What Is Depth Of Field Microscope ? - what is the depth of field on a microscope
Jun 27, 2024 — Near-infrared (NIR) sensors capture and analyze light within the 900 to 1700 nm wavelength range, which is beyond visible light, ...
Achromatic color scheme
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Achromatic meaning
The lenscalculator calculates the Focal length of the lens, based on the desired Field of View (FOV), working distance (WD) and image sensor size.
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A solar god of Egyptian mythology, usually depicted with a falcon's head.... Click for English pronunciations, examples sentences, video.
How to pronounce achromatic
Achromatic color
An objective lens determines the basic performance of an optical microscope or imaging systems and is designed for various performance needs and applications.
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Achromatic color meaning
Achromatic in art
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Describing a lens consisting of two or more optical components (elements), intended to correct for chromatic aberration. Commonly used as the objective of small refractors, the achromatic lens (or achromat) was invented in 1729 by the English optician Chester Moor Hall (1703–71) and first manufactured commercially by J. Dollond in 1758. It has one element of crown glass and another of flint glass. The dispersion (1) of the crown glass compensates for the chromatic error of the flint glass, while still leaving some refractive power. The two-element design is termed an achromatic doublet. It is practically impossible to correct all wavelengths of light, however, and most lenses adopt a compromise, bringing two particular wavelengths to a common focus, thus reducing the false colour. A lens that corrects for more than two wavelengths is termed an apochromatic lens.
Achromatic lens
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The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has provided a myriad of applications for biological systems. Over the last several years, mutagenesis studies have improved folding properties of GFP (refs 1,2). However, slow maturation is still a big obstacle to the use of GFP variants for visualization. These problems are exacerbated when GFP variants are expressed at 37 degrees C and/or targeted to certain organelles. Thus, obtaining GFP variants that mature more efficiently is crucial for the development of expanded research applications. Among Aequorea GFP variants, yellow fluorescent proteins (YFPs) are relatively acid-sensitive, and uniquely quenched by chloride ion (Cl-). For YFP to be fully and stably fluorescent, mutations that decrease the sensitivity to both pH and Cl- are desired. Here we describe the development of an improved version of YFP named "Venus". Venus contains a novel mutation, F46L, which at 37 degrees C greatly accelerates oxidation of the chromophore, the rate-limiting step of maturation. As a result of other mutations, F64L/M153T/V163A/S175G, Venus folds well and is relatively tolerant of exposure to acidosis and Cl-. We succeeded in efficiently targeting a neuropeptide Y-Venus fusion protein to the dense-core granules of PC12 cells. Its secretion was readily monitored by measuring release of fluorescence into the medium. The use of Venus as an acceptor allowed early detection of reliable signals of fluorescence resonance energy transfer (FRET) for Ca2+ measurements in brain slices. With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before.
Extreme ultra-violet (EUV) light has many realised and potential applications. For example, the fastest computers chips are now processed using EUV light to ...
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