First of all, would a brightfield microscope/darkfield microscope be the best for your needs? There are high-end microscopes that are designed to work equally well in brightfield and darkfield. These add versatility to your line-up and can offer cost savings.

dark-field microscopy

Following the acquisition of the Georgian startup in 2021, Impel's digital engagement platform combines automotive industry-leading Conversational AI technology with best-in-class applications for digital sales, automated communication, and imaging. The company's continuous technology engages customers in the purchasing process, informs them of news, and influences their decisions. Impel's technological solutions are designed to enable car dealers to create services for customers that provide the fastest and most convenient relationship between companies and customers.

The Stratus Award is given to companies that excel in cloud-based innovation. Other winners of this year's Stratus Award, along with Impel, include companies such as Hewlett Packard, IBM, Red Hat.

One thing that’s great about darkfield microscopy is that you can convert a brightfield microscope so you can take advantage of darkfield illumination. One way to do this, if your microscope is able, is to switch the current condenser with a darkfield condenser.

"Impel is a leading company in driving practical innovation in the cloud," said Maria Jimenez, Business Intelligence Group's Chief Nominations Officer.

Brightfield microscopes use a light to illuminate a specimen. The light is gathered by the microscope’s condenser, transmitted through the specimen, and then passes through the microscope’s objective lens. The contrast in the sample is generated by the absorption of light. The result is a bright background.

A darkfield microscope uses a darkfield condenser to produce the background or the field of a specimen to appear dark while using a specialized illumination technique to make the subject itself appear bright.

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"Winning the Stratus Award in the category of human intelligence with companies of such a level, which we can consider as one of the most high-tech fields of our time, proves that Georgia is strengthening its position on the map of world innovation even more. We are glad that the technology created by the Georgian team is a completely new word. It's amazing to be at the forefront of such a high-tech field and industry. For Impel, receiving this award motivates us, even more, to continue moving towards our ambitious plans with the joint involvement of our Georgian and American teams," says Dachi Choladze, Pulsar AI co-founder and head of innovation at Impel.

Would a darkfield kit paired with an existing brightfield microscope work? Some laboratories find that kits that are specialized to convert brightfield microscopes into darkfield microscopes are all that they need to get double-duty function from their existing equipment. This is achieved with a darkfield condenser or darkfield slider that goes in a brightfield condenser.

In the darkfield microscope, as mentioned above, the light goes through the specimen before heading to the objective lens. This leads to impressive magnification. In a phase contrast microscope, part of the light beam is modified by the specimen, and part of it Is not. This is the contrast, between light that has gone through the specimen and light that hasn’t. When the lights have this contrast, you get the interference of light rays that produce a high contrast allowing for illumination of transparent biological samples.

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Darkfield microscopes feature a specialized condensing system, which includes a darkfield condenser or a darkfield slider that fits into the brightfield condenser. This component effectively blocks direct condenser rays from entering the microscope's objective lens. Instead, the light is scattered by the subject through reflection, refraction, or diffraction and then transmitted to the objective lens. This gives the subject a brightness and the background appears dark.

It should be noted that darkfield microscopy has some limitations, including the fact that exposure to intense lighting can be hazardous to some specimens. Darkfield can also be less beneficial when working with thick specimens, and it can make it more difficult to identify internal details.

In general, darkfield microscopy is a good option for suspension samples, liquids, and specimens with a low contrast. It’s also especially useful when viewing live organisms in water samples.

Impel (formerly SpinCar), which acquired the Georgian artificial intelligence startup Pulsar AI, was recognized as a global leader in artificial intelligence.

If your needs are such that having a dedicated darkfield microscope is beneficial, there are a few things you should know when buying a darkfield microscope.

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Bright field dark field

Beyond that, you will want to apply general microscope buying guidelines to your purchase decision. Consider how you will be using the microscope, who will be using it, and what magnification and resolution are needed. And one of the most important considerations is who you purchase your microscope from. A reputable dealer is essential because you’ll want to be sure you’re getting what you paid for, that there’s a warranty to back up your purchase, and that the dealer is knowledgeable so you can return with questions when they arise.

There are many other situations where darkfield microscopy is used, it can be found in the fields of biology, gemology, metallurgical sciences, and industry. These microscopes are great for getting a good look at the following:

The technology created by the Georgian company Pulsar AI won the Stratus Award in the artificial intelligence category by the Business Intelligence Group. The award was given to Impel (formerly SpinCar), a global leader in automotive digital engagement technology, which bought the Georgian startup "Pulsar AI" last year.

Each type of microscope has subjects that it’s better at viewing, which can customize the uses quite a bit. A few of the more popular darkfield microscope uses include situations where the specimens are live and using a stain for contrast in a brightfield situation would kill the sample.