The audio and video recording device will document police and civilian interactions while engaged in enforcement and investigative duties.

Szymborska, A. et al. Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging. Science 341, 655–658 (2013).

Sakabe, M. et al. Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization. J. Am. Chem. Soc. 135, 409–414 (2013).

S.U., M.K., T.Y., Y.O., S.T. and Y.U. conducted experiments and performed analyses. S.U., M.K., Y.O. and Y.U. co-wrote the manuscript. K.S., K.O., M.C.T., T.F. and H.F. contributed materials/analysis tools. M.K. and Y.U. planned and initiated the project, designed experiments, and supervised the entire project.

Heilemann, M. et al. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew. Chem. Int. Ed. 47, 6172–6176 (2008).

Uno, Sn., Kamiya, M., Yoshihara, T. et al. A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging. Nature Chem 6, 681–689 (2014). https://doi.org/10.1038/nchem.2002

Fölling, J. et al. Photochromic rhodamines provide nanoscopy with optical sectioning. Angew. Chem.Int. Ed. 46, 6266–6270 (2007).

Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91, 4258–4272 (2006).

Spontaneouslyperson

Löschberger, A. et al. Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. J. Cell. Sci. 125, 570–575 (2012).

HYCON HPP18E is equipped with an 11-kW motor and is available as a 30 lpm and a 40 lpm version. The perfect power pack for professional and specialized companies within concrete drilling, cutting, and others.

Fu, M., Xiao, Y., Qian, X., Zhao, D. & Xu, Y. A design concept of long-wavelength fluorescent analogs of rhodamine dyes: replacement of oxygen with silicon atom. Chem. Commun. 1780–1782 (2008).

Rust, M. J., Bates, M. & Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nature Methods 3, 793–796 (2006).

Belov, V. N., Bossi, M. L., Fölling, J., Boyarskiy, V. P. & Hell, S. W. Rhodamine spiroamides for multicolor single-molecule switching fluorescent nanoscopy. Chem. Eur. J. 15, 10762–10776 (2009).

Spontaneouslymeaning

Construction Site Entry Signs are signs displayed at the entrance to building and construction sites and provide important information about the site including ...

May 9, 2024 — WilmerHale advised Dedrone, a global leader in airspace security, in its definitive acquisition agreement to Axon, the global public safety ...

Caution - Floor slippery when wet safety sign is available in ABS and Chromadek.

Spontaneouslycombust

A computer-aided dispatch system for law enforcement serves as a permanent record of each activity during a given shift. When incorporated with an agency's ...

Hinner, M. J. & Johnsson, K. How to obtain labeled proteins and what to do with them. Curr. Opin. Biotechnol. 21, 766–776 (2010).

Quan, T., Zeng, S. & Huang, Z-L. Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal–oxide semiconductor, and charge-coupled devices for superresolution imaging. J. Biomed. Opt. 15, 066005 (2010).

How to pronouncespontaneously

Kamiya, M. et al. β-Galactosidase fluorescence probe with improved cellular accumulation based on a spirocyclized rhodol scaffold. J. Am. Chem. Soc. 133, 12960–12963 (2011).

Tokunaga, M., Imamoto, N. & Sakata-Sogawa, K. Highly inclined thin illumination enables clear single-molecule imaging in cells. Nature Methods 5, 159–161 (2008).

This research was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant-in-Aid for Scientific Research (KAKENHI): 20117003 and 23249004 to Y.U., 23113504 and 25113707 to M.K., 23710108 to M.C.T., 24659092, 25113723 and 25293046 to Y.O. and 21121004 to T. F.), by The Daiichi-Sankyo Foundation of Life Science (to Y.U.), by the Uehara memorial Foundation (to Y.O.), by The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to M.K.) and by a JSPS stipend (to S.U.). The authors thank D. Toomre for many useful discussions, K. Johnsson and G. Lukinavičius for β-tubulin–SNAP plasmid, N. Imamoto for HeLa cell lines expressing nuclear pore proteins, R. Wong for POM121 plasmid, J. Asada and S. Dan Xu for the construction of plasmids and experimental assistance, and Y. Arai for the ImageJ script for analysing projection profiles of the localization of super-resolution images. The African green monkey kidney normal cell line Vero (JCRB0111) was obtained from the Japanese Collection of Research Bioresources (JCRB) cell bank.

Huang, B., Babcock, H. & Zhuang, X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell 143, 1047–1058 (2010).

Our cameras. At the Calgary Police Service, we have two types of cameras that our frontline officers use: Body ...

van de Linde, S. et al. Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nature Protoc. 6, 991–1009 (2011).

Jones, S. A., Shim, S.-H., He, J. & Zhuang, X. Fast, three-dimensional super-resolution imaging of live cells. Nature Methods 8, 499–505 (2011).

van de Linde, S., Heilemann, M. & Sauer, M. Live-cell super-resolution imaging with synthetic fluorophores. Annu. Rev. Phys. Chem. 63, 519–540 (2012).

Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M. & Zhuang, X. Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Nature Methods 8, 1027–1036 (2011).

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Spontaneouslyin a sentence

Automated fraud detection systems work by using advanced algorithms and machine learning techniques to analyse large volumes of data and detect patterns and ...

Maeshima, K. et al. Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase. Nature Struct. Mol. Biol. 17, 1065–1071 (2010).

Spontaneouslysynonym

Koide, Y., Urano, Y., Hanaoka, K., Terai, T. & Nagano, T. Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. J. Am. Chem. Soc. 133, 5680–5682 (2011).

Katayama, H., Yamamoto, A., Mizushima, N., Yoshimori, T. & Miyawaki, A. GFP-like proteins stably accumulate in lysosomes. Cell. Struct. Funct. 33, 1–12 (2008).

Folling, J. et al. Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nature Methods 5, 943–945 (2008).

Koide, Y., Urano, Y., Hanaoka, K., Terai, T. & Nagano, T. Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer. ACS Chem. Biol. 6, 600–608 (2011).

Corrie, J. E. T. et al. Ring-chain interconversion of sulforhodamine-amine conjugates involves an unusually labile C–N bond and allows measurement of sulfonamide ionization kinetics. J. Phys. Org. Chem. 21, 286–298 (2008).

Funakoshi, T. et al. Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes. FEBS Lett. 581, 4910–4916 (2007).

Batteries, Chargers & Accessories · Flashlight Pouches. HYDRATION. Hydration Packs · Bottles & Drinkware · Hydration Accessories. BATONS & HANDCUFFS. Batons ...

Lukinavičius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chem. 5, 132–139 (2013).

Center for International Research on Micronano Mechatronics, Institute of Industrial Science, The University of Tokyo, Tokyo, 153-8505, Japan

Kim, H. N., Lee, M. H., Kim, H. J., Kim, J. S. & Yoon, J. A new trend in rhodamine-based chemosensors: application of spirolactam ring-opening to sensing ions. Chem. Soc. Rev. 37, 1465–1472 (2008).

Spontaneouslymeaning in Urdu

Spontaneouslymeaning in Hindi

Los, G. V. et al. Halotag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem. Biol. 3, 373–382 (2008).

AV:Link 100.550 PBH10 Blk Bluetooth Headphones Wireless Music SD Card Noise Cancelling. £19.99. In Stock. 0 Votes. Add To Basket.

Single-molecule localization microscopy is used to construct super-resolution images, but generally requires prior intense laser irradiation and in some cases additives, such as thiols, to induce on–off switching of fluorophores. These requirements limit the potential applications of this methodology. Here, we report a first-in-class spontaneously blinking fluorophore based on an intramolecular spirocyclization reaction. Optimization of the intramolecular nucleophile and rhodamine-based fluorophore (electrophile) provide a suitable lifetime for the fluorescent open form, and equilibrium between the open form and the non-fluorescent closed form. We show that this spontaneously blinking fluorophore is suitable for single-molecule localization microscopy imaging deep inside cells and for tracking the motion of structures in living cells. We further demonstrate the advantages of this fluorophore over existing methodologies by applying it to nuclear pore structures located far above the coverslip with a spinning-disk confocal microscope and for repetitive time-lapse super-resolution imaging of microtubules in live cells for up to 1 h.

Steinhauer, C., Forthmann, C., Vogelsang, J. & Tinnefeld, P. Superresolution microscopy on the basis of engineered dark states. J. Am. Chem. Soc. 130, 16840–16841 (2008).

Rapid Response Firefighting Helicopter. First up on our firefighter tools list is a firefighting helicopter. · Personal Protective Equipment · Fire Hose · Fire ...

Kenmoku, S., Urano, Y., Kojima, H. & Nagano, T. Development of a highly specific rhodamine-based fluorescence probe for hypochlorous acid and its application to real-time imaging of phagocytosis. J. Am. Chem. Soc. 129, 7313–7318 (2007).