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Dark-Field Microscopy. Examined under a dark-field microscope at ×40 or ×100 power, spirochetes appear as motile, bright corkscrews against a black background. (Reproduced with permission, from Morse SA, Holmes KK, Ballard RC, Moreland AA (eds). Atlas of Sexually Transmitted Diseases and AIDS. 4th ed. Saunders Elsevier; 2010. Copyright © 2010 Elsevier, Ltd.)

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From chancre or condyloma lata: Gently abrade the lesion with a dry gauze. Dab away any bleeding. Touch slide to exudative fluid in base of lesion. Overlay coverslip and view immediately under dark-field microscope using ×40 and ×100 objectives. From mucous patch: Touch slide to mucous patch. Overlay coverslip and view immediately under dark-field microscope using ×40 and ×100 objectives. From skin lesion: Gently scrape surface of skin lesion with edge of a number 15 scalpel blade. Dab away any bleeding. Touch slide to exudative fluid rising from skin lesion. Overlay coverslip and view immediately under dark-field microscope using ×40 and ×100 objectives.

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Compound microscope with dark-field condenser (dark-field microscope), glass microscope slide, coverslip, and physiologic saline.