Fluorescencebandpassfilters

We offer a wide variety of optical filter mounts for securing and locating your optical filter.  Popular types include: Edge Grip Clamp Filter Mounts Spring Clamp Filter Holders Indexed Optical Filter Wheels Stackable Optical Filter Mounts Flip-up Optical Filter Mounts

A bandpass filter is defined by the pass band region. The two wavelengths in the bandpass where the transmittance is equal to 50% of peak transmittance are called the half-power wavelengths. The bandwidth is the difference between the half-power wavelengths which is called the full width at half maximum (FWHM). A center wavelength is defined by calculating the average of the two half-power wavelengths. The band shape is usually defined as a multiple of the BW or FWHM, and it defines the rate of attenuation with respect to wavelength change along the slopes of the bandpass.  Please see the individual product page for the transmission curve.

I suppose the best way to handle this would be to do all quantifications on the non-rotated image, and only rotate for figure presentation.

Opticalfilter

Rotations in 90 degree increments are generally safe (does not alter original data) if you have a square image. Non-square rectangles are generally safe to mirror (180 degree rotation) vertically or horizontally. Rotation in ImageJ on non-square rectangles will just result in different image dimensions.

A bandpass filter is defined by the pass band region. The two wavelengths in the bandpass where the transmittance is equal to 50% of peak transmittance are called the half-power wavelengths. The bandwidth is the difference between the half-power wavelengths which is called the full width at half maximum (FWHM). A center wavelength is defined by calculating the average of the two half-power wavelengths. The band shape is usually defined as a multiple of the BW or FWHM, and it defines the rate of attenuation with respect to wavelength change along the slopes of the bandpass.  Please see the individual product page for the transmission curve.

Opticalcomponents

Scribed and laminated soft coating construction is a commonly used construction for bandpass and edge filters. Optical filter components are coated onto separate substrate plates. These plates are then scribed to remove the film symmetrically around the required specified active film areas of the optical filters to be produced from the plate. The scribed plates are subsequently laminated together with a moisture resistant epoxy. A glass-epoxy-glass seal is formed in the scribed areas forming the best possible barrier to moisture penetration. Filters are cut through the scribed area to their finished size using diamond cutting tools. Additional moisture barriers may be applied to the cut filters if necessary to achieve extended life requirements.

Scribed and laminated soft coating construction is a commonly used construction for bandpass and edge filters. Optical filter components are coated onto separate substrate plates. These plates are then scribed to remove the film symmetrically around the required specified active film areas of the optical filters to be produced from the plate. The scribed plates are subsequently laminated together with a moisture resistant epoxy. A glass-epoxy-glass seal is formed in the scribed areas forming the best possible barrier to moisture penetration. Filters are cut through the scribed area to their finished size using diamond cutting tools. Additional moisture barriers may be applied to the cut filters if necessary to achieve extended life requirements.

I think that’s a good compromise, considering that rod-shaped cells are unlikely to line themselves up neatly! (my former lab had this issue with another tricky long sample, C. elegans ) The video example is showing the effect of successive rotations, so it is exaggerated compared to a single rotation, but it demonstrates that some information is lost with each transformation.

BandpassFilter thorlabs

Our Bandpass Filters pass a narrow spectral band of UV, visible, or NIR radiation and reject out-of-band wavelengths from X-ray to the far IR.  Applications include spectra radiometry, medical diagnostics, chemical analysis, colorimetry, astronomy, or any application where spectral isolation is required.

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What is the purpose of your image rotation? Image rotations do come at a cost (see example: https://twitter.com/haesleinhuepf/status/1088546103866388481 ).

Optical Bandpassfilter

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Our Bandpass Filters pass a narrow spectral band of UV, visible, or NIR radiation and reject out-of-band wavelengths from X-ray to the far IR.  Applications include spectra radiometry, medical diagnostics, chemical analysis, colorimetry, astronomy, or any application where spectral isolation is required.