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Thorlabswaveplate

In addition to quartz, we can also supply custom zero-order mica waveplates for low power applications at visible and near-infrared wavelengths.

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Photography or image pickup with a video camera has been common in microscopy and thus a clear, sharp image over the entire field of view is increasingly required. Consequently, Plan objective lenses corrected satisfactorily for field curvature aberration are being used as the mainstream. To correct for field curvature aberration, optical design is performed so that Petzval sum becomes 0. However, this aberration correction is more difficult especially for higher-magnification objectives. (This correction is difficult to be compatible with other aberration corrections) An objective lens in which such correction is made features in general powerful concave optical components in the front-end lens group and powerful concave ones in the back-end group.

Polarization

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Achromatic Quarter-Wave plate

Objective lenses are roughly classified basically according to the intended purpose, microscopy method, magnification, and performance (aberration correction). Classification according to the concept of aberration correction among those items is a characteristic way of classification of microscope objectives.

Retardance

Below is a list of products available for purchase from CVI Laser Optics. Please contact our customer service department to place an order: ABQ-CVI Sales ABQ-CVISales@idexcorp.com

We can manufacture quartz waveplates with custom retardation for use at wavelengths from 193 to 2100 nm, and with special shapes in diameters up to 2 inches. This includes compound zero-order waveplates, multiple-order, as well as low-order waveplates for applications where a large field of view or high stability with wavelength and temperature variation is required. Depending on the laser damage threshold, environmental and cost constraints, compound waveplates may be cemented or optically contacted. Our quartz polarization rotators can also be customized for your specific wavelength, rotation angle, or size. We perform true optical measurement of retardation tolerance in-house for all of our waveplates, demonstrating our commitment to exceptional polarization control.

Circularly polarized light

An optical microscope is used with multiple objectives attached to a part called revolving nosepiece. Commonly, multiple combined objectives with a different magnification are attached to this revolving nosepiece so as to smoothly change magnification from low to high only by revolving the nosepiece. Consequently, a common combination lineup is comprised from among objectives of low magnification (5x, 10x), intermediate magnification (20x, 50x), and high magnification (100x). To obtain a high resolving power particularly at high magnification among these objectives, an immersion objective for observation with a dedicated liquid with a high refractive index such as immersion oil or water charged between the lens end and a specimen is available. Ultra low magnification (1.25x, 2.5x) and ultra high magnification (150x) objectives are also available for the special use.

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The purposes of optical microscopes are broadly classified into two; "biological-use" and "industrial-use". Using this classification method, objective lenses are classified into "biological-use" objectives and "industrial-use" objectives. A common specimen in a biological use is fixed in place on the slide glass, sealing it with the cover glass from top. Since a biological-use objective lens is used for observation through this cover glass, optical design is performed in consideration of the cover glass thickness (commonly 0.17mm). Meanwhile, in an industrial use a specimen such as a metallography specimen, semiconductor wafer, and an electronic component is usually observed with nothing covered on it. An industrial-use objective lens is optically designed so as to be optimal for observation without any cover glass between the lens end and a specimen.

Axial chromatic aberration correction is divided into three levels of achromat, semiapochromat (fluorite), and apochromat according to the degree of correction. The objective lineup is divided into the popular class to high class with a gradual difference in price. An objective lens for which axial chromatic aberration correction for two colors of C ray (red: 656,3nm) and F ray (blue: 486.1nm) has been made is known as Achromat or achromatic objective. In the case of Achromat, a ray except for the above two colors (generally violet g-ray: 435.8nm) comes into focus on a plane away from the focal plane. This g ray is called a secondary spectrum. An objective lens for which chromatic aberration up to this secondary spectrum has satisfactorily been corrected is known as Apochromat or apochromatic objective. In other words, Apochromat is an objective for which the axial chromatic aberration of three colors (C, F, and g rays) has been corrected. The following figure shows the difference in chromatic aberration correction between Achromat and Apochromat by using the wavefront aberration. This figure proves that Apochromat is corrected for chromatic aberration in wider wavelength range than Achromat is.

quarter-wave plate

A variety of microscopy methods have been developed for optical microscopes according to intended purposes. The dedicated objective lenses to each microscopy method have been developed and are classified according to such a method. For example, "reflected darkfield objective (a circular-zone light path is applied to the periphery of an inner lens)", "Differential Interference Contrast (DIC) objective (the combination of optical properties with a DIC( Nomarski）prism is optimized by reducing lens distortions)", "fluorescence objective (the transmittance in the near-ultraviolet region is improved)", "polarization objective (lens distortions are drastically reduced)", and "phase difference objective (a phase plate is built in) are available.

spatial resolution. Lines per Millimeter, or more accurately, Line Pairs per Millimeter (lp@mm) is a measure of resolution, most useful in analog imaging.

Birefringence

In the optical design of microscope objectives, commonly the larger is an N.A. and the higher is a magnification, the more difficult to correct the axial chromatic aberration of a secondary spectrum. In addition to axis chromatic aberration, various aberrations and sine condition must be sufficiently corrected and therefore the correction of the secondary spectrum is far more difficult to be implemented. As the result, a higher-magnification apochromatic objective requires more pieces of lenses for aberration correction. Some objectives consist of more than 15 pieces of lenses. To correct the secondary spectrum satisfactorily, it is effective to use "anomalous dispersion glass" with less chromatic dispersion up to the secondary spectrum for the powerful convex lens among constituting lenses. The typical material of this anomalous dispersion glass is fluorite (CaF2) and has been adopted for apochromatic objectives since a long time ago, irrespective of imperfection in workability. Recently, optical glass with a property very close to the anomalous dispersion of fluorite has been developed and is being used as the mainstream in place of fluorite.

Waveplates and polarization rotators use birefringence to transform, control, or analyze the polarization state of light. Our line of high energy quartz retarders include 90° polarization rotators, multiple-order and compound zero-order waveplates, all with highly accurate retardation. These come as standard for the most popular wavelengths from 193 – 1064 nm. Choosing the right wave retarder for a specific application can be challenging, so our technical support team is available to assist you in the decision. If your application requires more than a standard solution, we are ready to meet your needs with customized offerings.

Meanwhile, an objective lens for which the degree of chromatic aberration correction to the secondary spectrum (g ray) is set to medium between Achromat and Apochromat is known as Semiapochromat (or Flulorite).

We have extensive experience in manufacturing quartz retarders with very low transmitted wavefront error, tight parallelism and retardation control, and pristine 10 – 5 surface quality, coated with low-loss high energy antireflection coatings for maximum power handling. We can supply parts with fully custom tolerances, should they be tighter or looser, working with you to define the best set of specifications for your application. Relaxed specifications may also be appropriate for wave retarders for use at UV wavelengths or in larger sizes. Please call us to discuss your cost and performance requirements.

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** Note: The general tolerance specifications above are intended to guide determination of appropriate tolerances based both on relative cost and manufacturability. Part-specific tolerances will vary depending on size, material, shape and aspect ratio. Tighter tolerances may be possible, so please contact us to discuss your custom specifications. All specifications do not need to be from a single column, though trade-offs may be required in order to meet the desired combination of cost and performance.

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Half wave plate

An objective lens is the most important optical unit that determines the basic performance/function of an optical microscope To provide an optical performance/function optimal for various needs and applications (i.e. the most important performance/function for an optical microscope), a wide variety of objective lenses are available according to the purpose.

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