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Two major lens components—the objective lens and the ocular lens, or eyepiece—work together to project the image of the specimen onto a sensor. This may be the human eye or a digital sensor, depending on the microscope setup.

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At Shanghai Optics, we design and manufacture custom objectives and imaging systems to support our customers’ needs in many industries, including medical, biomedical, machine version, scientific research, and metrology, etc. Taking the client’s budget and precision requirements into consideration, our experienced engineering team ensure that each design can be manufactured at a reasonable cost and the optical performance is being met based on fabrication, assembly, and alignment tolerance analysis.

The ocular lens, located at the top of a standard microscope and close to the sensor (receiving eye) receives the real image from the ocular lens, magnifies the image received and relays a virtual image to the sensor. While most eyepieces magnify 10x, there are some which provide no magnification and others which magnify as much as 30x. The magnification power of the microscope can be calculated by multiplying the magnification power of the eyepiece, or ocular lens, by the magnification power of the objective lens. For example, an objective lens with a magnification of 10x used in combination with a standard eyepiece (magnification 10x) would project an image of the specimen magnified 100x.

Objectivelens magnification

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

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To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

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MicrometerThis product may not be available in your area.View ProductMPLAPON Our MPLAPON plan apochromat objective lens series provides our highest level of chromatic correction and resolution capability, along with a high level of wavefront aberration correction. View ProductMPLAPON-Oil Our MPLAPON-Oil objective is a plan apochromat and oil immersion lens that provides our highest level of chromatic correction and resolution capability. The numerical aperture of 1.45 offers outstanding image resolution. View ProductMXPLFLN MXPLFLN objectives add depth to the MPLFLN series for epi-illumination imaging by offering a simultaneously improved numerical aperture and working distance. View ProductMXPLFLN-BD MXPLFLN-BD objective lenses add depth to the MPLFLN series for epi-illumination imaging by offering simultaneously improved numerical aperture and working distance. View ProductMPLN Our MPLN plan achromat lens series is dedicated to brightfield observation and provides excellent contrast and optimal flatness throughout the field of view. View ProductMPLN-BD Our MPLN plan achromat lens series is designed for both brightfield and darkfield observation and provides excellent contrast and optimal flatness throughout the field of view. View ProductMPLFLN The MPLFLN objective lens has well-balanced performance with a semi-apochromat color correction, a fair working distance, and a high numerical aperture. It is suitable for a wide range of applications. View ProductMPLFLN-BD The MPLFLN-BD objective lens has semi-apochromat color correction and suits a wide range of industrial inspection applications. It is specially designed for darkfield observation and examining scratches or etchings on polished surfaces. View ProductLMPLFLN Our LMPLFLN lens is part of our plan semi-apochromat series, providing longer working distances for added sample safety and observation with increased contrast. View ProductLMPLFLN-BD Our LMPLFLN-BD brightfield/darkfield objective lens is part of our plan semi-apochromat series, providing longer working distances for added sample safety and observation with increased contrast. View ProductSLMPLN The SLMPLN plan achromat objective lens offers an exceptionally long working distance and the image clarity that you expect from the Olympus UIS2 optical system. It is ideal for electronic assembly inspection and other similar applications. View ProductLCPLFLN-LCD The LCPLFLN-LCD objective lenses are optimal for observing samples through glass substrates, such as LCD panels. The adoption of optical correction rings enables aberration correction according to glass thickness. View ProductLMPLN-IR/LCPLN-IR Our LMPLN-IR and LCPLN-IR plan achromat lenses have a long working distance and are specifically designed for optimal transmission in the near-infrared region (700–1300 nm wavelengths). View ProductWhite Light Interferometry Objective Lens This objective lens is designed for the Mirau style of white light interferometers and maintains a high level of temperature tolerance. The optimized numerical aperture of 0.8 provides improved light gathering, with a working distance of 0.7 mm. View Product

The optical aberration correction determines the optical performance of an objective lens and plays a central role in the image quality and measurement accuracy of imaging or microscopy systems. According to the degrees of the aberration corrections, objective lenses are generally classified into five basic types: Achromat, Plan Achromat, Plan Fluorite (Plan Semi-Apochromat), Plan Apochromat, and Super Apochromat.

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Objectivelens microscope

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Since the objective is closest to the specimen being examined, it will relay a real image to the ocular lens. While doing so, it contributes a base magnification of anywhere from 4x (for a scanning objective lens, typically used to provide an overview of a sample) to 100x (for oil immersion objectives).

Important specifications are marked on the barrel of the objective, so students or researchers can easily identify the properties of an objective and determine the optical performance and working conditions for proper use. Figure 1 shows a diagram of an objective lens. A detailed discussion of the objection specifications is provided below.

Objectivelens microscope function

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While the simplest of microscopes is simply a magnifying glass with a single lens, compound microscopes used today are highly complex devices with a carefully designed series of lenses, filters, polarizers, beamsplitters, sensors, and perhaps even illumination sources. The exact combination of optical components used will depend on the application of the microscope; the wavelength of light with which it is intended to be used, and the resolution and magnification required in the final image.

High powerobjectivelens

Most objectives are designed to image specimens with air as the medium between the objective and the cover glass. However, for achieving higher working numerical apertures, some objectives are designed to image the specimen through another medium such as special oil with a refractive index of 1.51.

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Microscope Objectives or Objective lenses are in many ways the heart of the microscope, and are typically mounted on a rotating nosepiece or turret to enable easy selection. Many microscopes will be equipped with a scanning objective (4x), a low power objective (10x), a high power objective (40x), and perhaps even an oil immersion objective lens.

Objectives are complex multi-element lenses. For any given application, careful consideration of the optical parameters and specifications is necessary. In many cases, custom-designed objective assemblies provide the best-fit solution for meeting all the requirements of a specialized application.  Custom parameters may include antireflection coatings, chromatic focus shift, working distance, image quality (MTF and spot size), lens mount, glass window thickness, and field of view, among others.

Magnification is one important parameter. Magnification is usually denoted by an X next to a numeric value.  Objectives are available in a range of magnifications from 2X to 200X.

Olympus microscope objective lenses for industrial inspections offer outstanding optical performance from the visible light to near-infrared region. At Evident, we offer an extensive selection of Olympus objectives suited to specific inspection requirements and tasks. Our MXPLFLN-BD objective is designed for darkfield observation and examining scratches on polished surfaces, while our SLMPLN objective is ideal for electronic assembly inspection. Find your ideal microscope objective today for your inspection task. No matter your requirements, Olympus objective lenses have you covered.

Objectivelens function

Field of View is the area of the object that can be imaged by a microscopy system. The size of the field of view is determined by the objective magnification or focal length of the tube lens for an infinite-corrected objective. In a camera system, the field of view of the objective is related to the sensor size.

Many microscopes have several objective lenses that you can rotate to view the specimen at varying magnification powers. Usually, you will find multiple objective lenes on a microscope, consisting of 1.25X to 150X.

MXPLFLN-BD objective lenses add depth to the MPLFLN series for epi-illumination imaging by offering simultaneously improved numerical aperture and working distance.

The parfocal length is the distance between the objective mounting plane and the specimen / object. This is another specification that can often vary by manufacturer.

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Since indirect backlight illumination is generally more effective than direct illumination, most microscopes do not include an internal light source.  Instead, they rely on daylight or on background illumination such as a lightbulb. In brightfield illumination, also known as Koehler illumination, two convex lenses saturate the specimen with external light admitted from behind. These two lenses, the collector lens and condenser lens, work together to provide a bright, even, and constant light throughout the system: on the image plane as well as on the object plane.  This system of illumination is used in many compound microscopes, including student microscopes and those found in many research labs.

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A microscope objective is an important component of a microscopy or imaging system for a range of science research, biological, industrial, and general lab applications.. An objective lens determines the basic performance of an optical microscope or imaging systems and is designed for various performance needs and applications. It is located closest to the object and is an important component in imaging an object onto the human eye or an image sensor.

Each microscope objective is itself a complex assembly of lenses, and besides contributing to the magnification, it is the objective lens which determines the resolution power of the microscope. An objective lens can also provide optical aberration corrections.  A reflective objective, for instance, includes two mirrors within the assembly. These mirrors can focus laser light as well as provide chromatic corrections.

Many objectives are designed to be used with a cover glass. Using an incorrect coverslip thickness can greatly reduce the optical performance of a microscopy system.

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

where θ is the maximum 1/2 acceptance ray angle of the objective, and n is the index of refraction of the immersion medium. Figure 2 shows the ray angle θ of an infinity-corrected objective.

A microscope is a special optical device designed to magnify the image of an object. Depending on the type of microscope, it may project the image either onto a human eye or onto a recording or video device. As an example, consider the photographs of cells that can be found in a science textbook. These photographs have all been taken by a specialized microscope, and may be called micrographs.

Types ofobjectivelenses

MXPLFLN objectives add depth to the MPLFLN series for epi-illumination imaging by offering a simultaneously improved numerical aperture and working distance.

A simple magnifier (magnifying glass), works when the object to be examined is situated within focal length of the magnifier lens, enabling larger virtual image is produced. This type of magnifier is very limited in both resolution and magnification. A compound microscope, on the other hand, uses a relay lens system instead of the single lens, and since each lens component can contribute magnifying power, the result is greatly increased capability.

For keeping the objective at the proper position, there are mounting threads on almost all objectives. Commonly used mounting threads include RMS, M25 x 0.75, M26X 0.706, M32 x 0.75.

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The ocular lens, or eyepiece, is also an optical assembly rather than a single lens, but it is typically more simple than the objective. Often it is composed of two lenses: a field lens and an eye lens. The design of the ocular lens determines the field of view of the microscope, as well as contributing to the total magnification of the system.

Objective lenses can be classified based on the objective construction, field of use, microscopy method, performance (optical aberration corrections), and magnification. Many microscope objective manufacturers offer a wide range of objective designs, which provide various degrees of optical aberration corrections for supporting different needs. Mirrors or reflective elements are used in objective lenses for the applications that requires chromatic aberration over board spectral ranges. Most traditional microscopy systems use refractive objectives such as achromatic objectives (the cheaper objectives) for laboratory microscope applications and plan apochromats (expensive objectives) for biological and science research microscope applications.