THE VIRTUAL EDGE: Lab 1 Introduction to Microscopy - high power in microscope

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Occasionally, the viewing angle or objective viewing angle is given, e.g. 6°. The field of view and viewing angle can be easily calculated:

Field of viewhuman eye

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Very small fields of view give the impression of looking through a tunnel. There is a lot of black on the outside and a small image area in the middle. this undesired effect occurs on simple products and primarily on many zoom binoculars.

The field of view describes the area seen when looking through the optics – without moving them. For example, if you look at the edge of a forest through binoculars at a distance of 1,000 m and see an area covering 120 m, the binoculars have a field of view of 120 m/1,000 m.

The field of view depends on the magnification, i.e. in general lower magnification levels have a larger field of view than higher levels. Therefore, when comparing different binoculars you must compare those with the same magnification only.

Field of viewcamera

The field of view cannot be calculated from the pure type designation (e.g. 10 x 42) but are specified by the optical design. The size of the prism and the eyepiece in particular determine which image angles can pass through the binoculars. The lens diameter does not affect this it only influences the brightness.

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Wide angle binoculars, on the other hand, shine with very large fields of view that offer a wide overview and comfortable observation. The field of view is so large that the image circle in the eyepiece appears at an angle of 60° or higher. This angle at which the image is observed in the eyepiece, is the subjective or eye-side viewing angle. The 60° threshold that determines the predicate “wide-angle” is specified in an ISO standard. Is a field of view, e.g. 120 m at 1,000 m, good or bad for binoculars? The answer depends on the magnification. 10x binoculars with this field of view would be very good (subjective viewing angle approx. 69°), a 7x pair with this field of view would be rather weak (subjective viewing angle of 48° only). The subjective viewing angle shows immediately if the field of view is pleasantly large and comfortable or if a tunnel vision can be expected.

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

Human eyefield of viewin mm

Field of view definitionmicroscope

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To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Field of view definitionscience

Unlike binoculars or spotting scopes, riflescopes generally have a smaller field of view and it is given at 100 m instead of 1,000 m. For safety reasons (recoil), however, a complete view can be obtained from a much larger distance (eye relief).

Field of viewcalculator

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When comparing the edge definition of different binoculars, it is vital to not only use the same types (e.g. 10 x 42) only, but also take the fields of view into consideration. If one pair has a field of view of 120 m and another 100m at 1,000 m only, it is easier for the second to have better edge definition. However, this “benefit” comes with a considerable loss of field of view.

Many microscopes have several objective lenses that you can rotate to view the specimen at varying magnification powers. Usually, you will find multiple objective lenes on a microscope, consisting of 1.25X to 150X.

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Field of view definitionphotography

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

MXPLFLN-BD objective lenses add depth to the MPLFLN series for epi-illumination imaging by offering simultaneously improved numerical aperture and working distance.