Field of view microscope10X

Trying to answer this question, we first have to calculate the area of the intermediate image by applying the formula for the circular area A= ¶ x r2 for both cases.

Now it comes to the sample itself. “My sample has a diameter of 30mm, is it possible to see it in total”? With a given FOV, every microscope user is able to work out quite easily the visible sample area with a particular objective.

Magnificationdefinition microscope

This kind of approach is also valid for compound micro- scopes. Motic’s BA410E, combined with the low power objective EC-H PL 2X/0.05 displays a sample area of 22/2 = 11mm Ø, roughly the size of a thumbnail. For pathology this is interesting, but also in botany or zoology this combination may be useful as it represents a “missing link” between macro and micro observation.

Field of view microscope40x

Type of Optics Asphere, Cylinder, Domes/Windows, Large optics (>300mm), Micro optics (<5mm), Molds, Plano, Prism, Prototypes, Transmitted wavefront

In a technical sense, we are talking about the diameter of the INTERMEDIATE IMAGE, created by the objective or, in infinity systems, by the cooperation of objective and tube lens. This intermediate image is enlarged by the eyepiece, acting as a magnifying glass. The labelling of the eyepiece shows the actual FOV in combination with its magnification, e.g. 10X/23 for a standard 10X eyepiece with FOV 23 (1).

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Field of view microscopeformula

Services Assembly, Coating, Electro-mechanical design, Grinding, Lens design, Metrology, Molding, Opto-mechanical design, Polishing, Prototypes, SPDT

Field of view microscope4x

Especially in stereo microscopy, where large samples come as standard, a large FOV is appreciated for a better sample overview before going into details. But how much “more” do I see at a glance in a FOV 23, in comparison with a FOV 20? Is it worth to choose a more expensive model, or is the less expensive solution sufficient?

In addition, Edmund Optics manufactures custom optics, optical components, and optical sub assemeblies for the defense, biomedical and semiconductor industries utilizing innovative manufacturing equipment at its global facilities. Edmund Optics can take your optical project from Design to Prototype to Volume production.

Humans tend to follow the idea “the more the merrier”. Basically this idea is also applied for the FOV. But there are limitations. A FOV 28 may be impressive from the technical side (think about the field flatness), but overburdens the human eye: the eyes have to “roll” in order to cover the whole visible area.

To judge the performance of a microscope, the FIELD of VIEW (FOV) is a criterion of interest. What is this “FIELD of VIEW”?

Depthof field definition microscope

Following this chart, the standard configuration of Motic’s SMZ171 offers an area of 30.7mm Ø at a glance. The total magnification in this case is 7.5X. By adding auxiliary objectives < 1X magnification, this area can be enlarged up to an area of 102mm Ø.

A = area of circular intermediate image (unit: mm2) r = radius of circular intermediate image = 0.5 x FOV A1 = ¶ x r12 A2 = ¶ x r22 A1/A2 =¶x11,5mm2 /¶x(10mm)2 =1,322

The BA410E allows a profound diagnosis in pathology, hematology, and cytology with their requirements for best color fidelity.

This calculation reveals a 32.2% larger intermediate image in the FOV23; 32.2% more information in com- parison to a FOV20 setup.