts that may have been introduced during the sample preparation process. (false) -The space requirements are high. They may need a whole room. (false)

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Invertedmicroscope

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Electronmicroscope

What if we want to compare the results of images taken by Optical microscope and TEM. I basically need to relate the crystallization in an amorphous region due to the heterogeneous nucleation. Can it be said that the two images show the same feature regarding this?

Thnx alot. It helped me with my biology hw but the information was too much i couldnt summarise all of it or it would take a week

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There are not many things that these two microscope types have in common. Both electron and light microscopes are technical devices which are used for visualizing structures that are too small to see with the unaided eye, and both types have relevant areas of applications in biology and the materials sciences. And this is pretty much it. The method of visualizing the structures is very different. Electron Microscopes use electrons and not photons (light rays) for visualization. The first electron microscope was constructed in 1931, compared to optical microscopes they are a very recent invention. Visit the Microscopy Shop! >>> USA Shop | Germany Shop | UK Shop | Canada Shop <<< As an Amazon Affiliate, I earn a commission but it does not cost you more. >> Read more about different microscopes <<

Dear, I’m a 67 year old man with 6 electron microscopes (only for my hobby). Look http://www.pollendata.be It isn”t expensive at all. Pleasure you can’t measure my friends. It’s time to buy refurbished sems.

Thanks a Million! You made my entire semester by helping me to get started with valuable and informative helpful resourcing.

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im 52 and i own 8 microscopes as a hobby on tuesdays me and my friends love to look at your research to extend our craniums