This combination ensures that the fluorescence microscope can efficiently excite the fluorophores and capture the resulting fluorescence with high specificity and sensitivity, leading to clear and detailed images of the sample.

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The excitation filter plays a crucial role in the operation of a fluorescence microscope. Its primary function is to selectively allow only specific wavelengths of light to pass through, which are necessary for exciting the fluorophores in the sample being observed. This process is fundamental to fluorescence microscopy, enabling the visualization of structures or molecules within a specimen that are otherwise invisible under normal light conditions.

In a fluorescence microscope, the excitation filter is part of an optical assembly that also includes a dichroic mirror (or beamsplitter) and an emission filter. Here is how they work together: