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The “short mount” (36.65 mm parfocal) and the “long mount” (approximately 42.5 mm parfocal) objectives were intended for microscopes of the BH era (AH, BH, CH, CK, IMT) and earlier models. For photomicrography, they should be used with the FK photo eyepieces. The eyepieces for viewing do not include an “L” in their designation, for example Bi WF10×.

The 45 mm parfocal long-barrel (LB) objectives were intended for microscopes of the BH-2 era (AH-2, BH-2, CH-2, CK2, CK40, IMT-2). For photomicrography, they should be used with the NFK photo eyepieces. The eyepieces for viewing include an “L” (indicating long barrel) in their designation, for example SWHK 10×L, WHK 10×/20 L, WHK 15×L or CWHK 10×/18L.

The specifications of the biological objectives that were designed for Olympus compound microscopes of the BH-2 era (AH-2, BH-2, CH-2, CK2, CK40, IMT-2) can be found in the Olympus LB Objective Series for Biological Use catalogue on the downloads page.

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Detailed information about the biological and metallurgical objectives that were designed for Olympus compound microscopes of the BH era (AH, BH, CH, CK, IMT) can be found in the Olympus High Quality Optics catalogue on the downloads page.

Robert T. Sutter and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

For the M, MPlan, Neo and NeoPlan 36.65 mm parfocal objectives for the BH microscope, the illuminators are part grey and part black. The BH-MA is for the brightfield M and MPlan objectives, and the BH-RLA is for the brightfield/darkfield Neo and NeoPlan objectives; the BH-RLA can be distinguished by a knob labelled “PULL BF”. The objectives are corrected for the longer tube length (210 mm) caused by the vertical illuminator. A few of these illuminators are all black, but they can be distinguished from the BH2 versions by the absence of the “∞ f=180” label.

Olympus objectiveexplained

Upright compound clinical microscopes command the second greatest market share (next to stereomicroscopes) and are sold by the thousands every year. The design of these microscopes has also received considerable attention from the manufacturers with respect to ergonomic factors. Included in the new list of features are one-handed stage and focus control, optimum eye-level positioning, low-profile stages, and rigid instrument body standards, all contributing to strain-free posture for operators while examining specimens.

The following tables are intended as a quick reference to the letters that Olympus used on its objectives in the BH and BH-2 era.

The metallurgical objectives are intended to be used with reflected light from a vertical illuminator that is fitted between the stand and the head. They can also be used with transmitted light via the substage condenser, but a vertical illuminator is still needed to provide the proper 210 mm tube length or a tube lens.

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The tutorial initializes with the automatic nosepiece feature of an ergonomically designed modern microscope (the Olympus BX45A) appearing in the window. In order to operate the tutorial, use the Swap Objectives button to toggle between the 10x and 40x objective and simulate how these components are rapidly interchanged through the use of a remote hand or foot switch.

Nikonobjective

Perhaps the most significant feature of new compound microscope designs is positioning of the stage translation and focus control knobs in an equidistant configuration from the operator (Figure 1). This allows a more relaxed posture with the hands resting comfortably on the desktop. In addition, operators are no longer required to twist their shoulders to simultaneously manipulate the stage and focus controls, considerably reducing the amount of strain associated with long-term observation. Other frequently used microscope controls, such as the field diaphragm, light intensity potentiometer, and auto-illumination photomicrography preset switch, are located in the front of the microscope base at desktop height and within easy reach of the operator (see Figure 1 for details). Push-button filter engagement levers are also positioned near the main controls to further enhance operational ease. Many microscopes are equipped with a refocusing stopper that allows quick change of specimens with an instant return to focus. In addition, coarse focus tension controls allow the operator to customize the action of z-direction stage translation to suit individual preferences. A few microscopes also offer detachable fine focusing knobs that can be fitted to either side of the instrument to suit the operator's preferences, and some knobs are coated to enhance traction and allow easy operation with only a single finger.

The ergonomic design of stress free microscopes that are easy to operate is a primary goal for all of the manufacturers. This interactive tutorial examines the automatic objective changeover design in clinical microscopes, a feature that is quickly performed with a foot or hand switch to reduce the frequency of repetitive hand motion and ease operator discomfort.

BH-RLA brightfield/darkfield vertical illuminator (for BH microscope with BH-NRE nosepiece and Neo or NeoPlan 36.65 mm metallurgical objectives)

Lowered stage assemblies, in recent instrument designs, have heights ranging from approximately five to eight inches, considerably lower than those found on previous microscopes. Many also incorporate space-saving dual pulley or pulley and guide rod systems that replace older rack and pinion mechanisms to govern stage movement. Some models feature focusing adjustments that translate the nosepiece, rather than the entire stage, to maintain a constant stage height, reducing the amount of forearm movement necessary to move the stage or change the specimen. More advanced designs eliminate protrusion of the x-directional guide from the side of the stage, thus reducing interference with focusing action. Rotational movement of modern stages often exceeds 200 degrees and some can even achieve over 250 degrees of rotation about the microscope optical axis. Any microscopist who has spent time framing images for capture on film or by digital imaging will appreciate this advanced feature, which not only saves time, but eliminates a considerable amount of frustration.

For the MA, MDPlan, MSPlan, NeoDPlan and NeoSPlan 45 mm parfocal objectives for the BH-2 microscope, there were 2 series of illuminators. The first series is all black, the BH2-MA Brightfield Vertical Illuminator for the MA, MDPlan and MSPlan objectives, and the BH2-RLA Brightfield/Darkfield Vertical Illuminator for the NeoDPlan and NeoSPlan objectives; the BH2-RLA can be distinguished by a knob labelled “PULL BF”.

New ergonomic microscope designs incorporate a two-position motorized nosepiece, which eliminates repetitive motion of rotating the nosepiece to interchange objectives. In addition, the 10x objective is often intensity equalized, so that it is not necessary for the operator to adjust the illumination intensity (lamp voltage) when changing between low and higher magnification objectives. The discomfort, and potential damage, that is caused when the eyes are exposed to sudden changes in light intensity is eliminated in this design.

Both black illuminators were replaced by the cream BH2-UMA Universal Vertical Illuminator, which can be fitted with the BH2-UBF brightfield cube and/or the BH2-UDF darkfield cube; the active cube can be selected by a sliding knob on the front. Both series of BH2 vertical illuminators include the tube lens (f = 180 mm) that is essential for these infinity-corrected objectives, and are labelled “∞ f=180”.

Objectives for the BH, BH-2 and other Olympus microscopes of this era have the series, for example “SPlan”, in front of the magnification. Objectives for the BX and other more recent Olympus microscopes have the series above the magnification, and cannot be used with older microscopes.