[This software is NOT FOR USE with Quantifier, Assessor, Model 30/33 sound level meters – download Pulsar Analyser instead]

I would start with the largest disk, sliding it around until it is directly in the center of the light path. Increasing the illumination should then produce a good dark field effect. To optimize, first try stopping down the field diaphragm to get the best contrast between background and specimen. Try to match the size of the occulting disk to the field diameter, so that the edge of the disk is just outside the field of veiw - smaller disks are appropriate for higher power objectives. Vertically, the disk should be a close to the condenser as possible, to make the contrast the greatest. On microscopes with built-in dark field equipment, the view is so impressive because the occulting disk is built into the condenser - very close and focused. After testing the set-up this way, a stand might be rigged to fit under the microscope, so the slide can be placed in position without holding it. Something that 'grabs' the condenser and supports the occulting disks would be ideal. The less the students have to mess with, the better.

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I set this up on the crummiest little piece of garbage microscope I could find, and it looked very good. A relatively new student-model microscope should give a much better effect.

Check the PC System Requirements needed to run AnalyzerPlus.Please note downloading this version will automatically replace and update any previous version of AnalyzerPlus on your computer.

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Suspensions of cells and samples of pond water look spectacular in dark field. While specimens may look washed out and lack detail in bright field, protists, metazoans, cell suspensions, algae, and other microscopic organisms are clearly distinguished and their details show up well. At 100x you can readily see bacteria, even distinguish some structure (rods, curved rods, spirals, or cocci) and movement. Non-motile bacteria look like vibrating bright dots against a dark background. Motile bacteria can be seen moving in a definite direction, sometimes remarkably fast. In pond water samples you may find Spirillum volutans, a very large (up to 0.5 mm) motile spiral bacterium.

We’ve made some amazing changes to the software meaning that you can more easily manage and report your noise measurements in the future.Customers can now download the NEW Pulsar AnalyzerPlus software (version 2.0 and above) for use with:

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Pulsar Instruments is proud to announce the launch of our latest innovation in noise measurement technology, the Pulsar Nova Pro Environmental Noise Analyser. This state-of-the-art…

As part of Pulsar Instruments’ ongoing commitment to expanding in the Middle East, we are excited to announce partnerships with two new regional distributors. After…

Dark field optics are a low cost alternative to phase contrast optics. The contrast and resolution obtained with inexpensive dark field equipment may be superior to what you have with student grade phase contrast equipment. It is surprising that few manufacturers and vendors promote the use of dark field optics.

Customers will need to install this windows driver for the system to work. This is also accessible via the FTDI website download page.

To set up a dissecting microscope for "dark field" viewing, the specimen should be placed over an opening so that light reflects only from surfaces between cover slip and slide, not from a surface beneath the slide. You may need to make a stand to hold the slide. The surface beneath the opening should be a flat black. Turn off any built-in illuminator. Aim a high-intensity light source toward the specimen at an angle, from the top or side through a glass dish or jar.

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With a compound microscope, dark field is obtained by placing an occulting disk in the light path between source and condenser. A cheap set of occulting disks can be prepared by cutting circular pieces of black electrical tape ranging from dime-size up to a diameter that equals the width of the slide, and sticking them to the slide in a row. The circles should be spaced well apart. A specimen is placed on the microscope stage as usual, and the illumination should be made as uniform as possible. If there is an aperture diaphragm in the condenser (contrast lever), it should be opened up wide. After focusing at low power, the slide with occulting disks is placed in the light path between source and condenser, bringing it as close to the bottom of the condenser as it will go.

NOT for use with Pulsar Nova Sound Level Meters or NoisePen products – You will need Pulsar AnalyzerPlus instead (see above).

Please note this software is no longer supported by Pulsar Instruments. We recommend you use Pulsar AnalyzerPlus instead with all our noise dosemeters.

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We can’t believe that it’s already December! The team here at Pulsar Instruments would like to thank you for your continued support throughout 2023. We…

Pulsar Instruments is proud to announce the launch of our latest innovation in noise measurement technology, the Pulsar Nova Pro Environmental Noise Analyser. This state-of-the-art…

You don't need sophisticated equipment to get a dark field effect, although the effect is most dramatic when the occulting disk is built into the condenser itself. You do need a higher intensity light, since you are seeing only reflected light. At low magnification (up to 100x) any decent optical instrument can be set up so that light is reflected toward the viewer rather than passing through the object directly toward the viewer.

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This is the previous version of the new AnalyzerPlus software. We recommend you use the new version as this version is no longer supported. If for some reason you still need to access the old version you can download it here:

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To view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scattered by objects on the slide can reach the eye (figure 2). Instead of coming up through the specimen, the light is reflected by particles on the slide. Everything is visible regardless of color, usually bright white against a dark background. Pigmented objects are often seen in "false colors," that is, the reflected light is of a color different than the color of the object. Better resolution can be obtained using dark field as opposed to bright field viewing.

We can’t believe that it’s already December! The team here at Pulsar Instruments would like to thank you for your continued support throughout 2023. We…

Windows system requirements: Customers will need to install this driver for the system to work. This is also accessible via the FTDI website download page.

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As part of Pulsar Instruments’ ongoing commitment to expanding in the Middle East, we are excited to announce partnerships with two new regional distributors. After…

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A dilute suspension of yeast cells makes a good practice specimen for dark field optics, particularly when cultured with living Paramecium.

Dark field illumination is most readily set up at low magnifications (up to 100x), although it can be used with any dry objective lens. Any time you wish to view everything in a liquid sample, debris and all, dark field is best. Even tiny dust particles are obvious. Dark field is especially useful for finding cells in suspension. Dark field makes it easy to obtain the correct focal plane at low magnification for small, low contrast specimens. Use dark field for