Line pairradiology

Jun 12, 2019 — Most household detectors contain a very small amount of americium-241, a radioactive isotope. The slight amount of radiation that can be ...

Line Pair GaugeThanks Mr. Lopez for your quick response. The figure of gauge does NOT have clear instruction label which numerical scale is LP/mm and it placed on the middle so it’s created confusion and could be any of the two numerical scale. From your explanation I could understand the right numerical scale is LP/mm. Based on your explanation I could imagine if able to see 5 Individual Peaks in line profile (say at red line position) then my resolution is 3.5 lp/mm. Still not clear what is the purpose of left numerical scale and if their conversion from left to right. Most of the searched found documents lacks in clear practical instruction about its use and might assumed that they user already know how to use it.   Reply Translate  close  Rick Lopez R & D, John Deere - Tech Works Ames, USA, Joined Jul 2011, 220 17:01 Dec-15-2016 https://www.ndt.net/forum/thread.php?msgID=66274#66274 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 16:17 Dec-15-2016 . You are almost on the right track. A simple left-side to right-side horizontal line is not correct. The numbers on the right just tell the user what the horizontal increments on the left side are. For example, if your magnification system allows you to resolve as fine as the 17th increment down, then you would have 16 line-pairs per millimeter resolution.   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 18:07 Dec-16-2016 https://www.ndt.net/forum/thread.php?msgID=66286#66286 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 17:01 Dec-15-2016 . Thanks again Rick for clarification. My previous understanding was wrong. So its as you said "numbers on the right are horizontal increments value on the left side are". So need to get horizontal increment position from left scale (lowest position of line signal profile where still can see five distinct individual peaks) and corresponding numerical value (as per right scale) is LP/MM. So right is scale is just aiding to display the left increment value and you can still gauge LP/MM just using the left scale only. When I correlate left increment position to right number only one number still remain odd is number 11. I could not match with it any left horizontal increment. Correct me if I am wrong again. How about when you are in middle or somewhere between two horizontal increment? Assuming just visual interpolation of value. Thanks.   Reply Translate  close  Dr. Uwe Zscherpel Director, BAM Berlin, Germany, Joined Jan 2010, 127 128 11:24 Dec-20-2016 https://www.ndt.net/forum/thread.php?msgID=66304#66304 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 18:07 Dec-16-2016 . Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Line pairresolution

You are almost on the right track. A simple left-side to right-side horizontal line is not correct. The numbers on the right just tell the user what the horizontal increments on the left side are. For example, if your magnification system allows you to resolve as fine as the 17th increment down, then you would have 16 line-pairs per millimeter resolution.   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 18:07 Dec-16-2016 https://www.ndt.net/forum/thread.php?msgID=66286#66286 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 17:01 Dec-15-2016 . Thanks again Rick for clarification. My previous understanding was wrong. So its as you said "numbers on the right are horizontal increments value on the left side are". So need to get horizontal increment position from left scale (lowest position of line signal profile where still can see five distinct individual peaks) and corresponding numerical value (as per right scale) is LP/MM. So right is scale is just aiding to display the left increment value and you can still gauge LP/MM just using the left scale only. When I correlate left increment position to right number only one number still remain odd is number 11. I could not match with it any left horizontal increment. Correct me if I am wrong again. How about when you are in middle or somewhere between two horizontal increment? Assuming just visual interpolation of value. Thanks.   Reply Translate  close  Dr. Uwe Zscherpel Director, BAM Berlin, Germany, Joined Jan 2010, 127 128 11:24 Dec-20-2016 https://www.ndt.net/forum/thread.php?msgID=66304#66304 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 18:07 Dec-16-2016 . Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Our green laser pointers provide 100% IR filtration giving you peace of mind that it is safe to use for presentation and pointing. Used widely in classrooms, ...

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Pairof lines examples

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by L Feng · 2014 · Cited by 1743 — Using the PT symmetry–breaking concept, we delicately manipulated the gain and loss of a microring resonator and observed single-mode laser oscillation of a ...

Our parabolic reflectors are like steroids for 100W LED chips. They are much more efficient than glass lenses, directing ... TERMS AND CONDITIONS OF SALES.

Jun 24, 2022 — In summary, the Tract Toric Hunting Scope is a very nice, high quality, rugged, functional long range hunting scope. It tracks as it should, ...

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Good Day I am trying to understand the way to measure resolution using line pair gauge. Attached is one of the LP gauge available in web for radiographic image assessment. I could not find any literature explaining the number on left and number of right and their conversion relationship. Assuming my resolution is somewhere in the gauges where five distinct wires are separate visible. Please explain how to assess image resolution at some point in the gauge. Thanks in advance for your time and appreciate your support. Happy Holidays.   Reply Translate  close SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos  Rick Lopez R & D, John Deere - Tech Works Ames, USA, Joined Jul 2011, 220 23:08 Dec-14-2016 https://www.ndt.net/forum/thread.php?msgID=66255#66255 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 22:22 Dec-14-2016 (Opening). A quick Google search shows that some background info can be found here: https://www.nde-ed.org/EducationResources/CommunityCollege/Radiography/AdvancedTechniques/Real_Time_Radiography/ImageQualityIndicators.htm Horizontal lines on left in your photo correlate to increments of line pairs per millimeter resolution of system under test shown on the right (e.g. "I can see no better than the 5th horizontal line", so your resolution is the 5th value down on the right, or 3.5 LP/mm).   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 16:17 Dec-15-2016 https://www.ndt.net/forum/thread.php?msgID=66272#66272 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 23:08 Dec-14-2016 . Line Pair GaugeThanks Mr. Lopez for your quick response. The figure of gauge does NOT have clear instruction label which numerical scale is LP/mm and it placed on the middle so it’s created confusion and could be any of the two numerical scale. From your explanation I could understand the right numerical scale is LP/mm. Based on your explanation I could imagine if able to see 5 Individual Peaks in line profile (say at red line position) then my resolution is 3.5 lp/mm. Still not clear what is the purpose of left numerical scale and if their conversion from left to right. Most of the searched found documents lacks in clear practical instruction about its use and might assumed that they user already know how to use it.   Reply Translate  close  Rick Lopez R & D, John Deere - Tech Works Ames, USA, Joined Jul 2011, 220 17:01 Dec-15-2016 https://www.ndt.net/forum/thread.php?msgID=66274#66274 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 16:17 Dec-15-2016 . You are almost on the right track. A simple left-side to right-side horizontal line is not correct. The numbers on the right just tell the user what the horizontal increments on the left side are. For example, if your magnification system allows you to resolve as fine as the 17th increment down, then you would have 16 line-pairs per millimeter resolution.   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 18:07 Dec-16-2016 https://www.ndt.net/forum/thread.php?msgID=66286#66286 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 17:01 Dec-15-2016 . Thanks again Rick for clarification. My previous understanding was wrong. So its as you said "numbers on the right are horizontal increments value on the left side are". So need to get horizontal increment position from left scale (lowest position of line signal profile where still can see five distinct individual peaks) and corresponding numerical value (as per right scale) is LP/MM. So right is scale is just aiding to display the left increment value and you can still gauge LP/MM just using the left scale only. When I correlate left increment position to right number only one number still remain odd is number 11. I could not match with it any left horizontal increment. Correct me if I am wrong again. How about when you are in middle or somewhere between two horizontal increment? Assuming just visual interpolation of value. Thanks.   Reply Translate  close  Dr. Uwe Zscherpel Director, BAM Berlin, Germany, Joined Jan 2010, 127 128 11:24 Dec-20-2016 https://www.ndt.net/forum/thread.php?msgID=66304#66304 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 18:07 Dec-16-2016 . Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Mizuno, H. et al. Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol. Cell 12, 1051–1058 (2003).

Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems. Additionally, fluorescence microscopy is in a state of rapid evolution, with new techniques, probes and equipment appearing almost daily. Familiarity with fluorescence is a prerequisite for taking advantage of many of these developments. This review attempts to provide a framework for understanding excitation of and emission by fluorophores, the way fluorescence microscopes work, and some of the ways fluorescence can be optimized.

Line pairmath

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Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

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Linepairs per mm radiology

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by DW Holdsworth · 1990 · Cited by 75 — We have developed a low-noise digital camera based on a 512 x 96 element CCD operating in the time-delay integration mode. This camera has been combined ...

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Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases

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Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906–918 (2002).

Thanks again Rick for clarification. My previous understanding was wrong. So its as you said "numbers on the right are horizontal increments value on the left side are". So need to get horizontal increment position from left scale (lowest position of line signal profile where still can see five distinct individual peaks) and corresponding numerical value (as per right scale) is LP/MM. So right is scale is just aiding to display the left increment value and you can still gauge LP/MM just using the left scale only. When I correlate left increment position to right number only one number still remain odd is number 11. I could not match with it any left horizontal increment. Correct me if I am wrong again. How about when you are in middle or somewhere between two horizontal increment? Assuming just visual interpolation of value. Thanks.   Reply Translate  close  Dr. Uwe Zscherpel Director, BAM Berlin, Germany, Joined Jan 2010, 127 128 11:24 Dec-20-2016 https://www.ndt.net/forum/thread.php?msgID=66304#66304 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 18:07 Dec-16-2016 . Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Giloh, H. & Sedat, J.W. Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science 217, 1252–1255 (1982).

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Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, 825 N.E., 13th Street, Oklahoma City, 73104, Oklahoma, USA

Waveplates, also known as Retarders, are used to transmit light while modifying its polarization state without attenuating, deviating, or displacing the beam.

SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Panchuk-Voloshina, N. et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright photostable conjugates. J. Histochem. Cytochem. 47, 1179–1188 (1999).

Line pairphantom Radiography

Abramowitz, M., Spring, K.R., Keller, H.E. & Davidson, M.W. Basic principles of microscope objectives. Biotechniques 33, 772–781 (2002).

A quick Google search shows that some background info can be found here: https://www.nde-ed.org/EducationResources/CommunityCollege/Radiography/AdvancedTechniques/Real_Time_Radiography/ImageQualityIndicators.htm Horizontal lines on left in your photo correlate to increments of line pairs per millimeter resolution of system under test shown on the right (e.g. "I can see no better than the 5th horizontal line", so your resolution is the 5th value down on the right, or 3.5 LP/mm).   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 16:17 Dec-15-2016 https://www.ndt.net/forum/thread.php?msgID=66272#66272 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 23:08 Dec-14-2016 . Line Pair GaugeThanks Mr. Lopez for your quick response. The figure of gauge does NOT have clear instruction label which numerical scale is LP/mm and it placed on the middle so it’s created confusion and could be any of the two numerical scale. From your explanation I could understand the right numerical scale is LP/mm. Based on your explanation I could imagine if able to see 5 Individual Peaks in line profile (say at red line position) then my resolution is 3.5 lp/mm. Still not clear what is the purpose of left numerical scale and if their conversion from left to right. Most of the searched found documents lacks in clear practical instruction about its use and might assumed that they user already know how to use it.   Reply Translate  close  Rick Lopez R & D, John Deere - Tech Works Ames, USA, Joined Jul 2011, 220 17:01 Dec-15-2016 https://www.ndt.net/forum/thread.php?msgID=66274#66274 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 16:17 Dec-15-2016 . You are almost on the right track. A simple left-side to right-side horizontal line is not correct. The numbers on the right just tell the user what the horizontal increments on the left side are. For example, if your magnification system allows you to resolve as fine as the 17th increment down, then you would have 16 line-pairs per millimeter resolution.   Reply Translate  close  rhinemine Germany, Joined Dec 2016, 11 18:07 Dec-16-2016 https://www.ndt.net/forum/thread.php?msgID=66286#66286 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to Rick Lopez at 17:01 Dec-15-2016 . Thanks again Rick for clarification. My previous understanding was wrong. So its as you said "numbers on the right are horizontal increments value on the left side are". So need to get horizontal increment position from left scale (lowest position of line signal profile where still can see five distinct individual peaks) and corresponding numerical value (as per right scale) is LP/MM. So right is scale is just aiding to display the left increment value and you can still gauge LP/MM just using the left scale only. When I correlate left increment position to right number only one number still remain odd is number 11. I could not match with it any left horizontal increment. Correct me if I am wrong again. How about when you are in middle or somewhere between two horizontal increment? Assuming just visual interpolation of value. Thanks.   Reply Translate  close  Dr. Uwe Zscherpel Director, BAM Berlin, Germany, Joined Jan 2010, 127 128 11:24 Dec-20-2016 https://www.ndt.net/forum/thread.php?msgID=66304#66304 Re: Digital Radiography Line Pair Gauge Resolution Measurement In Reply to rhinemine at 18:07 Dec-16-2016 . Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Suhling, K., French, P.M. & Phillips, D. Time-resolved fluorescence microscopy. Photochem. Photobiol. Sci. 4, 13–22 (2005).

Yuste, R. & Konnerth, A., eds. Imaging in Neuroscience and Development: A Laboratory Manual. (Cold Spring Harbor Laboratory Press, New York, 2005).

Rudolf, R., Mongillo, M., Rizzuto, R. & Pozzan, T. Looking forward to seeing calcium. Nat. Rev. Mol. Cell Biol. 4, 579–586 (2003).

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Line pairphantom

Johnson, G.D. & Nogueira Araujo, G.M. A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349–350 (1981).

Hi, nicely figured out how to read the scale of the converning line pair gauge correctly! But how to measure image unsharpness or basic spatial resolution with this tpye of gauge? The only usefully parameter I found which could be meaured by this type of gauge is the pixel size of the digital image. Exactly for this measurement is was designed in the printing industry about 50 years ago! But this need a correct procedure how to convert the visible line pair resolution in LP/mm into pixel size of mm. So a pixel size of 100 micron corresponds to a maximum of 5 LP/mm. For unsharpness measurements and basic spatial resolution in digital images I recommend ASTM E 2002 or ISO 19232-5 Duplex wire IQIs and the evalustion procedures given in these standards. Best regards Uwe Z.   Reply Translate  close  SponsoredPanther 2For integrators, manufacturers, industrial labs, and NDT service providers, Panther™ 2 by Eddyfi... Technologies is the ultimate phased array ultrasonic testing (PAUT) instrument with a complete total focusing method (TFM) toolbox delivering data results faster than any other commercially available solution. With advanced inspection data results made accessible with a complete software ecosystem to suit specific needs, including an open software development kit (SDK), Panther 2 is your go-to for flexible and scalable testing requirements.➤Eddyfi TechnologiesStand12 Showcases10 Videos Product SpotlightOur pocket-sized penetrant systemOur pocket-sized penetrant system is the ideal solution when only small areas need to be inspected..., the environment does not allow for contamination or known areas need to be inspected quickly. In this case, it was a small surface on the one hand and a very sensitive environment on the other that led us to the pen system in our dialogue.➤MR Chemie GmbHStand4 Showcases4 Videos20% off for 100 daysNDT.net is celebrating reaching 100 exhibition members with a special 100-day promotion offering 20%... off on new memberships and additional ads for existing exhibition members. Don't miss this opportunity to increase your visibility in the global NDT community!➤NDT.netStand5 Showcases4 VideosMsS Guided Wave SystemMsS Guided Wave Testing Equipment and its accessoriesGuided Wave Analysis LLCStand3 Showcases9 VideosNovascan 64/128 Portable TFM and Phased Array Flaw DetectorNovascan 64/128 combines TFM, PWI (plane wave), Phased Array detection, and independent TOFD/UT de...tection (dual channels). Multiple groups of TFM and PWI (plane wave) detection can be detected simultaneously with multiple groups of PAUT, UT, and TOFD and displayed on the same screen. The instrument's unique high-end intelligent analysis technology can realize functions such as intelligent identification of defects and high-precision measurement analysis for application scenarios such as corrosion detection, weld detection, composite bonding detection, etc., greatly expanding the instrument's special application fields.➤Guangzhou Doppler Electronic Technologies INCStand40 Showcases57 Videosmore Showcases Subscribe Share... Home Exhibition Archive Forum Jobs Members Events Directory NDT A-Z Advertise Publish Privacy Policy Contact & Imprint About Follow us © NDT.net - Where expertise comes together. The Largest Open Access Portal of Nondestructive Testing (NDT)- since 1996 We use technical and analytics cookies to ensure that we will give you the best experience of our website. More Info Accept top this is debug window

Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H. & Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 12651–12656 (2002).

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