The origin of the ghost light is not well-documented, but there are several theories surrounding its beginnings. According to theater scholar James Fisher in his book Historical Dictionary of American Theater: Beginnings, the term “ghost light” originated from the era of gas-lit theaters, where dimly lit gaslights were used to alleviate pressure on gas valves.

Every time you use the microscope for transmitted light work, whether brightfield, phase or DIC, you must align the condenser lens to assure Koehler illumination is optimal.  If you fail to do this, you will have poor resolution, wacky contrast artifacts, and unevenly lit pictures.

2013816 — The wavelength of infrared light is longer than red light, in some cases many hundreds of times longer. These longer wavelengths carry less ...

A ghost light is a term to describe a single lamp that is left illuminated on stage when the theater is not in use. A ghost light provides a safety measure by preventing accidents and injuries in the dark and empty theater. The tradition of the ghost light is rooted in the belief that theaters are inhabited by ghosts, and leaving a light on stage is believed to ward off these spirits. It is also seen as a symbol of care and respect within the theater community, signifying that even during periods of downtime, theater personnel look out for one another. The ghost light is typically a low-cost solution to keep the stage minimally illuminated during non-performance times, ensuring the safety of those who may enter the theater.

Opticalmicroscope

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Differential interference contrast microscopy

Zeiss step-by-step video. https://www.youtube.com/watch?v=Qk8dbT-N1xw Cued to where you put the sample on the microscope. If you start earlier, DO NOT touch the lamp housing on any of our scopes as described earlier in the video. You may also ignore the last step of removing the eyepiece to adjust the iris diaphragm. For practical use, it may be set at the numerical aperture of the lens or open fully.

STEP 6 To increase contrast, slowly close the iris diaphram (the aperture inside the condenser) just until the image begins to get dark, then open it a smidge. It should be right on the edge of where it starts to decrease the instensity of the image.

Microscope

Two more highly recommended videos by Jennifer Waters and Ron Vale. Waters' video https://www.youtube.com/watch?v=ZRZ9_ov6Fak goes deep into how Köhler illumination works. Vale's video https://www.youtube.com/watch?v=P1Tb_sllWII is more of a step-by-step on a Nikon microscope.

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TW Cronin · 2011 · 147 — Circular polarization refers to the phenomenon whereby the e-vector of light rotates in a plane, where typically the surface normal indicates the direction of ...

Criticalillumination

First part of this video is accessible and simple. https://www.youtube.com/watch?v=5MfZweoJ6A8 The part at the end about phase contrast is not how we set it up on our scopes.

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When you put your first sample on the microscope, you need to focus on the cells and then align for Köhler illumination.

Aug 23, 2019 — Below we will detail three basic methods for assessing light in your home or office: a quick, somewhat accurate eye test, a somewhat more accurate app that can ...

科勒照明

Be careful when adjusting the condenser diaphragm.  Closing the condenser diaphram reduces resolution.  To maximize both contrast and resolution, close the diaphragm just to the point where the image begins to get dark and no further.   This position is especially important when using Nomarski optics.

A guide to all three types of light management films - light transmission, light diffusion, and light reflection.

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