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Most people use put a transparent plastic ruler under microscope to perform this task. Actually, the microscope itself provides pretty accurate measurements ranging from 0.2 - 10 mm. Based on optical physics, the diameter of the field of view can be reliably derived by a simple formula:
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Focal Length Calculator · Effective Focal Length, EFL (mm): · Back Focal Length, BFL (mm): · Front Focal Length, FFL (mm): · Primary Principal Point, P (mm): ...
For example: Diameter of the field of view (mm) = 20 / 40 = 0.50, where 20 is the field number of eyepiece, and 40 = objective mag.
Type-B plugs are typically at one end of a USB A/B cable. The USB Type-B plug fits into the Type-B receptacle on the printer or another device, while the USB Type-A plug fits into the type A receptacle located on the host device, like a computer.
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USB Type-B connectors, officially called Standard-B connectors, are square with slight rounding or large square protrusion on the top, depending on the version.
Field of view microscope10X
Most modern microscopes have eyepieces with the number of field of view at 20 or 22. The diameters of the field of view are listed:
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Field of view microscope40x
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Apart from USB4, every USB version supports Type-B connectors, including USB 3.0, USB 2.0, and USB 1.1. The second type of "B" connector, called Powered-B, also exists, but only in USB 3.0.
Diameter of the field of view (mm) = F / M, where F is the number of field of view (FOV) of the eyepiece, and M is the magnification (mag.) of the objective.
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The Type-B connectors in USB 2.0 and USB 1.1 are identical, meaning that the USB Type-B plug from one USB version will fit into the Type-B receptacle from both its own and the other USB version.
Size, mostly. The Type-B connector is large compared to, for example, USB Type-C, which you'll more often see on devices today.
USB Type-B receptacles are often on larger computer devices like printers and scanners. Sometimes, you'll find Type-B ports on external storage devices like optical drives, floppy drives, and hard drive enclosures.
There are two USB 3.0 Type-B connectors, USB 3.0 Standard-B and USB 3.0 Powered-B. The plugs and receptacles are identical in shape and follow the outlined physical compatibility rules. However, USB 3.0 Powered-B connectors have two additional pins to provide power, for a total of eleven.
Field of view microscopeformula
USB 3.0 Type-B connectors are often blue, while USB 2.0 Type-B and USB 1.1 Type-B connectors are often black. That's not always the case because the manufacturer can choose any color for USB Type-B connectors and cables.
In other words, USB 1.1 and 2.0 Type-B plugs are physically compatible with USB 3.0 Type-B receptacles, but USB 3.0 Type-B plugs aren't compatible with USB 1.1 or USB 2.0 Type-B receptacles.
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The fact that a Type-B connector from one USB version fits in the Type-B connector from another USB version doesn't imply anything about speed or functionality.
USB 3.0 Type-B connectors have nine pins, several more than the four pins found in previous USB Type-B connectors, to allow for a faster USB 3.0 data transfer rate. Those pins had to go somewhere, so the Type-B shape had to be altered somewhat.
Field of view microscopeCalculator
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Polaroid sunglasses are familiar to most of us. They have a special ability to cut the glare of light reflected from water or glass (see Figure 1).
USB 3.0 Type-B connectors are a different shape from previous ones, so the plugs don't fit in those receptacles. However, the USB 3.0 Type-B form factor was designed to allow previous USB Type-B plugs from USB 2.0 and USB 1.1 to fit with USB 3.0 Type-B receptacles.
The field area (A) is calculated by . If the cell is used as unit (instead of mm), the total number of cells in the field of view can be derived from the number of cells on the diameter line with the formula. This is an excellent approximation for most of our histological samples. For example, if 16 positive cells and 28 total cells are on the line across diameter, respectively. Then Positive cells (Pi x 82 =201) divided by total cells number (Pi x 142 = 616) in entire field would be % of positive cells (201/ 616= 32.6%) (Figure).