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A microscope objective may have a very small working distance of less than 1 mm in cases with large magnification. That can be inconvenient with some kind of ...
Depthof field
Depth of field refers to the area in front of and behind the subject that appears in focus. Put another way, it denotes the sharpness range either side of a focus point. You can adjust this range of sharpness by adjusting the aperture, the focal length, and the distance between the subject and the camera.
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Learning how to adjust the depth of field enables you to create a wide range of effects and styles with the camera. By controlling the aperture, the focal length, and the distance between the subject and the camera, you can alter the depth of field to achieve your desired result and create beautiful, impactful images. To learn more about depth of field, download our free 90-page eBook, An Introduction to Photography.
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Another factor affecting depth of field is the focal length of your lens. A longer focal length will result in a shallower depth of field, while a shorter focal length will increase the depth of field. This means that if you want to create a portrait with a blurred background, you should use a longer focal length. On the other hand, if you want to capture a landscape with everything in focus, you should use a shorter focal length.
The aperture is the opening in the lens through which light passes into the camera. As well as controlling the amount of light that enters the camera, adjusting the aperture also affects the depth of field. A wider aperture (lower f-number) will result in a shallower depth of field. A shallow depth of field means a smaller sharpness range. A narrower aperture (higher f-number) gives a greater depth of field, which leads to a larger sharpness range. If you want to isolate your subject and create a blurred background (for example, to create a bokeh effect), you should use a wide aperture for a shallow depth of field. If you want to keep everything in sharp focus, you should use a narrow aperture for a greater depth of field.
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microscope are determined by the numerical aperture (N.A.) of the objective. The N.A. is defined as the sine of half the angle of the cone of light from ...
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You can also alter the depth of field by changing the distance between the subject and the camera. The closer the subject is to the camera, the shallower the depth of field. Similarly, the farther away the subject is, the deeper the depth of field. To create a macro shot with a shallow depth of field, you should get your camera as close as possible to your subject. If you want to capture a landscape with everything in focus, you should stay farther away from the scene.
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