The CS350B3 is an UV LED curing chamber for laboratory use and manufacture by hand. By using different UV LED lamps, CS350B3 can be used for a large.

Before diving into its function, let's first understand what a mechanical stage is. In simple terms, a mechanical stage is a platform located beneath the objective lenses of a microscope. It provides a stable and controlled surface for placing and maneuvering specimens under examination. Unlike a simple stage, which relies on manual adjustment of the slide, a mechanical stage offers precise control and movement in both the X and Y directions.

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Now, a question: What's the difference between a pinhole and the lens in a modern camera? If you want to know how a lens or a mirror makes a "real image" that can be recorded on film or a sensor, check out this older post. But basically, the lens bends light from a source so that the rays converge at a particular spot, forming a coherent image at that spot.

What does all that have to do with a pinhole camera? Instead of two LEDs, imagine you have a person standing in bright sunlight. The light hits them and reflects in all different directions. But some of it will go through the hole. Light from their head will pass through and make a spot on the bottom of the screen. Light from their feet will make a spot on the top. And so on for all the rest of their body. Put it all together and you have an upside-down image of the person.

Pinhole optics

The mechanical stage plays a vital role in the functionality of a microscope, providing precise control and movement for examining specimens with accuracy and detail. The ability to navigate across specimens in horizontal directions ensures that microscopy observations are conducted with precision. Understanding the function of the mechanical stage and its components, such as the mechanical stage knob and lower knobs, empowers users to unlock the full potential of their microscopes for scientific exploration and discovery.

Those crescents of light are essentially pinhole projections of the sun during the 2017 solar eclipse, at a moment when the sun was mostly obscured by the moon. The holes are formed by tiny gaps in the leaves of an overhead tree. Although the sun looks very small from Earth, with an angular size of about half a degree (note: never look at the sun, you fool), the images are quite large due to the distance from the "pinholes" to the ground. It's pretty cool.

Pinhole in ophthalmology

Couldn't you just use a bigger hole? Yes, but the image would be blurry. To see why, look back at the diagram with the LEDs shining through the two holes: The bigger hole made a bigger spot. If you had a human who was made of 100 colored LEDs (which would be both weird and cool) going through one big hole, there would be 100 big spots on the screen, all overlapping with one another. The tiny pinhole projects tiny spots, so we get a higher-resolution image.

People have been building image viewers with this trick for hundreds of years; it was originally known as a camera obscura, which means “dark chamber.” The idea was, you get some people in a dark room with only a tiny hole open to the outside. It seems magical, but the light from that hole would project an image of the outside world onto a wall in the room.

Pinhole lens

Of course, later on, if you didn’t want to get in a dark room, you could replace the people with chemical film, and boom: You had a pinhole camera. I’m going to go one further today and build a pinhole video camera. You can do it too! I have a video at the end of this article that will show you how, using basic materials you have at home.

Surprise! I didn't draw light rays. Instead, I made some light cones. Since the holes aren’t infinitely tiny, there are many light rays that can pass through. The light cone represents all of these rays.

Now let’s take it up a notch. What if I have two lights? Suppose I put an orange light above a green light, about 6 inches apart. What will appear on the screen? This isn't too hard to set up. I taped two LEDs to the ends of a pencil and used button cell batteries to light them up:

How to make a pinhole camera

Prisms that deviate the ray path, rotate the image, or simply displace the image from its original axis are helpful in many imaging systems. Ray deviations are ...

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Pinhole test

So, there are two big differences with an image formed from a lens. First, the hole (or aperture) can be much bigger than a pinhole. Really, you could make it as big or small as you like. This bigger size means that a lens can collect more light and form a much brighter image. You don't even need to be in a dark room to see it.

Now that we have a basic understanding of what a mechanical stage is, let's explore its function in greater detail. The primary purpose of the mechanical stage is to facilitate precise positioning and manipulation of specimens for examination. This precision is crucial for achieving accurate observations and measurements under the microscope. By allowing controlled movement in horizontal (X and Y) directions, the mechanical stage enables users to navigate across the specimen with ease, scanning different areas of interest and capturing detailed images.

Second, the lens forms an image only at a specific location. The location of the image depends on the properties of the lens (the focal length) and the distance from the object to the lens. That means your screen has to be at the location of the image in order to see anything. With the pinhole, the screen can be anywhere. If you move the screen farther from the hole, you get a bigger (and dimmer) image.

Pinhole camera model

Oh, but the big spot is on the right, just like the big hole is on the right. Why aren’t they reversed like the colors? This next diagram should make it clear. It’s a view from above showing just the green light and the green spots on the screen:

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No, the image quality isn’t great. And yes, I realize I could have just used my phone to take the video without putting it in a box. But where's the fun in that? Honestly, this is a great project for you while you’re stuck at home. In this video, I show you how to build one of your own—it only takes like five minutes—and for a bonus you get a pinhole tour of my backyard.

Admittedly, it’s a very dim image. If there’s any other light leaking into your device, you won't even be able to see the image. Why is it so dim? Because the hole is so tiny—only a little bit of light from the person makes it to the screen.

Pinhole camera Class 6

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OK, how about this: What if there’s a second, bigger hole next to the first hole—side by side. What pattern would you see on the screen with the orange and green lights? Go ahead and draw your answer somewhere, I'll wait. Meanwhile, here’s a picture of the setup:

Eyepiece Tube holds the eyepieces in place above the objective lens. Binocular microscope heads typically incorporate a diopter adjustment ring that allows for ...

Within the mechanical stage, there are specific components that contribute to its functionality, one of which is the mechanical stage knobs. These knobs, typically located on the side of the microscope stage, serve a vital role in controlling the movement of the stage. By turning one of the mechanical stage knobs, users can adjust the position of the specimen with precision on the X-axis, moving the other knob will adjust the specimen on the Y-axis. This controlled movement is essential for focusing on specific areas of the specimen and capturing detailed images at various magnifications.

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Most of the light rays go off in different directions and have their own story in some other blog post. Some of them hit the barrier and bounce off or get absorbed. But a few, at just the right angle, get through the hole to the viewing screen. These rays then reflect off the screen and give the appearance of a bright spot.

The simplest way to project an image onto a screen is with a pinhole. What the heck is a pinhole? It's a hole so tiny it has to be made with a pin. See, science isn’t that hard.

I used a piece of aluminum foil mounted to some cardboard. The foil is nice because it's easy to make holes in it, and the holes have nice clean edges. So let's see it then. Here is what I get on the screen:

Microscopes either have mechanical stage knobs located on top of the stage, or dropped down below the stage. The lower knobs below the stage operate in the same format in that one knob controls X-axis movement and the other controls Y-axis movement.

You can make a pinhole camera yourself. It's easy. Just get a box with a tiny hole such that it projects an image on the inside wall of the box. Then you need to make some type of viewing hole so you can peek inside to see the image. You have to be careful with the viewing hole, because if you let in any extra light, it will wash out the dim pinhole image.

Pinhole camera

I'm honestly surprised this picture turned out so well. I give credit to the iPhone camera's performance in low light. But why are the colors now reversed, with the green spot above the orange spot on the screen? It's easy to see with a simple light-ray diagram:

Since the orange light on top can only go through the hole one way, the only option is for its spot to be on the bottom. And vice versa for the green light. All clear now?

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But wait! There's a better way to do it. Instead of cutting a second hole, you can just put your phone inside the box, positioned so it's pointing at the screen. In fact, why stop with still images? You can use this setup to make a pinhole video camera. Here’s what the footage looks like—that’s me (upside down) waving:

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In fact, let’s go back to the simplest possible case. What happens if I have a point light source in front of a barrier with a pinhole in it. Here is a diagram: