Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.

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Motivated lighting is a fancy name for any light source that is out-of-frame and also enhances, spreads, or somehow affects the practical light in the scene.

The most obvious place to start is with a dedicated softbox lighting kit. You could also go for an umbrella diffuser — they work great but aren’t quite as adaptable as soft boxes.

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Other options include a white trash bag, a thin T-shirt, or even parchment paper. The different thickness and texture of each item means the quality of your diffused lighting could be hard to predict. But if you’ve already got these things around your house, it’s a cheap way to experiment and see if any of them work with the light sources you want to work with.

Diffused lighting interior design

Light will bounce off most solid objects, which is why any good lighting kit comes with reflectors. But a light diffuser does the opposite — it blocks or reflects part of the light, but still allows enough to properly light a subject or scene.

Let’s pretend you’re filming outdoors, and the noonday sun is creating harsh shadows on your subject. Setting up a sheet overhead doesn’t really make sense, but waiting for a passing cloud would provide a natural diffuser that creates even light distribution.

That wasn’t exactly a textbook definition, but it gets across enough of the basics that you should have an idea of why diffused light matters. Now, we get to the fun part — what it looks like in practice, and what you need to start improving the quality of your lighting gear.

My name is Riccardo, I am from Italy, living in Leipzig since 2012 and currently working in UFZ. I am actively working in the field of image data management and I am Data Steward in NFDI4BIOIMAGE.

Forschungszentrum Jülich is a large-scale national research facility in the Helmholtz Association of German Research Centres. By conducting research on the global challenges currently facing society, we contribute to the welfare of mankind and responsible …

Project Manager for the Helmholtz Transfer Academy "Future Innovators". Interested in anything that can solve problems. Trying to unlock the potential of scientific development to also solve short-term problems of the real-world.

DESY is one of the world’s leading accelerator centres. Researchers use the large-scale facilities at DESY to explore the microcosm in all its variety – from the interactions of tiny elementary particles and the behaviour …

It’s worth investigating any tool or learning any trick to improve the quality of your lighting. And because diffusing light is an easy, cheap, and efficient way to adjust lighting, it’s something that every filmmaker should at least be aware of.

What is transmitted lighting

Diffused light can actually get you the best of both worlds. It’s the process of using a diffuser (which could be a DIY piece or something you bought in a lighting kit) to reduce harsh shadows or the glare of a light source you can’t get rid of on the set.

Bright-field microscopy, a method of microscopy in which the object to be observed is located in front of a bright background, called the bright field, which illuminates the microscopic image. In this process, the light passing through the object (transmitted light microscopy) or the light reflected back from it as a whole (reflected light microscopy) enters the objective of the microscope. Bright-field microscopy requires high-contrast objects, so many transmitted-light objects must be stained. In brightfield microscopy, objects appear dark or colored on a light background.

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There have never been more tutorials about DIY filmmaking, so in a way you have as many options as you can think of…so long as your diffuser is the right material. You could use a bedsheet, or a thin set of curtains, or even a lampshade.

On one hand, it’s difficult to control because so many moving parts are involved — natural light, reflective material, the Kelvin scale, etc. On the other hand, how you light a shot can make all the difference in post-production and eventually the final product.

The fun thing about using a diffuser is that you can pair it with a reflective material to bounce light, which gives you control over the direction of the light, the intensity of the light, and the level of detail that the light reveals.

One answer for all of these questions is “diffused lighting.” Regardless of your lighting situation, light diffusion gives you a way to get the most out of the lighting on a set.

Then again, those options deal with video production on a set. But if you’re shooting outdoors or in a space with natural lighting, a softbox or an umbrella might not be enough for that job. Or maybe you can’t afford a new lighting kit, but you still want to create diffused light on your next project. What options do you have?

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You can get soft lighting. Think of it as getting the benefits of a directional light, but also enjoying the aesthetics of indirect lighting.

Diffusedlightarchitecture

And while you’ve probably seen (or maybe even used) a bedsheet as a light diffuser, you can also use a shower liner. The frosted plastic holds its shape better than a sheet, but you’ll want to make sure you don’t get a clear one because that plastic surface could reflect light.

The FEI (now ThermoFisher) Titan G3 50-300 PICO is a unique fourth generation transmission electron microscope which has been specifically designed for the investigation of a wide range of solid state phenomena taking place on …

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The Ernst Ruska-Centre (ER-C) for Microscopy and Spectroscopy with Electrons is a national user facility open to universities, research institutions and research laboratories in industry. The ER-C is run conjointly by Forschungszentrum Jülich GmbH and …

Project Manager for the Helmholtz Transfer Academy "Future Innovators". Interested in anything that can solve problems. Trying to unlock the potential of scientific development to also solve short-term problems of the real-world.

Polarityjam is a software suite to study endothelial cell behavior and more. Visit https://polarityjam.readthedocs.io/en/latest/index.html for more informations!

Forschungszentrum Jülich is a large-scale national research facility in the Helmholtz Association of German Research Centres. By conducting research on the global challenges currently facing society, we contribute to the welfare of mankind and responsible …

That’s not to say it’s the only obstacle, or even the most annoying. But it is ever present. We could easily lay out a half-dozen reasons why lighting is the nemesis of filmmakers.

For the purpose of this article, however, we’re going to look at some of the most popular DIY lighting solutions that help you diffuse light without spending any of your budget on new equipment. (But you may end up needing to spend a few dollars on something.)

But obviously you won’t always have access to an agreeable cloud. Your most realistic option is to find a good lighting kit, something that includes at least one type of diffuser.

DiffusedlightCeiling

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Even with nothing but the image above, you can see a pretty big difference. Diffused light is a more natural, healthy look, and — in a weird way — can actually help you balance your color temperature if you use the same light diffuser (or DIY material) on different lights.

diffused light中文

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Using a DIY technique can help you create diffused light on the cheap. More importantly, it can also help you address some of the many light-related problems that filmmakers have to solve.

You really can’t take advantage of that idea without using a light diffuser. The control it gives you doesn’t just make your footage look better — it allows you to turn any light source into something that adds depth, interest, or emotion to a shot. And that’s exactly the sort of trick a visual storyteller wants to have on any video shoot.

When you are lighting a shoot, the quality of the light can be just as influential as the color temperature or the placement. And the better the quality — whether from proximity, brightness, etc. — the more detail you’ll get out of the subject you’re trying to light.

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A pillowcase is an ingenious and inexpensive way to make a DIY softbox, catching and diffusing all of the light from a source. It works for a stand light, a lamp across the room, or — if you tape it up — even to help soften the light from a small window.

Bright-field microscopy, a method of microscopy in which the object to be observed is located in front of a bright background, called the bright field, which illuminates the microscopic image. In this process, the light passing through the object (transmitted light microscopy) or the light reflected back from it as a whole (reflected light microscopy) enters the objective of the microscope. Bright-field microscopy requires high-contrast objects, so many transmitted-light objects must be stained. In brightfield microscopy, objects appear dark or colored on a light background.Resources used"Hellfeldmikroskopie." Lexikon der Optik, Spektrum Akademischer Verlag, Heidelberg, 1999. Web. 3 Jun. 2022.

Biodiversity, functioning ecosystems, clean water and intact soils all make up our natural resource base. In the face of global change, employees at the Helmholtz Centre for Environmental Research – UFZ are united by the …

Diffusedlightphotography

The Tescan Tensor STEM is an integrated analytical precession-assisted 4D-STEM instrument for multimodal characterization of nanoscale morphological, chemical, structural and crystallographic properties of a wide range of materials systems. It is equipped with a Schottky …

Either one offers a relatively affordable option to diffuse light in your video projects. (And if you already have some lighting equipment, you could look for an individual piece — it just won’t be as good of a bargain as if you bought a bundle.)

Every filmmaker has their own ideas for DIY solutions. And chances are that as you spend more time creating content, you’ll discover a few unique techniques we might not have heard of.

Light diffusion

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As a Helmholtz centre for polar and marine research the Alfred Wegener institute works above all in the cold and temperate regions of the world. Together with numerous national and international partners we are involved …

My name is Riccardo, I am from Italy, living in Leipzig since 2012 and currently working in UFZ. I am actively working in the field of image data management and I am Data Steward in NFDI4BIOIMAGE.In the last years, …

Want to know how to deal with direct sunlight? How to add extra light sources but not cast harsh shadows? Or how to get the most out of your kit with a bounce light?

Diffusion of lightexamples

And if you want to find similar tips, tricks, and solutions to level up your filmmaking, here are some other articles from Soundstripe:

Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.