The laser power level used is generally considered safe but laser safety glasses for laser light at 1040nm nm and 1550 nm will be available.

If you agree, we may use your personal information from any of these Amazon services to personalize the ads we show you on other services. For example, we may use your Prime Video Watch history to personalize the ads we show you on our Stores or on Fire TV. We may also use personal information we receive from third parties (like demographic information).

Holoor

Material: H-K9 optical glass. And the spectral ratio is R:T=50%:50% Dimensions: Tolerance: ± 0.1 mm. Surface quality: 60/40. Wavelength (nm): 400 ------ 700

Customer Reviews, including Product Star Ratings, help customers to learn more about the product and decide whether it is the right product for them.

Skills that will be acquired during this Immersion include optical design and alignment, data acquisition and processing, and familiarity with multiphoton microscopy.

Participants will learn the design and construction of a multiphoton microscope along with all the required data acquisition and signal processing steps. A special nonlinear knife-edge technique will be applied to measure the resolution of the microscope in the x, y and z directions. Different nonlinear optical effects will be investigated to create an image including SHG, THG and multiphoton excitation fluorescence. In particular, three-photon excitation fluorescence from chlorophyll in a fresh leaf will be observed. Other prepared biological samples (mouse brain section, various fixed tissues of different diseases, etc.) will be also available for experimenting. Advanced imaging techniques such as tiling/stacking will be introduced if time allows. A sensitive spectrometer will also be available to acquire the optical spectrum of the nonlinear optical emission from samples.

(a) Schematic diagram of the multi-photon microscope. (b) Photograph of the microscope. Examples of a multiphoton images are shown in (c) the end of a mosquito needle and (d) a full cross-section of a mouse brain. Click on the image to see a larger image. A fuller description of the apparatus appears below.

To calculate the overall star rating and percentage breakdown by star, we don’t use a simple average. Instead, our system considers things like how recent a review is and if the reviewer bought the item on Amazon. It also analyses reviews to verify trustworthiness.

We feature offers with an Add to Basket button when an offer meets our high quality standards for: Price,Delivery option, andCustomer service “No featured offers available” means no offers currently meet all of these expectations. Select See All Buying Options to shop available offers.

Beamshaper

A basic multiphoton microscope described here can be constructed for approximately $10,000 (not including the femtosecond fiber laser which is commercially available at the cost of ∼$10,000). A research grade microscope will cost around $30k using state-of-the-art new components that are commercially available. For comparison, a basic commercial multiphoton microscope would cost over $500,000.

In any case, we use cookies and similar tools that are necessary to enable you to make purchases, to enhance your shopping experiences and to provide our services, as detailed in our Cookie notice. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. Click “Decline” to reject, or “Customise” to make more detailed advertising choices, or learn more. You can change your choices at any time by visiting Cookies and advertising choices. To learn more about how and for what purposes Amazon uses personal information (such as Store order history or Prime Video Watch history) and cookies, please visit our Privacy notice and our Cookie notice.

Multi-photon microscopy (MPM) is a powerful technique that allows three dimensional mapping of samples that have a measurable nonlinear optical response such as second harmonic generation (SHG), third harmonic generation (THG) or fluorescence induced by multiphoton absorption. MPM provides us with a way to see the nonlinear microscopic world with high resolution, in 3D. After about two decades of intensive development MPM has found important applications in nonlinear materials characterization, biological research and in diagnosing medical conditions. Immersion participants will learn how to build a compact multiphoton microscope using a femtosecond fiber laser as the excitation source. Different nonlinear optical contrast mechanisms will be introduced. The optical and mechanical design of the microscope will be discussed. Participants will assemble the microscope from scratch, perform alignment, and acquire multiphoton images from various samples including biological tissues.

If you agree, we may use your personal information from any of these Amazon services to personalize the ads we show you on other services. For example, we may use your Prime Video Watch history to personalize the ads we show you on our Stores or on Fire TV. We may also use personal information we receive from third parties (like demographic information).In addition, if you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie notice. Your choice applies to using first-party and third-party advertising cookies on this service. Cookies store or access standard device information such as a unique identifier. The 96 third parties who use cookies on this service do so for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products.In any case, we use cookies and similar tools that are necessary to enable you to make purchases, to enhance your shopping experiences and to provide our services, as detailed in our Cookie notice. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. Click “Decline” to reject, or “Customise” to make more detailed advertising choices, or learn more. You can change your choices at any time by visiting Cookies and advertising choices. To learn more about how and for what purposes Amazon uses personal information (such as Store order history or Prime Video Watch history) and cookies, please visit our Privacy notice and our Cookie notice.

The most important application of MPM is in biological research and diseases diagnosis. Imaging technology has and will continue to influence medicine by advancing research and development (R&D) based on mechanistic insights into biomedical processes that ultimately lead to innovative pharmaceuticals, diagnostics to better plan treatment, and clinical management of diseases to improve outcomes. Immersion participants will learn about a couple of ongoing research efforts such as brain and cancer imaging. Nonlinear material and device characterization is another interesting application where MPM can play an important role. If time allows, participants will have a chance to image some interesting 2D materials such as graphene with the multiphoton microscope. The microscope can also be used for 3D writing which is an important emerging technique for creating advanced optical and medical components.

Found a lower price? Let us know. Although we can't match every price reported, we'll use your feedback to ensure that our prices remain competitive.

In addition, if you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie notice. Your choice applies to using first-party and third-party advertising cookies on this service. Cookies store or access standard device information such as a unique identifier. The 96 third parties who use cookies on this service do so for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products.

Participants are encouraged to bring a notebook. A laptop computer would be helpful to view, store and display multiphoton images. Please feel free to bring your own samples that you want to image with the multiphoton microscope.