This documentation is an incomplete work in progress. There are more ways to configure and use Pulsar than are documented, and a growing number of Pulsar experts who would be more than happy to answer your questions and help with any problems you may run in to while setting up a Pulsar deployment. Please do not hesitate to reach out on the Galaxy Admins Gitter Channel

At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.

We are working to fully integrate the pE-800 into all major third party imaging software programs, but in the meantime you can use the pE-6501-8 USB controlled TTL trigger box for software control. Tutorial videos show you how to configure cellSens and MetaMorph.

Please note that as of the 0.14.0 release, Pulsar no longer supports any version of Python 2. The minimum supported Python version is 3.5.

The pE-800 is unique in offering the only 8-channel Sequence Runner available, and when combined with inline filters, transforms a manual microscope into an affordable and powerful eight-channel automated imaging system. As laboratory budgets become stretched, the pE-800 presents the ideal cost-effective Illumination System which makes high-end LED technology more accessible to life science researchers.

The -e flag to pip installs Pulsar in "editable" mode, meaning that changes you make to the source code will be reflected when running the pulsar commands installed in the virtual environment.

This project is a Python server application that allows a Galaxy server to run jobs on remote systems (including Windows) without requiring a shared mounted file systems. Unlike traditional Galaxy job runners - input files, scripts, and config files may be transferred to the remote system, the job is executed, and the results are transferred back to the Galaxy server - eliminating the need for a shared file system.

Full details on different ways to install Pulsar can be found in the install section of the documentaiton, but if your machine has the proper Python dependencies available it can be quickly download and a test job run with:

For optimum DAPI excitation, the pE-800 is available with a 365 nm or 400 nm LED for compatibility with existing filter sets. Find out more in our tutorial video here.

The pE-800 is available in the Amora Series: our proven off-the-shelf product range adapted to offer unparalleled customisation opportunities.

Adopting LED light sources for fluorescence microscopy helps laboratories become more sustainable, saving energy and reducing the carbon footprint when compared with using mercury lamps. CoolLED’s products have the following benefits:

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.

In the Lightbridge graphical user interface (see below), live samples can be further protected by balancing the irradiance to the lowest level possible while still maintaining image quality, with fine (0,1 %) control from 0-2 %, and in 1 % steps up to 100 %. And the more life-like a cell behaves, the more valuable a data set becomes.

The popular Sequence Runner program takes speed one step further and combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. Once an LED and irradiance sequence are specified in software, Sequence Runner provides lightning fast synchronised triggering via the global TTL-in of the pE-800 and a single TTL-out from a camera (or other hardware).

Software control is achieved through a fast USB 2.0 or RS-232 connection, and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:

Please note that this project is released with a Contributor Code of Conduct. By participating in this project you agree to abide by its terms.

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

This project is distributed with unit and integration tests (many of which will not run under Windows), the following command will install the needed python components to run these tests. The following command will then run these tests:

Sequence Runner Combining software control with the speed of TTL, the Sequence Runner program allows LED sequences and irradiance to be programmed via LightBridge. The sequences are controlled via global TTL from a single TTL input such as from a camera TTL out or other external hardware, and therefore requires minimal electronic hardware.

The compact pE-800 Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-low power consumption. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800 is a natural choice for cleaner, greener labs.

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Industry-leading <7 µs TTL triggering is a breakthrough for live cell imaging. Capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8  (USB controlled TTL Conversion Kit).

For manufacturers who wish to gain a competitive advantage through bright, stable and controllable illumination, CoolLED’s world-renowned LED technology is available in customised configurations.

Photons are also lost as light travels from the light source to sample plane. The only way to objectively compare light sources is by measuring irradiance at the sample plane.  To understand why we use the term irradiance and how to measure and compare light sources with accuracy and precision, download our white paper. Or please contact us if you have further questions.

The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

It is important to note that The LightBridge is only compatible with the pE-800 Series. In addition to The LightBridge, which can be downloaded here, users can also benefit from full integration into third party imaging software.

Light delivery via the standard 3 mm liquid light guide keeps the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can also be selected.

Eight individual analogue inputs allow irradiance to be controlled from 0-100 % for each channel via analogue signal (0-10 v), which is ideal for electrophysiology and optogenetics applications.

The ability to fit inline excitation filters capitalises on TTL trigger speeds, as single-band excitation filters can be installed for each channel. When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for expensive external filter wheels and overcomes the latency restrictions of mechanical movement, presenting a low-cost approach to high-speed imaging.

All eight channels can be individually controlled, with each LED selected to perfectly match the selected fluorophores. Excitation filters with white light sources let through more unwanted light, and by matching the LED this improves the signal to noise ratio for contrast-rich images revealing finer details and more information.

Musicolet

The following command will then produce a coverage report corresponding to this test and place it in the coverage_html_report subdirectory of this project.:

Tight hardware synchronisation not only increases temporal resolution, but also means samples are exposed only during acquisition, protecting against photobleaching and phototoxicity and pushing the boundaries of time-lapse studies.

Eight powerful and efficient LEDs offer broad spectral coverage from 365-740 nm for use with fluorophores ranging from DAPI to Cy7. The choice of fluorophore is no longer limited by the light source, and choosing the optimal set for each application has never been easier – even as requirements evolve over time.

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

The configuration documentation has many details on securing your Pulsar server and enabling advanced features such as cluster integration and message queue communication.

PulsarMusic Player Pro APK

Galaxy job runners are configured in Galaxy's job_conf.xml file. Some small examples of how to configure this can be found here, but be sure to check out job_conf.xml.sample_advanced in your Galaxy code base or on Github for complete information.

pE-800 Light Source: 173 mm(w) x 247 mm(d) x 174 mm(h) – Weight 3.51 kg pE-800 Power Supply: 164 mm(w) x 64 mm(d) x 35 mm(h) – Weight 0.58 kg

PulsarMusic Player APK

We are often asked about the power, intensity or irradiance of CoolLED Illumination Systems. The answer is not as simple as you might believe from many websites and data sheets, and it can be difficult to compare data from these sources as measuring set ups differ.

The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.

TTL & analogue 8 TTL inputs allow independent on/off control of each channel 8 analogue inputs 0-10 V, 0-50 kHz for dynamic control of irradiance from external analogue signals Global TTL for on/off synchronisation to camera Triggering speed <7 µs