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Fine adjustment knob: It is a tiny knob that is used for focusing on the slide accurately. This knob can make only small adjustments.

Diaphragm: It is a structure located below the stage which controls the intensity of light entering the stage. It is of two types: disc and iris.

First, the specimen must be focused under the low-resolution objective lens by coarse adjustment. Any necessary diagrams can be drawn by viewing from this resolution.

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2. My medical exams are approaching and I want to revise concepts like parts of a compound microscope better. What should I do?

Mirror: A concave mirror on one side and plain mirror, on the other hand, can be seen below the stage to focus the light on the stage for a brighter image of the specimen.

You may stick to the 80/20 rule which is also known as Pareto’s Principle. It states that 20% of your tasks/activities will bring you 80% of the results/awards. Try to find out what is more important and spend time on those. Find out these tasks and focus on them. Spend less time on tasks that may not be productive. They may entertain you now but they will not contribute as much value as other tasks would. So, manage your time as it is precious. Make sure that you are able to put yourself into action even in the most challenging and difficult times because that is how you can get closer to your dreams.

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Nose piece: The body tube has three holes that have places to fit the objective lens. This structure is called the nose piece.

The microscope must not be disturbed by tilting or shaking it while using it. This bouncing alters the light received by the lens, and the viewing of the object of focus alters.

Always use a coverslip on the slide of the specimen. This ensures other particles do not accumulate on the sample and alter the results.

Absorption and emission spectra: Ex Max: 496 nm and 564 nm,. Em Max: 612 nm. ... (using a 610/20-nm filter and the 488-nm laser). All conjugates were run at.

A compound microscope is a type of microscope that uses two sets of lenses to magnify the image under the microscope. It has an objective lens that has a resolution of 4x,10x, 40x, 100x, and an eyepiece of resolution of 10x. The usage of both lenses, compounds or magnifies the image of the object below the objective lens to achieve a resolution of 40x, 100x, 400x, 1000x.

When a person is using the coarse adjustment lens, care should be taken that the objective lens does not touch the slide of focus. Sometimes, the coarse adjustment lens, when used rapidly, may crush the specimen to be observed, and precise results cannot be obtained, and  the example and the slide also may be damaged.

© 2024 HLI Solutions, Inc. Information provided is subject to change without notice. All values are design or typical values when measured under laboratory conditions.

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The eyepiece and objective lens must be cleaned by silk cotton and transparent liquid, so that dust accumulated on the lens does not hinder the viewing of the slide or focus.

The person viewing the microscope should place the lens in a well-lit place so that an adequate amount of light strikes the concave part of the mirror under the stage.

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Eyepiece: It is the lens used on the upper part of the body tube. It usually has a resolution of 10x but can be of 5x or 15x. It is the lens through which the person views the specimens.

This principle is used to develop geometric optics, which by and large is concerned with the image-forming properties of mirrors and lenses. The basic concept ...

Body tube: It is the hollow tube at the upper arm, which has one end of the eyepiece lens, and another term has an objective lens. The body tube is altered using the adjustment knobs.

5. I am a Class 10 student, my board exams are coming soon. Can I get proper guidance and required help on the topic of parts of a compound microscope?

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The primary principle used here is the principle of compounding or magnification. The magnification factor of the eyepiece multiplied with that of the objective lens. This principle produces a magnified image.

Inclination Joint: It is the point where the pillar meets the arm. The whole microscope can tilt at this inclination joint.

Prioritizing tasks while you prepare yourself for exams is an important thing to do. You can use several techniques which will make the process easy and effective. One of the best techniques includes the ABCDE trick. This helps you a lot with prioritising tasks and is easy to do, “A" items are most important, "B" items only have minor consequences, "C" tasks have no consequences, "D" tasks can be delegated, and "E" tasks can be eliminated. Students can use this one to mark them from most important to least important and henceforth, spend more and more time on things that may have bigger impacts.

A more unobstructed view of the specimen is obtained when the coarse adjustment knob in the microscope is used. The fine adjustment knob gives an accurate picture of the sample.

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Stage: It is the rectangular structure that has a hole in the center that allows the light to pass through it. It is below the eyepiece, and most of the specimens are placed on this stage to examine.

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Objective lens: It is the lens used on the lower part of the body tube. It is attached to the nose piece. Usually, three or four nose pieces are available, which contain the objective lens of different resolutions like 10x, 40x, 100x.

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If you are using a high-resolution lens for a specimen, the fine adjustment knob should only be employed. Do not use the coarse adjustment knob as it cannot give accurate results for viewing smaller details.

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Always use a clean slide or clean the slide with proper water and wipe it so that any stains on the slide do not appear under the microscope. This can confuse the person whether the perceived object is a stain or a part of the specimen.

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If you are using an oil immersion lens, the oil should always be used for accurate results. As without the use of oil, the refractive index may be changed, which manipulates the reading of the specimen.

The person using the lens must first arrange the eyepiece. They must arrange the mirror in a manner that the maximum amount of light reaches at the end of the stage. This ensures that the specimen has adequate light for viewing.

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The resolution of the eyepiece remains constant, but the resolution of the objective lens can be changed by rotating the nose piece. Hence, the same microscope can be used to view the object of focus in different magnifications.

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The specimen must be first focused under the low-resolution lens, then later high-resolution lens must be used to target it furthermore.

Students must have thoroughly understood about what is a compound microscope, what are its parts and the working mechanism along with the precautions through the above-shared content.

If you want more resolution on the same specimen, the nose piece is turned into a higher resolution. At this point, only a fine adjustment knob should be used as a coarse adjustment knob can crush the slide.

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Clips: The stage, on the side of the specimen holder, contains clips that hold the slides on which samples will be placed.

4. I want a summary of all the important formulas that can be used while solving questions from various topics and also, diagrams from topics like parts of a compound microscope. Is it possible?

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