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Type ofmicroscope

GFP stands for green fluorescent protein. GFP is a fluorescent protein that can be expressed in vivo. If GFP is exposed to light, it emits a green fluorescent signal. This property has had an enormous impact on cell biology by enabling the imaging of almost any protein, in transcription studies by working as a reporter gene, and in biochemical applications.

Can GFP antibodies detect YFP, CFP, or other derivatives?That depends on the antibody. Most GFP antibodies also recognize other derivatives, especially if there are just a few amino acid substitutions compared to GFP or EGFP. For example, the GFP-Trap recognizes AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP, S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP K206A, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP (click here for a complete list).Is GFP still fluorescent after fixation?There is no general answer to this question as it depends on the fixation method and procedure.

These objectives are designed with compensation for cover glass-thickness aberrations, making it possible to realize ideal beam spot size and quality while processing thorough a cover glass. These objective lenses can be used for laser repair processing for LCD or OLED modules.

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Whatis the purpose of the objective lens in a lightmicroscope

IR objective lens have high numerical apertures and are infinity corrected. They are used for laser machining with pulsed fundamental-harmonic YAG Lasers. Chromatic aberration is suppressed in the visible laser wavelength, achieving a high transmittance. Available with glass thickness compensation.

Endogenous proteins do not contain protein or peptide tags and therefore are sometimes difficult to detect in an assay. One solution that enables easy detection is to genetically fuse protein and peptide tags to the protein of interest. Tagged proteins can be used for purposes such as immunoprecipitation, microscopy, protein purification, Western blotting, protein arrays, etc. Proteins fused to GFP are called GFP-tagged proteins or GFP-fusion proteins. GFP-tagged proteins are often used for fluorescence microscopy, immunoprecipitation, protein purification, and Western blotting.

Left: beta-barrel structure and fluorescent core of enhanced GFP (EGFP), a GFP derivate; right: magnification of the fluorescent core, the two-ring chromophore

Labelledmicroscope

The two-ring chromophore of GFP absorbs and emits light, e.g., light photons, in the visible green spectrum.The chromophore, actually a two-ring chromophore, of GFP lies in the center of a beta-barrel structure. The two-ring chromophore is formed by oxidation and cyclization of the backbone of 3 amino acids: Threonine 65, Tyrosine 66, and Glycine 67. This process occurs during the folding of the protein and depends on different factors such as pH, temperature, and oxygen concentration.

Microscopediagram

The LRS-1064 Series of Diode-Pumped Solid-State (DPSS) Lasers are ideal for applications requiring anywhere up to 200 W of 1064 nm laser light with the ...

Diffraction-limited beams are beams with a minimum possible beam divergence for a given waist radius. That implies the maximum possible beam quality.

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Other articles where barrel distortion is discussed: aberration: …be present in a lens: barrel distortion, in which magnification decreases with distance ...

Which part of themicroscopesupports the slide that youareviewing

Whatis objective lens inmicroscope

These UV objective lens are infinity corrected and can be used for laser machining with pulsed 3rd and 4th harmonic YAG Lasers. Chromatic aberration is suppressed in both the visible and UV laser wavelength, achieving a high transmittance. Available with glass thickness compensation.

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GFP is an endogenous protein from the jellyfish Aequorea Victoria. It was isolated by Osamu Shimomura in 1962. In 1992, the sequence of GFP was cloned (Douglas Prasher) and Martin Chalfie’s lab expressed the sequence in vivo. Roger Tsien’s lab improved GFP and managed to convert it into a commonly used research tool. In 2008, The Nobel Prize in Chemistry was awarded “for the discovery and development of the green fluorescent protein, GFP.” See Roger Tsien’s Nobel Prize lecture here.

GFP is a protein and, like other proteins, it can be expressed by living organisms. Once GFP is expressed and properly folded, it shows fluorescent properties. If excited by light in the ultra-violet or blue spectrum, GFP emits green light (for more details please see sections 8 and 9). This property has had an enormous impact on cell biology: GFP and proteins fused to GFP can be detected as GFP works as a fluorescent tag. This has actually made lots of new experiments possible.4. What are GFP-tagged proteins and GFP-fusion proteins.

Many fluorescent proteins are based on the GFP sequence. These fluorescent proteins are genetically engineered and have different properties such as different excitation/emission spectra, photo or pH-stability, folding properties, half-time etc. Here is a rough overview:

Visible objective lenses are available in standard and long working distance (LWD) versions. Chromatic aberration and infinity correction is available with LWD types. The objective will allow user to focus a visible laser or perform microscopic observation of objects from a distance.

These reflective microscope objective lenses eliminate chromatic aberration over a wide bandwidth from the UV to the MWIR. They are typically used in microscope-spectrometry and failure-analysis applications in the semiconductor industry.

Chromotek offers GFP Nanobodies conjugated to beads for immunoprecipitation and unconjugated GFP Nanobodies/VHHs:GFP-Trap Agarose: anti-GFP Nanobody conjugated to agarose beadsGFP-Trap Magnetic Agarose: anti-GFP Nanobody conjugated to magnetic agarose beadsGFP-Trap Magnetic Particles M-270: anti-GFP Nanobody conjugated to magnetic particles M-270 for analysis of very large proteins/complexes.

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Apr 17, 2014 — A laser beam is not just focused light. A laser beam is coherent light. Furthermore, you cant create a laser beam by cleverly focusing ...

Nano-Trap system provides fast, reliable, and effective immunoprecipitation of fusion proteins. More than 3,000 peer-reviewed articles have already been published using ChromoTek's Nano-Traps.

Whatis the job of the objective lenses

The innovative and highly versatile lights can be customized for wavelength options. The series provides intense and highly diffused area lighting. Active area ...

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Objective lens

1. hollow and curved like the inside of a bowl noun 2. a concave surface, line, object, etc. verb transitive Word forms: conˈcaved or conˈcaving 3. to make ...

These objective lens can be used for machining with YAG lasers. A single objective can be used for all three harmonic wavelengths: 2nd HG, 3rd HG, and 4th HG. Multi-wavelength objectives are infinity corrected and have long working distances.

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May 3, 2019 — To calculate the field of view of microscope you need to know the eyepiece magnification, field number and objective lens. Once you have this ...

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Eyepiece Tube holds the eyepieces in place above the objective lens. Binocular microscope heads typically incorporate a diopter adjustment ring that allows for ...

GFP and GFP derivate EGFP have a β-barrel structure. In the center of this β-barrel, there are 3 amino acids. These acids and the cyclization and oxidation of their backbone form a two-ring chromophore.

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