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The phase-contrast microscope operates on the basis of the fact that when light passes via a transparent part of a specimen, it travels slower and tends to be shifted compared to the uninfluenced light.
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However, this difference in phase is not visible to the human eye. A transparent phase-plate in the phase-contrast microscope assists in increasing the change in phase to half a wavelength, thus causing a difference in brightness, and leading to the transparent object shining out in contrast to its surroundings.
High-contrast images of transparent specimens, such as micro-organisms, thin tissue slices, living cells in culture, latex dispersions, lithographic patterns, glass fragments, and sub-cellular particles, like nuclei and organelles, can be viewed in detail.
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For instance, cilia and flagella can be viewed with bright field microscopy, but the sharp contrast can only be seen via a phase-contrast microscope. Likewise, clear details of the amoebae can be seen via a phase-contrast microscope.
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The phase-contrast microscope has the ability to show components in a cell or bacteria, which would otherwise be very difficult to see in a basic light microscope.
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In biological and medical research, transparent and colorless components in a cell can be clearly studied by using the phase-contrast microscope. Cell division is no longer a mystery, as the phase-contrast microscope can be used to examine the whole process in detail.
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…Bell Labs built the first gas laser, which generated a continuous infrared beam from a mixture of helium and neon. In 1962 Robert N. Hall and coworkers at the General Electric Research and Development Center in Schenectady, New York, made the first semiconductor laser.
A phase-contrast microscope is a type of light microscopy that intensifies contrasts of transparent and colorless objects by influencing the optical path of light.
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In cases where high magnifications are needed, and the specimen is colorless or the color of the fine details of the specimen does not show up well, the phase-contrast microscope is the ideal choice compared to bright field microscopy.
The idea of phase-contrast microscopy was first described in 1934 by Dutch physicist Frits Zernike. Upon realization of the importance of the phase-contrast microscope, Zernike was awarded the Nobel Prize in Physics in 1953.
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