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The microscope is one of the most iconic and commonly used tools in many scientific fields. We rely on these devices to observe things that are so small that they are otherwise invisible to the naked eye. To do this, the microscope makes use of both an ocular and an objective lens. If you don't know the difference, don't worry; this article will tell you everything you need to know about these two lens types and how they function together to make microscopes work.

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While it may initially seem redundant to have two separate lenses in your microscope, they do far more together than they ever could on their own.

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There are many other kinds of objective lenses out there, so you have no shortage of options. Do some research and find out which lens best suits your needs and goals.

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This is why a microscope is such a good investment for anyone interested in science. If you want to understand and examine the world around you, there's no better tool. AmScope's selection is built to last, and we carry all kinds of objective lenses as well, so a microscope from us will serve you well for many years.

There are four main types of objective lenses, each with a different diameter of field of view, and therefore a different magnification level:

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Often, your microscope will have at least three objective lenses on a rotating disc, each with a different magnification level. If you find your current lens lacking, it's easy to switch to one of the others. Objective lenses with higher magnification have shorter focal lengths, or less space between the lens and the surface of the subject. Since depth of field decreases as magnification increases, those wanting a broader field of view should stick to shorter lenses. For example, if your current objective lens has 100x magnification but you need a wider field of view, you'll want to switch to a lens with lower magnification, such as 40x.

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The objective and ocular lens are found on different parts of the microscope. The ocular lens is part of the eyepiece and therefore closer to your eye as you look into the microscope. The location of the eyepiece always indicates the correct observing position at or near the top of the microscope.

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The objective lens, on the other hand, looms over your subject, typically near the middle of the microscope. This is because the objective lens is responsible for gathering light reflections from your subject. It then shoots a beam of light into the microscope, which becomes an image that you observe from the eyepiece containing the ocular lens.

In contrast, your microscope's eyepiece will usually have only one ocular lens, though you can usually swap the eyepiece as well. The standard magnification level of the ocular lens is 10x, but there are stronger ones available. When selecting an eyepiece, you should think about eye relief, or the required distance between your eyes and the lens. Eyepieces with large eye relief give you some space, while those with small eye relief require you to be up close.

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Your objective lens isn't just for increasing the size of your subject; it can also provide better resolution. For example, achromatic lenses contain two smaller lenses (convex and concave) that are used to limit the refracting light of your subject, and phase-contrast lenses use phase plates to pick up miniscule changes in wavelength amplitude, making moving subjects easier to observe. Lenses like these help reduce ghost images so that the real image is projected to your eyepiece.

Everyone knows that microscopes are a crucial tool in science, but few realize how versatile and adaptable they can be. Thanks to the variance in lenses, microscopes can serve all kinds of purposes for all kinds of people, from the doctor identifying cancer cells to the child wanting to get a closer look at their favorite bug. Once you know how all of the optical elements work together, like the ocular lens vs objective lens, it's easy to maximize the efficiency of your microscope.

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Nov 23, 2021 — Your final choice will depend on factors like the overall size, detection limits, wavelength range, cost and measurement time.

Figuring out the total magnification power of your microscope is easy: just multiply the power of your objective lens by your ocular lens. For instance, if your eyepiece has 10x magnification and you're using a low-power lens (10x), you have 100x magnification in total. Switch to your scanning lens (4x), and magnification becomes 40x. It's important to keep in mind that the ocular lens and objective lens total magnification is ultimately what you're viewing. If you were viewing your subject through a single lens, then that lens would have to be extremely powerful to match what you can easily get with both. Therefore, one lens isn't nearly as effective without the other.

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