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Immersion Oil:  Clear, finely detailed images are achieved by contrasting the specimen with their medium.  Changing the refractive index of the specimens from their medium attains this contrast.  The refractive index is a measure of the relative velocity at which light passes through a material.  When light rays pass through the two materials (specimen and medium) that have different refractive indices, the rays change direction from a straight path by bending (refracting) at the boundary between the specimen and the medium.  Thus, this increases the image’s contrast between the specimen and the medium.

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The confocal microscopy facility provides researchers with the ability to visualize a variety of samples, from monolayers and small organisms, ...

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Lens Adapters. Lens mount adapters are designed to attach a lens to a camera body with non-matching mounts. Generally, a lens can be easily adapted to a ...

Angular field of view is commonly stated as the diagonal (which is the circular lens view). The angle is independent of distance (angle is the same at any ...

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Resolving power or resolution: the ability to distinguish objects that are close together.  The better the resolving power of the microscope, the closer together two objects can be and still be seen as separate.

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Parfocal: the objective lenses are mounted on the microscope so that they can be interchanged without having to appreciably vary the focus.

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One way to change the refractive index is by staining the specimen.  Another is to use immersion oil.  While we want light to refract differently between the specimen and the medium, we do not want to lose any light rays, as this would decrease the resolution of the image.  By placing immersion oil between the glass slide and the oil immersion lens (100X), the light rays at the highest magnification can be retained.  Immersion oil has the same refractive index as glass so the oil becomes part of the optics of the microscope.  Without the oil the light rays are refracted as they enter the air between the slide and the lens and the objective lens would have to be increased in diameter in order to capture them.  Using oil has the same effect as increasing the objective diameter therefore improving the resolving power of the lens.

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Total magnification: In a compound microscope the total magnification is the product of the objective and ocular lenses (see figure below).  The magnification of the ocular lenses on your scope is 10X.

Aspheric lenses are used to minimize optical aberrations within the human eye4. Parts of the eye including the tear film, cornea, and crystalline lens can ...