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The revolution series Orange traffic cone will change the way you think about Orange traffic cones. Vehicle and pedestrian traffic safety during rush hours and at night can be difficult. In such circumstances, not only are the chances of accidents more probable but drivers and pedestrians tend to ignore traffic control tools and equipment. This problem is exacerbated if the Orange traffic cones are broken, faded, or have tipped over due to other factors such as vehicle impacts, storms, or strong winds. To ensure the safety of foot and vehicle traffic, you need to use Injection molded DOT Orange traffic cones with a black base. The revolution series Orange traffic cone is a line of innovative, high-quality, cost-effective Orange Traffic cones featuring our patented circle interlocking system and leveling differential mechanism, delivering outstanding performance under the harshest conditions. There’s much more to the design than meets the eye. The patented leveling differential mechanism involves strategically placed cavities inside the base to provide a stronger foundation, allowing the JBC® Orange traffic cones to absorb repeated impacts resulting in a virtually indestructible base. The revolution series traffic cones are made using high-quality premium grade PVC injection molding technology, resulting in the most durable Orange traffic cones available in the North American market. The Patented process gives you the best of both worlds – high quality at a low price. Orange traffic cones are 100% fluorescent on both the inner and outer layers, the industry’s first and only.

36″ Orange traffic cones are the largest Orange cone available and is approved for use on high-speed roadways > 45 Mph. The fact that this cone weighs upwards of 10 lbs provides adequate resistance to tipping over in heavy winds or backdraft from trucks and cars.

Löschberger, A. et al. Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. J. Cell. Sci. 125, 570–575 (2012).

Visibility is the key to avoiding accidents. Injection-molded Traffic pylons are used to warn motorists of potential hazards to prevent mishaps and accidents and improve the flow of traffic in sensitive areas such as construction sites, repair work at roadsides, public gatherings in dense traffic areas.

Katayama, H., Yamamoto, A., Mizushima, N., Yoshimori, T. & Miyawaki, A. GFP-like proteins stably accumulate in lysosomes. Cell. Struct. Funct. 33, 1–12 (2008).

Uno, Sn., Kamiya, M., Yoshihara, T. et al. A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging. Nature Chem 6, 681–689 (2014). https://doi.org/10.1038/nchem.2002

Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91, 4258–4272 (2006).

We have a 30-day return policy, which means you have 30 days after receiving your item to request a return. To be eligible for a return, your item must be in the same condition that you received it, and in its original packaging. You’ll also need the receipt or proof of purchase. To start a return, you can fill out the ORDER RETURN FORM HERE. If your return is accepted, we’ll send you the instructions on how and where to send your package. Items sent back to us without first requesting a return will not be accepted.

Center for International Research on Micronano Mechatronics, Institute of Industrial Science, The University of Tokyo, Tokyo, 153-8505, Japan

Silkscreens: A silkscreen will allow you the most flexibility in fonts, styles, logos and designs to appear on your cones. Silkscreen set up fees is a one time charge of $130.00

Kamiya, M. et al. β-Galactosidase fluorescence probe with improved cellular accumulation based on a spirocyclized rhodol scaffold. J. Am. Chem. Soc. 133, 12960–12963 (2011).

Exceptions / non-returnable items Certain types of items cannot be returned, like custom products (such as special orders or personalized items). Please get in touch if you have questions or concerns about your specific item. Certain Items will Carry restocking fees for us to take back into our stock, This is due to items being made to order or for being an oversized item. Exchanges

Los, G. V. et al. Halotag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem. Biol. 3, 373–382 (2008).

Fölling, J. et al. Photochromic rhodamines provide nanoscopy with optical sectioning. Angew. Chem.Int. Ed. 46, 6266–6270 (2007).

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Corrie, J. E. T. et al. Ring-chain interconversion of sulforhodamine-amine conjugates involves an unusually labile C–N bond and allows measurement of sulfonamide ionization kinetics. J. Phys. Org. Chem. 21, 286–298 (2008).

Kenmoku, S., Urano, Y., Kojima, H. & Nagano, T. Development of a highly specific rhodamine-based fluorescence probe for hypochlorous acid and its application to real-time imaging of phagocytosis. J. Am. Chem. Soc. 129, 7313–7318 (2007).

The 3M 3840 Hi-intensity reflective cone collars complement the high quality of the revolution series DOT Orange traffic cones. Having such a high quality reflective cone collar demonstrates the level of quality these Orange traffic cones provide during day or night. 3M high intensity is a plasticizer resistant encapsulated reflective lens sheeting with pressure-sensitive adhesive. The 3M 3840 cone sheeting is specifically designed to reflectorize the JBC® Orange traffic cones, thus meeting the MUTCD requirements for highway and utility use. Accidents can happen at any time, day, or night. When they do reflective Orange traffic cones are the first choice in temporary traffic systems for ensuring the safety of work crews, first responders, and other motorists. They are easy to install and pick up after the work has been completed. Moreover, these DOT Orange traffic cones are especially useful in densely populated metropolitan areas where daytime work is too dangerous or too invasive to traffic.3M 3840 reflective cone collars have a full contact pressure-sensitive adhesive that stands up to repeated abuse even after multiple impacts at 50 mph.

Maeshima, K. et al. Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase. Nature Struct. Mol. Biol. 17, 1065–1071 (2010).

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Do you often struggle with setting up your traffic management system? Are you worried about damaging the products while transporting them? At Cabletiesandmore.com we offer quality, value, convenience, and economy to all our customers. We offer carts with increased capacity. Each product sent to you is checked by our monitoring team to ensure that you always receive the highest quality products from us!

The revolution series traffic cones with a black base have a patented leveling system that holds traffic cones in a vertical position and ensures the cones will not easily tip over.

Lukinavičius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chem. 5, 132–139 (2013).

Funakoshi, T. et al. Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes. FEBS Lett. 581, 4910–4916 (2007).

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Revolution series Injection-molded traffic cones are manufactured with 3M 3840 Hi-intensity reflective collars to maximize visibility at any time of the day. 3M high-intensity collar's reflectivity is achieved with the use of plasticizer resistant encapsulated lens reflecting sheeting for achieving pressure-sensitive adhesiveness. 3M 3840 cone sheeting is a specialized technique for improving the reflection of cones and meeting the standards of MUTCD.

The fastest way to ensure you get what you want is to return the item you have, and once the return is accepted, make a separate purchase for the new item.

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When deciding which Orange traffic cone you need for your application, you first should be sure of road laws regarding the Orange traffic cone height required for the roadway speed during use. Moreover, you will need to buy cones with reflective sheeting if your application will involve using the Orange cones at nighttime. Reflective sheeting to improve visibility, even during peak traffic hours or at night.

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12″ Orange traffic Cones are not approved for legal use on public roads. 12″ JBC traffic Cones are primarily designed for use in private area direction of vehicles, athletics, and physical training environments.

Stencils: A stencil is a simple stencil font ( lettering) that does not allow for logos or complex artwork. Stencil setup fee is a one time charge of $45.00

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28″ Orange Traffic Cones are designed for use on Roadways, where the Speed is Greater than 45 mph. 28″ Traffic cones are the most popularly used traffic cone in highway maintenance, roadwork projects, and construction.

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Folling, J. et al. Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nature Methods 5, 943–945 (2008).

Rust, M. J., Bates, M. & Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nature Methods 3, 793–796 (2006).

Quan, T., Zeng, S. & Huang, Z-L. Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal–oxide semiconductor, and charge-coupled devices for superresolution imaging. J. Biomed. Opt. 15, 066005 (2010).

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Damages and issues Please inspect your order upon reception and contact us immediately if the item is defective, damaged or if you receive the wrong item, so that we can evaluate the issue and make it right.

The fastest way to ensure you get what you want is to return the item you have, and once the return is accepted, make a separate purchase for the new item.

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S.U., M.K., T.Y., Y.O., S.T. and Y.U. conducted experiments and performed analyses. S.U., M.K., Y.O. and Y.U. co-wrote the manuscript. K.S., K.O., M.C.T., T.F. and H.F. contributed materials/analysis tools. M.K. and Y.U. planned and initiated the project, designed experiments, and supervised the entire project.

Hinner, M. J. & Johnsson, K. How to obtain labeled proteins and what to do with them. Curr. Opin. Biotechnol. 21, 766–776 (2010).

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Sakabe, M. et al. Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization. J. Am. Chem. Soc. 135, 409–414 (2013).

Koide, Y., Urano, Y., Hanaoka, K., Terai, T. & Nagano, T. Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. J. Am. Chem. Soc. 133, 5680–5682 (2011).

Looking for a quotation ? We would be happy to help by providing a quick response, inventory status, and Volume Level pricing, when available. Please fill out the form below and a representative will be in touch with you within hours to one business day to answer any questions and quote your request.

Kim, H. N., Lee, M. H., Kim, H. J., Kim, J. S. & Yoon, J. A new trend in rhodamine-based chemosensors: application of spirolactam ring-opening to sensing ions. Chem. Soc. Rev. 37, 1465–1472 (2008).

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Fu, M., Xiao, Y., Qian, X., Zhao, D. & Xu, Y. A design concept of long-wavelength fluorescent analogs of rhodamine dyes: replacement of oxygen with silicon atom. Chem. Commun. 1780–1782 (2008).

Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M. & Zhuang, X. Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Nature Methods 8, 1027–1036 (2011).

Szymborska, A. et al. Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging. Science 341, 655–658 (2013).

The revolution series traffic cones with a black base have a patented leveling system that holds traffic cones in a vertical position and ensures the cones will not easily tip over.

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Heilemann, M. et al. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew. Chem. Int. Ed. 47, 6172–6176 (2008).

Huang, B., Babcock, H. & Zhuang, X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell 143, 1047–1058 (2010).

Stencils: A stencil is a simple stencil font ( lettering) that does not allow for logos or complex artwork. Stencil setup fee is a one time charge of $45.00

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Tokunaga, M., Imamoto, N. & Sakata-Sogawa, K. Highly inclined thin illumination enables clear single-molecule imaging in cells. Nature Methods 5, 159–161 (2008).

Are we missing the specification you need? Please go to our Contact Us page and submit your question to us, or call 1-877-284-7760

van de Linde, S., Heilemann, M. & Sauer, M. Live-cell super-resolution imaging with synthetic fluorophores. Annu. Rev. Phys. Chem. 63, 519–540 (2012).

Steinhauer, C., Forthmann, C., Vogelsang, J. & Tinnefeld, P. Superresolution microscopy on the basis of engineered dark states. J. Am. Chem. Soc. 130, 16840–16841 (2008).

Silkscreens: A silkscreen will allow you the most flexibility in fonts, styles, logos and designs to appear on your cones. Silkscreen set up fees is a one time charge of $130.00

This research was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid for Scientific Research (KAKENHI): 20117003 and 23249004 to Y.U., 23113504 and 25113707 to M.K., 23710108 to M.C.T., 24659092, 25113723 and 25293046 to Y.O. and 21121004 to T. F.), by The Daiichi-Sankyo Foundation of Life Science (to Y.U.), by the Uehara memorial Foundation (to Y.O.), by The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to M.K.) and by a JSPS stipend (to S.U.). The authors thank D. Toomre for many useful discussions, K. Johnsson and G. Lukinavičius for β-tubulin–SNAP plasmid, N. Imamoto for HeLa cell lines expressing nuclear pore proteins, R. Wong for POM121 plasmid, J. Asada and S. Dan Xu for the construction of plasmids and experimental assistance, and Y. Arai for the ImageJ script for analysing projection profiles of the localization of super-resolution images. The African green monkey kidney normal cell line Vero (JCRB0111) was obtained from the Japanese Collection of Research Bioresources (JCRB) cell bank.

Refunds We will notify you once we’ve received and inspected your return, and let you know if the refund was approved or not. If approved, you’ll be automatically refunded on your original payment method. Please remember it can take some time for your bank or credit card company to process and post the refund too.

Koide, Y., Urano, Y., Hanaoka, K., Terai, T. & Nagano, T. Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer. ACS Chem. Biol. 6, 600–608 (2011).

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van de Linde, S. et al. Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nature Protoc. 6, 991–1009 (2011).

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Belov, V. N., Bossi, M. L., Fölling, J., Boyarskiy, V. P. & Hell, S. W. Rhodamine spiroamides for multicolor single-molecule switching fluorescent nanoscopy. Chem. Eur. J. 15, 10762–10776 (2009).

Single-molecule localization microscopy is used to construct super-resolution images, but generally requires prior intense laser irradiation and in some cases additives, such as thiols, to induce on–off switching of fluorophores. These requirements limit the potential applications of this methodology. Here, we report a first-in-class spontaneously blinking fluorophore based on an intramolecular spirocyclization reaction. Optimization of the intramolecular nucleophile and rhodamine-based fluorophore (electrophile) provide a suitable lifetime for the fluorescent open form, and equilibrium between the open form and the non-fluorescent closed form. We show that this spontaneously blinking fluorophore is suitable for single-molecule localization microscopy imaging deep inside cells and for tracking the motion of structures in living cells. We further demonstrate the advantages of this fluorophore over existing methodologies by applying it to nuclear pore structures located far above the coverslip with a spinning-disk confocal microscope and for repetitive time-lapse super-resolution imaging of microtubules in live cells for up to 1 h.

Jones, S. A., Shim, S.-H., He, J. & Zhuang, X. Fast, three-dimensional super-resolution imaging of live cells. Nature Methods 8, 499–505 (2011).

Are we missing the specification you need? Please go to our Contact Us page and submit your question to us, or call 1-877-284-7760