And don’t forget to take some test shots! Adjusting the lighting, the camera, and the subject to achieve the desired effect might take some trial and error. In addition to this, posing your subject against a dark-colored background can help you draw more attention to the subject.

I use since more than two years the Italian Optika B-500TDK darkfield microscope in my doctor's office and I am with workmanship and image quality very satisfied. It is hard to find a difference in quality compared with similar products which are 3 to 4 times more expensive and which are usually propagated in the courses by the speakers. I am also very impressed by the LED light. With only 4 Watts of power consumption, it shows no heating effect of the sample, and so no noisy cooler disturbs as with the usual 100-Watts halogen lamps." Dr. Helge Richter, General Practitioner, 1010 Vienna, Austria My company organizes dark field seminars for all levels with professors from all around the world. In our training center in Vienna on the one hand, the theory basics are teached, on the other hand, the participants should get acquainted with the practical work with the dark field microscope. We use in our trainings the OPTIKA the B-500TDK: excellent image, silent and easy to use. Many of our participants made already the same choice as we did. Maria Mudro, CEO of „Gesundheitsakademie Wien“, Otto- Bauer- Gasse 20, 1060 Vienna, Austria I bought end of 2014 my dark field microscope OPTIKA B383-DK from Sarras eU. When I unpacked my new microscope for the first time, I was impressed with how stable and "scientific" it looked like. The assembly of the microscope was quick and effortless. As a medical scientist (Onkogenetics - pathologist) I have always worked with top microscopes of the most famous manufacturers. I can confirm that working with this dark field microscope is more than sufficient, you don’t need any more to look further for more expensive or larger microscopes. In my opinion, an advantage of the B383-DK microscope is that this model is both compact and lighter than the B500-TDK, on the other hand, just as "professional" to work in the dark field. It enables easy transportation for home visits as it fits easily into a microscope suitcase. Ideal for the office, but also to take it with you! Dr. Edwin A. King M.Sc. Ph.D., Biomedical - DFM consultant and therapist, Vienna, Austria

Fluorescence microscope

Silhouettes are often an intriguing photo element and can be creatively used to evoke interest in a scene and bring attention to a subject’s outline. In order to create a perfect silhouette, completely obstruct the view of the camera and the main light source by bringing the subject directly in front of the light source. This leads to a blacked-out subject with no to very limited light toward them.

Even though you can opt to shoot backlit images at any time of the day, a window of some specific hours favors the process naturally. That time of the day when the sun naturally acts as a soft natural light source and offers a beautiful glow can really bring the best out of your backlit images. Some of the best times to capture this radiant orange glow is either in the morning after the sun rises or right before it sets as the day comes to an end (often known as the golden hour).

While you might be tempted to use this technique to add a glint of light to your images, backlighting might not be the ideal lighting setup for every scenario. In fact, at times, it might not even be right for your scene at all. That is because not all subjects could be easily backlit. However, the correct understanding of how and when to use this lighting technique can help you give your images the look you desire. So if you have been struggling to understand why and how to use backlight for creative purposes, this list might give you some clarity.

To begin with, to understand what is backlight or backlighting in photography, let’s take a look at a standard photography lighting scenario. In any lighting scenario, we have three main components - the light source, the subject, and the camera. And any or all variations in a lighting scene are a result of either change in the position of these components or the quality of light. Because apart from determining how dark or bright your image is, the way you light a scene plays a big role in setting the look and feel of your image - especially the mood and tone you choose to depict through the photos that you create. The principle is simple - how you manipulate light in the images that you take will be a key deciding factor in the creative approach you take in telling your visual story. Now, let’s get to the technique!

On-site setup Assembly of the microscope, software setup, calibration and technical enrolment. Customers who have already worked with a dark field microscope will normally not need this services. If you have never worked with a dark field microscope before, however we recommend you to book our service. The cost for this service depends on your location. Support during warranty cases: Should it ever come to a problem, you just need to notify us - we organize for you with the technical support team of the manufacturer the steps needed. Please note that the warranty period for each unit, guarantee conditions and repair times depend on the guarantee conditions of the respective manufacturer.

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One of the best ways to bounce the light back to the subject’s face or front is through the use of a reflector. Especially, when you are photographing in a natural light setting, the light is ever-changing, and therefore properly lighting up your subject can be a bit of a challenge. However, with the use of reflectors, you can fill in the shadows that form on your subject’s face/front by using soft light.

To select all lights illuminating a specific surface, select the surface, then from the Rendering menu set, select Lighting/shading > Select Lights ...

Dark fieldscattering spectroscopy

Since 06.05.2010 when we sold our first darkfield microscope (at that time an OPTIKA B353DK) we received from our customers a number of questions about dark field technology. On this page you can find answers to the most frequently asked questions concerning the selection of the technical dark field equipment. You will also find information about our offer, sample captures taken by our offered equipment, video tutorials and some customer opinions.

At times, this kind of backlighting can help tell a story and bring a change in perspective. However, to make the silhouettes appear interesting, it is essential to keep two things in mind -

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No - depending on the model, a camera with less megapixels and a high-quality sensor, can have a better picture than one with more megapixels but lower quality sensor. The image quality of the camera (e.g. making visible of very fine structures) depends on various technical parameters such as of the dynamic range of the sensor. You should watch the image quality live or see video recordings to judge the quality by yourself - the quality differences are large and just the number of pixels is not enough for the decision.

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Sun flare is a common occurrence in the process of creating images with backlight. However, it helps to learn how to create it effectively as a creative element to add to your scene. One of the ways to get sun flare (or a sunlight peek-a-boo effect) is to place the camera in the position of backlighting and then simply move the camera slowly until you come to a spot that gives you the desired amount of sun flare. In addition to your subject, also use the surroundings of the subject as an object to create the sun flare effect. Trees often make for a great object for peeking sun flare backlighting effect.

LED technology has revolutionized many areas of technology - including microscopy. Most manufacturers equip their microscopes now from halogen to LED. We recognize this trend very significantly not only in the dark field but in all types of microscopes, which we distribute. The LED lighting has many advantages - some of which are as follows. Lifetime - The longevity of the LED (50,000 hours according to manufacturer OPTIKA) eliminates the permanent replacement of lighting halogen lamps. Color of the light source - The use of white LEDs eliminates the "Yellow tinge", which is visible in the halogen lamps. Silent and compact - So far, the halogen lamps were usually in an external device and the light has been brought with light guides to the condenser. The halogen lamp had to be cooled with a fan, which is loud. Using LED lighting you do not need a second external device and operate compact and noiseless. Low power consumption - While consumption of halogen lamps is about 100-150 Watts, LED consumes depending on the model up to 7 Watts.

Rim light is a type of lighting setup where the subject is positioned to completely block the main light source, therefore creating a highlighted outline around the subject. This type of backlighting provides a dramatic halo-like look and is often used in product photography and moody and dramatic photography. Moreover, it helps to create a sense of separation between the subject and the background all the while highlighting the subject’s contours and shape.

Another integral factor that you need to get right to bring out the best of colors in the scene is white balance. White balance allows you to adjust the intensities of colors in the scene for the camera to process them as close to the natural colors as possible. However, since shooting with a backlight involves adjusting the camera settings as per multiple lighting setups, it could get a bit tricky to get it right. To learn more about what is white balance, and how to adjust it to achieve more natural colors, give this article a read. Even though you could try to fix your photo’s white balance during the post-processing phase, it is just less time-consuming and simpler to get it right at the very beginning.

Quick Tip: If you are shooting on a day when the sun is not visible (or even after the sun has set), you can still create a backlit image for your clients with a little help from a flash. Just place the light behind the subjects, and you’ll see how it highlights them with a gorgeous rim light.

Please note here that our HDMI cameras can also be used as USB cameras, since next to the HDMI they have also have a USB output - the picture quality is, however, in HDMI mode higher. In general, the trend of our customers goes toward HDMI cameras. Setup - HDMI cameras have the advantage that they connect directly to any monitor or TV device having a HDMI input - so you do not need a PC and no software setup. Connect the cables, turn on te camera and the picture is here! Frames per Second - The signals from the HDMI cameras are not "slowed down" through the USB channel of the PC and therefore reach very high frame rates. Our HDMI cameras achieve in Full HD resolution 60 fps while USB cameras achieve depending on the model between 7-25 fps. Camera Control - With USB cameras you can control all parameters (brightness, contrast, etc.) through the software on the PC. HDMI cameras have an on-screen menu that is displayed on the screen - there you can control all the parameters. Image/Video capture - USB cameras capture photos on the PC using their application. With HDMI cameras the photos are recorded on a removable SD card, which is located on the camera. If necessary, they can always be transferred to a PC.

Throw in a stunning pose to this composition, and it might fetch you a thumbs up from your clients as well as their friends and family when they share the pictures on social media. And when it comes to social media, we don’t even need to tell you that the more the likes, the better!

Yes - although this is more the exception, we have customers who do not need a live view on the screen and prefer to work with their own camera. For the connection you need a so-called T/2 connecting ring from the manufacturer of your SLR (available at photo shops) and the "matching" adapter from microscope manufacturers, which we deliver. The "matching" adapter because depending on the modelof your SLR, usually you have a full-frame or APS-C sensor inside. These sensor types have different sizes and therefore need a different adapter to cut down the microscope image to the "right" size. When choosing a wrong adapter you will see either only a small part of the image or will have black areas at the outer borders. We can assist you with the configuration of such setups.

When backlighting your subjects, the sun does not always need to be in the full frame of your camera. Knowing exactly how to master the sun flare look while still keeping your subjects properly exposed is not the easiest look to achieve; so start by diffusing the sun. Find a location where the sun can be partially covered by your camera, while still allowing the light to shine onto your subjects. How? Just look for spots that have buildings or trees. These elements will allow you to achieve a beautiful backlit portrait. And the interesting backdrops they create will be a bonus!

The following links with instructions do not intend to replace the included manufacturer's documentation. They aim to help customers who cannot benefit from our setup service, to achieve a smooth installation and a quick start. How to assemble the microscope? How to apply immersion oil with the 100X oil immersion lens? How to attach the microscope camera?

When shooting with a backlight, the (main) light source is often positioned behind the (primary) subject. This is in contrast to a standard lighting scenario where the light source is placed behind the camera in the direction of the subject. Whereas the golden glow that you might notice at the back or edges of the subject formed due to placing the primary subject between the main light source and the camera indicates that backlighting is what creates this effect.

Transmission electronmicroscopy

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Type of microscope

As is the case in any type of photography, the importance and need of this step stays true for backlighting as well. Start out with a few trial and error shots, get those test shots and carefully analyze them for improvement. Once you get the lighting and the settings right, try to compose and take some safe shots that you know will work. But once you have a couple of safe shots, do not hesitate to experiment and try out different angles for more variation. There is always a scope for creative curation and improvement. Moreover, different camera angles could help you add different perspectives to your scene and subject.

Although backlighting can be difficult to master at first, it is a helpful skill to have for a photographer. Moreover, experimenting with different backlighting scenarios and settings can help you yield different results. And in the points listed below, we walk you through some of those scenarios.

No - alone for the development and production of a single component of the microscope as its objective needs manufacturers systems with clean rooms and staff of several dozen people. We sell microscopes and microscope cameras from renowned manufacturers under the original manufacturer name. We sure combine microscopes, adapters and cameras from different manufacturers to achieve the best result, but never engage technically into the product - that is the task of the manufacturer who specializes in this task. It may be that there are traders who buy microscopes of a manufacturer, then modify them technically and then sell them as their own product under a different name (often the dealer's name or a fictitious name) - in this case, you have no guarantee from a renowned manufacturer but depend on the dealer because he is the only who "knows" this product.

Free technical support during the choice of the appropriate combination of devices. We do not "only" sell these products but we have thoroughly tested almost every offered combination. Further we have many years of experience and lots of successful setups at several customers in Austria, Germany and Switzerland. You can take advantage of our experience and have a free technical consultation. Product Demonstration and Training: By appointment you can test the offered dark field sets in our showroom in Vienna and assess the differences in image quality and ease of use of the software itself. You can also book a crash-course for the technical usage of the microscope and the cameras. Free additional documentation: Customers who cannot benefit from our setup service (mostly because of the geographical distance), receive a printed manual (in addition to the manufacturer's instructions) and video instructions (e.g. for the assembly of the microscope) which both help to have a smooth and quick start.

Achromatic microscope objectives have in comparison to other types of objectives the simplest optical structure and the lowest price level. One drawback is that they have a field curvature and therefore find application mainly in simpler laboratory microscopes as well as for routine tasks when the remaining field curvature does not interfere. Planapochromatic microscope objectives have achromatic or apochromatic correction, where in particular, the curvature of field for an intermediate image of medium size (normal field) is corrected. Modern plan objectives have a flattened image field of 20 to about 25 mm in diameter. They are used in laboratory and routine microscopes and in the micro photography.

Dark field microscopy

Multiple factors go into creating images that stand out. And one of them is lighting. It’s important to ensure that you have the right lighting to deliver stunning images to your clients. When used creatively, lighting has the power to elevate your images from good to outstanding. On the other hand, poor lighting could, in fact, ruin an otherwise well-composed image. Think of harsh lighting and washed-out photos! When shooting your subjects directly in front of the light, backlighting is a technique that you can use to create quality images. For your help, we’ve shared the guide to explain what is backlighting in photography and how to create stunning backlit images!

When choosing our suppliers for dark field microscopes we followed the needs of our customers and considered the following factors: Brand: By choosing a reputable manufacturer like OPTIKA, a level of quality and the technical and warranty support are guaranteed – contrary to "noname" products. Image quality: The B500-TDK is the top model of OPTIKA in the area of biological dark field microscopes. Meanwhile, the satisfaction of our customers has repeatedly confirmed this. State of the art: LED technology is integrated within the device - so no halogen lamp replacement and no loud cooling fans for halogen lamp cooling is needed. High quality 100X lens with a built-in iris. Plan-achromate optics for a sharp image over the entire field of view. Extensibility : With optional adapters, microscope cameras and SLR cameras can be connected - even microscope cameras from other manufacturers. Price-Performance ratio: With the B500-TDK (but also with the recently introduced lighter version B383-DK), we are convinced that we can offer the best price/performance ratio for the requirements of our customers. Training and Support: We can assemble the B500-TDK for you on-site, calibrate and train you in the calibration / usage - this service is highly recommended for customers who have never worked with a dark field microscope before. Technology upgrades: Should after a few years of the technology state of the art change, we offer to our customers targeted upgrades. We offer e.g. to exchange your older microscope camera model (bought from us) against the latest HDMI cameras - many of our customers already benefit from this.

As a photographer, you are not always shooting outdoors or during the daytime, and even if you are, it might not always be a sunlit day. In moments like these, you can seek the help of artificial lights to recreate the phenomenon of backlighting.

to have a down on so./sth. [ coll. ] (Brit.).

Yes - the software of our cameras can more than "just" the live image and save photos. Some camera software for example, shots every prefined time a photo and thus document the decay. Other software modules can make comparisons or counting. Just let us know if you need something special!

We have said this earlier, and we’ll say it again - make the flash a staple in your camera bag. We have covered several ways to use off-camera flash in previous blog posts, and when it comes to backlighting images, flash can be used in a variety of ways. Not only can it help add light to your subjects when the backlight is very strong, but an off-camera flash can also help CREATE backlight for your images!

Having the correct camera settings in place can help you make the most of the scene. And in backlighting, due to the position of the light source, the subject’s front might appear a bit darker than the background itself. Which might require you to have to overexpose the scene slightly. To help you gain the desired effect, set the aperture to a wide f-stop (anywhere between f/2.8 to f.5.6). Experiment with the shutter speed by adjusting it between 1/100 and 1/640, all the while, setting the ISO to a standard 100. However, note that as much as these settings might help you shoot better with a backlight, they are not absolute values. Always be open to experimenting and change as and when needed, depending on the situation at hand.

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How you end up positioning your subject, camera, and lighting determines everything. Place your camera in the direction of the subject and position your subject in a way so that the backlight is directly behind the subject. This will help create a light spill effect, where the light seems to spill from behind the outline of your subject. Moreover, if you are adding any center lighting to direct some light to your subject’s face, keep the source hidden in front of the subject.

Differential interference contrastmicroscopy

Lightfield microscopy

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For you to continue to grow as a photographer and diversify your portfolio, mastering lighting techniques is a must. And a sound understanding of backlight photography could help you shoot under challenging lighting conditions and tackle harsh light. If you think that you do not have enough time to learn another lighting technique, we’d recommend you to identify areas of your business that don't need your immediate attention, such as your photography post-processing or album design, and outsource those tasks. This would free up your time and give you back the hours that you can spend mastering lighting of all kinds!

If you have never worked before with a dark field microscope, you will need definitely to understand the process of condenser centering and learn to work with the immersion oil. During on-site installations we will train you, if you order online we provide you with a printed manual (in addition to the manufacturer's instructions).

When the sun is closer to the horizon, the glow permeates the landscape amid which you shoot your couple. As you plan engagement shoots or wedding day photography, try to include the golden hour in your schedule so that you can capture images that bask in the golden hues.

To achieve that, all you have to do is replace the sun as the main light source with an artificial light source. One of the simplest ways to do this is to place a strobe just behind the subject at some distance. Now to add variation to this, you can place the backlight and the key light in a way so that it creates a 45-degree angle. But more importantly, keep experimenting with the position of the backlight in order to yield different results.

In addition to the quality of the lens, one should note if the adapter has a fixed or variable focal length. The former are chaper. With the ones with variable focal length you can focus the image through the eyepieces and then by varying the focal length of the adapter also on the screen and get both images sharp at the same time. This adjustment you make only once. With a fixed focal length you have almost always a variation and you always have to readjust with the fine course, if you change from the eyepieces to the screen, which is annoying. If you work only with the screen image, then the fixed focal length is the better choice as it is more economic.

That depends on your application. If you carry out investigations according to Dr. Enderlein's method, you need the lenses 10x, 40x and 100x (with total magnification 100x, 400x and 1000x). The 10X objective is used for the centering of the condenser. The actual viewing is done with the 40x and 100X lenses.

Generally one can say that the number of pixels is to be adjusted to your display size. At a 19" screen (native resolution of 1280x1024 pixels) a 1.3 megapixel camera is also sufficient. In our demonstration room we use as a 27 "screen in full HD resolution - that is 1920x1080 pixels, so 2.07 megapixels. Should we use there a 10 megapixel camera, the monitor can still show no more than 2.07 megapixels. Moreover it comes with the USB transfer in the case of cameras with high megapixel number, all this data volume is passed through the USB channel making the frame rate smaller. So you can reach for example, with Optikam B9 (10 megapixel camera) a maximum of 3 frames/second while usieng a camera with 3 megapixels provides more than twice that speed. For the purposes of darkfield you need a fast image, since you watch also moving objects!

Phase contrastmicroscopy

Initially, backlighting in photography can seem like a bit of a challenge! But applying these tips and continuous practice could help you get it right!

Before you even begin shooting with the backlight, switch your camera settings to the manual mode, rather than automatic. This will help you gain more control over adjusting the exposure. Sometimes, in harsh lighting situations, your camera can have difficulties in knowing where to focus, and what you might end up with is a blurry image. Moreover, backlighting in photography could be tricky to get right when the camera is set to the automatic mode. By default, the Auto mode is set to adjust the settings in order to lead to a perfectly well-lit image, which, in turn, could result in overexposing your backlit images. Therefore, switching to the manual mode will help you gain more creative control over the camera settings.

In natural settings, the sun acts as your primary light source. And when you position your subject against the sun with the camera facing the subject’s front, it acts as a natural backlight. You can further reflect the natural light to brighten the subject’s face with a soft ambient light. As we said before, the golden hour provides the perfect window after sunrise or before sunset for incorporating beautiful backlit portraits into your portfolio.

2) the outline or pose of the subject. The more spaced out and clearly defined the outline of the subject, the more striking the final image would turn out to be. Therefore, you could ask your subject to spread out or stretch a bit to create some gap between their legs and arms. This is done so that you do not end up with a black block instead of a creative silhouette. You could put the silhouette backlighting technique to use for a variety of subjects such as animals, architecture, and so on.

While backlit images can be achieved without a flash - allowing the natural light to give a soft glow or hazy look to the entire image - if, however, your signature style calls for a more dramatic image or perhaps, there is a lack of a strong backlight source, this is where a flash can really save the day! By placing one in front of your subjects, you will still retain the glow from the sun, but at the same time, the additional light from the flash will ensure that your subjects look more well-defined.

Spot metering allows the camera to focus on a set spot and, therefore, expose it as best as possible. This function is available on most of the high-quality professional DSLR cameras. Spot metering is perfect for backlighting, and with a bit of practice, it can become really simple! Shooting in light that is less than ideal can create an issue when it comes to properly exposing your subjects. Use spot metering to bring the focus to your subject and expose the scene for the subject than the background. If, for instance, your subject is a person’s face, try setting up the focus close to the subject’s eyes.

Of course you can use stronger eyepieces and thus reach higher magnifications than 1000x, but you should know that there are physical limits to the magnification and such ones would be "empty magnifications", that is, you no longer gain more optical information (a good comparison is the optical and digital zoom of a photo camera). You can trust that the manufacturers of microscopes invested much time and money in the development and if technically possible and useful, they would have already have offered such magnifications.

LINE camera stuns by having great filters, editing tools, and other ways to create a really awesome photo.