High power objective microscope function

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What is objective lens in microscope

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It is easy to think that more microscope magnification will result in seeing more detail and a better image. However, keep in mind that more magnification means you will see a much smaller area on your sample. Additionally if you get too much magnification (anything over 1000x), you will end up with empty magnification and poor resolution. So no, more microscope magnification is not necessarily better.

What is the shortest objective called

Objective lens microscope function

Contributions of scapulothoracic upward rotation (Upward Rot), re/protraction (RePro), and tilt to scapulothoracic-contributed…

Correlation between scapulothoracic-contributed (ST-contributed) axial rotation and glenohumeral (GH) plane of elevation (PoE).…

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Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample. Most microscope objective lenses are parfocal, meaning once you have one objective lens in focus, all other objectives should be in focus as well as you move from 4x to 10x, etc. Occasionally, when switching to the highest magnification lens you may need to make a slight adjustment to the fine focus, but this is not required when you start out with the 4x lens. So starting your microscopy viewing at the lowest objective is usually the most simple way to start. You can certainly use another magnification to begin, but it will likely take more time and can be a bit discouraging if you just can't seem to locate the area of your specimen you wish to view, especially if you are a microscopy novice.

Magnification of objectivesformula

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Magnification of objectivesin microscope

An important point to understand when working with compound microscopes and their objective lenses is that the field of view changes as the magnification changes. Typically a lower magnification objective lens will have a larger field of view, and a higher magnification objective lens will have a smaller field of view. For more information on how magnification affects the field of view read this article on microscope magnification and field of view.

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Magnification ofmicroscope

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Glenohumeral and scapulothoracic motion combine to generate humerothoracic motion, but their discrete contributions towards humerothoracic axial rotation have not been investigated. Understanding their contributions to axial rotation is important to judge the effects of pathology, surgical intervention, and physiotherapy. Therefore, the purpose of this study was to investigate the kinematic coupling between glenohumeral and scapulothoracic motion and determine their relative contributions towards axial rotation. Twenty healthy subjects (10 M/10F, ages 22-66) were previously recorded using biplane fluoroscopy while performing arm elevation in the coronal, scapular, and sagittal planes, and external rotation in 0° and 90° of abduction. Glenohumeral and scapulothoracic contributions towards axial rotation were computed by integrating the projection of glenohumeral and scapulothoracic angular velocity onto the humeral longitudinal axis, and analyzed using one dimensional statistical parametric mapping and linear regression. During arm elevation, scapulothoracic motion supplied 13-20° (76-94%) of axial rotation, mainly via scapulothoracic upward rotation. The contribution of scapulothoracic motion towards axial rotation was strongly correlated with glenohumeral plane of elevation during arm elevation. During external rotation, scapulothoracic motion contributed 10° (8%) towards axial rotation in 0° of abduction and 15° (15%) in 90° of abduction. The contribution of scapulothoracic motion towards humerothoracic axial rotation could explain the simultaneous changes in glenohumeral plane of elevation and axial rotation associated with some pathologies and surgeries. Understanding how humerothoracic motion results from the functional coupling of scapulothoracic and glenohumeral motions may inform diagnostic and treatment strategies by targeting the source of movement impairments in clinical populations.

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